[RP1400] ExcelRT™ Reverse Transcription Kit II, 100 RXN
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ExcelRT™ Reverse Transcription Kit II is a complete, efficient and convenient kit to synthesize high quality first strand cDNA. This kit contains ExcelRT™ Reverse Transcriptase, which is able to synthesize the first strand cDNA at 37~50°C. The ExcelRT™ Reverse Transcriptase is a recombinant Moloney Murine Leukemia Virus (M-MLV) reverse transcriptase, which is designed to reduce RNase H activity and create better thermal stability. This kit also contains RNAok™ RNase Inhibitor, which is active against RNase A, RNase B, and RNase C. This product is supplied with optimized RT Buffer and Oligo (dT)/Random Primer Mix for highly efficient synthesis of short chain cDNA suitable for real-time PCR.
Detail
Description
ExcelRT™ Reverse Transcription Kit II is a complete, efficient and convenient kit to synthesize high quality first strand cDNA. This kit contains ExcelRT™ Reverse Transcriptase, which is able to synthesize the first strand cDNA at 37~50°C. The ExcelRT™ Reverse Transcriptase is a recombinant Moloney Murine Leukemia Virus (M-MLV) reverse transcriptase, which is designed to reduce RNase H activity and create better thermal stability. This kit also contains RNAok™ RNase Inhibitor, which is active against RNase A, RNase B, and RNase C. This product is supplied with optimized RT Buffer and Oligo (dT)/Random Primer Mix for highly efficient synthesis of short chain cDNA suitable for real-time PCR.
Features
Contains all components for reverse transcription
High yield
Thermostable, up to 50°C
Reduced RNase H ribonuclease activity
Suitable for real-time PCR
Application
Generation of first strand cDNA from total RNA or mRNA.
Suitable for generating cDNA from RNA with strong secondary structure which can be reduced at temperature up to 50°C.
Storage
-20°C for 24 months
Other Products
Congener-Independent Microcystin/Nodularins ELISA Kit (Drinking Water and Ambient Water-Compatible for use with US EPA Method 546)
Product Info
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Product Info
Format: 96-well microtiter plate (12 test strips of 8 wells)
Microcystin are a class of hepatotoxins produced by blue-green algae such as Microcystis aeruginosa. Microcystin-LR is the most common of the over 50 different congeners. Cyanobacteria can produce microcystin in large quantities during an algae bloom which then pose a major threat.
This kit can be used for Congener-Independent quantitative test of Microcystin in liquid samples such as drinking, ambient and waste water and algal cultures.
Additional Quality Control, Calibration Verification, and Spiking Solution materials can be obtained separately in optional Supplemental Pack for Method 546 (catalog # EL2024-Q).
Microcystin-LR
Informational sign postings about HABs at recreational waters: < 6 μg/L
Recreational public health advisory: 6 μg/L
Elevated recreational public health advisory (e.g. no contact): 20 μg/L
EPA 10-day drinking water health advisories:
Do Not Drink – 0.3 μg/L for bottle fed infants and preschool children, pregnant and nursing woman, elderly immunocompromised and liver conditions.
Do Not Drink – 1.6 μg/L for school-age children to adults.
Do not Use – 20 μg/L
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Format: 96-well microtiter plate (12 test strips of 8 wells)
Standards: 0 | 0.15 | 0.4 | 1 | 2 | 5 ppb
Incubation Time: 75 Minutes
Compatible for use with US EPA Method 546
Norgen’s EXTRAClean Urine Cell-Free Circulating RNA Purification Kits provide a fast, reliable, reproducible, and simple procedure for isolating circulating RNA and exosomal RNA from various urine inputs ranging from 250 μL and up to 30 mL. The purified RNA is of the highest integrity, and can be used in a number of downstream applications including real-time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extensions, and expression array assays.
EXTRAClean Cell-Free Circulating RNA Purification Mini Kit: For sample volumes ranging from 250 μL to 2 mL
EXTRAClean Cell-Free Circulating RNA Purification Midi Kit: For sample volumes ranging from 2mL to 10mL. The first column will handle the large volume input of urine that is followed by a concentration on a mini column for a final elution of 50 μL to 100 μL
EXTRAClean Cell-Free Circulating RNA Purification Maxi Kit: For sample volumes ranging from 10 mL to 30 mL. The first column will handle the large volume input of urine that is followed by a concentration on a mini column for a final elution of 50 μL to 100 μL.
All sizes, including miRNA and small RNA (< 200 nt)
Average Yields ¥
Variable depending on specimen
¥Please check page 6 of the protocol for average urine yields and common RNA quantification methods.
Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature. These kits are stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.
This kit can be used in quantitative and qualitative analysis of Pentachlorophenol residue in honey, milk, juice, and rice.
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This kit is based on ELISA technology, which is fast, easy, accurate and sensitive compared with common instrumental analysis and only needs 1.5 hours in one operation, it can considerably minimize operation error and work intensity.