RPA Isothermal Amplification Kit

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ArcticZymes RNA to DNA Ligase (ArcticZymes R2D Ligase™) is the first ligase on the market that is able to ligate DNA to 5’-phosphorylated ends of RNA in the presence of a DNA template positioning the joinable ends. With its unique substrate specificity, ArcticZymes R2D Ligase allows the development of new technologies in molecular biology research, diagnostics, and manufacturing.

Key Features:

• Novel substrate specificity
• RNA to DNA ligation
• High purity
• Detergent free
• Efficient ligation of DNA to 5’-phosphorylated ends of RNA in the presence of a DNA template positioning the ligatable ends of DNA and RNA
• ATP dependent dsDNA ligase
• Utilizes Mg²⁺ or Mn²⁺

Suggested Applications:

• RNA capturing
• In vitro transcription  
• NGS library prep
• Transcriptome amplification
• miRNA detection/analysis
• RNA splint ligation

ArcticZymes RNA to DNA Ligase (ArcticZymes R2D Ligase™) is the first ligase on the market that is able to ligate DNA to 5’-phosphorylated ends of RNA in the presence of a DNA template positioning the joinable ends. With its unique substrate specificity, ArcticZymes R2D Ligase allows the development of new technologies in molecular biology research, diagnostics, and manufacturing.

Product Description

Product Detail

Kit Storage and term of Validity

Storage term: stored at ≤-20℃,keep away from light, avoid heavy weight and repeated freezing and thawing.

Term of Validity: 14 months

Isothermal nucleic acid Principle Summary

The kit is based on room and constant temperature nucleic acid rapid amplification technology, its principle is that at room and constant temperature, the recombinase and primer form a protein/single-stranded nucleotide complex Rec/ssDNA, and invade the double-stranded DNA template with the help of auxiliary proteins and single-stranded binding protein SSB; then form a D-loop region at the invasion point and start to scan the DNA duplex, after finding the target region complementary to the primer and disintegration of the complex Rec/ssDNA, the polymerase also binds to the 3′ end of the primer to start the chain extension. The kit relies on the role of NFO enzyme and adds the designed specific molecular probes according to the template, and get the result by colloidal gold technology (sandwich method).

Technical Parameters:

Parameters	Details
Product Name	DNA Isothermal Amplification Kit NFO
Manufacturer	Amp-future
Storage Temperature	-20°C
Kit Components	Enzymes, Buffers ,Reagents
Packaging	48 Tests/box
Detection Limit	500-1000copies/µL
Shipping	ICE
Test Time	5-20mins

Isothermal nucleic acid Product Features

1/ High sensitivity and specificity, short reaction time.

2/ The reagent form is freeze-dried, stable and easy to operate.

3/ The reaction can be operated by metal bath and water bath pot without purchasing expensive PCR apparatus.

Isothermal nucleic acid Applications

Suitable for DNA isothermal rapid amplification kit(NFO type)

Primer: Require pair of nucleotide primers with the length of 25-35 bp.

DNA basic kit reaction temperature is 39 to 42℃ and time is 5-20 minutes.

Notes

1/ Please avoid nucleic acid contamination and set blank control during reaction due to the high sensitivity of the kit.

2/ Please take out the required quantity of MIRA reaction units for the experiment, and put the rest under storage conditions when performing the experiment.

Overview

• CE-IVDR marked in accordance with the European Commission Regulation (EU) No. 2017/746.
• Available for sale in Canada (Dx17200, Dx24300)
• Ideal for use in in vitro diagnostic workflows
• Extract high quality & quantity total RNA including miRNA
• No phenol step required
• Bind & elute all RNA irrespective of size or GC content, without bias
• Very sensitive & linear down to a few cells without the need for carrier RNA
• Convenient & fast spin column format
• Available in 96-Well and 96-Well (Deep Well) formats for high throughput automation
• Isolate from a wide variety of specimens
• Purified RNA is suitable for a variety of downstream applications
• Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

Norgen’s Total RNA Purification Kits Dx provide rapid methods for the isolation and purification of total RNA from tissue samples, blood, plasma, serum, saliva, bacteria, yeast, fungi and viruses for subsequent in vitro diagnostic use. Extract high quality and purity RNA with excellent RIN values and A260/A280 suitable for downstream applications including qRT-PCR, RT-PCR, microarrays, NGS and more.

Total RNA Purification Kit Dx (Spin Column)

This kit is designed to be used with any downstream application employing enzymatic amplification or other enzymatic modifications of RNA followed by signal detection or amplification. Any diagnostic results generated using the RNA isolated with Norgen’s Total RNA Purification Kit Dx in conjunction with an in vitro diagnostic assay should be interpreted with regard to other clinical or laboratory findings.

Total RNA Purification 96-Well Kits Dx (High Throughput and High Throughput Deep Well)

Thess 96-well kits provide a rapid method for the high-throughput isolation and purification of total RNA in 30 minutes using vacuum manifold, plate centrifuge, or liquid handlers with vacuum capabilities. Total RNA can be isolated from a broad range of sample sources including cultured cells, tissues, blood, serum, plasma, bacteria, yeast, fungi, and viruses.

Intended Use

To minimize irregularities in diagnostic results, suitable controls for downstream applications should be used. Norgen’s Total RNA Purification Kits Dx are intended for use by professional users such as technicians, physicians and biologists experienced and trained in molecular biological techniques. Norgen’s Total RNA Purification Kits Dx do not provide a diagnostic result. It is the sole responsibility of the user to use and validate the kit in conjunction with a downstream in vitro diagnostic assay.

NOTE: This product is not available for sale in the United States.

Details

Supporting Data

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Kit Specifications
Maximum Binding Capacity Per Well	50 μg
Maximum Loading Volume Per Well	500 μL
Size of RNA Purified	All sizes, including small RNA < 200 nt
Time to Complete 96 Purifications	30 minutes
Average Yield HeLa Cells (1 x 106 Cells) E. coli (1 x 109 cells) Brain (10 mg) Liver (10 mg) Blood (50 μL, Hamster)	15 μg 50 μg 9 μg 26 μg 1 μg
Maximum Amount of Starting Material:  Whole Blood Plasma/Serum Bacteria Yeast Fungi Plant Tissue	100 µL150 µL1 x 109 cells1 x 108 cells 40 mg 40 mg

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature.  These reagents should remain stable for at least 1 year in their unopened containers.

Component	Cat. Dx17200 (50 preps)	Cat. Dx24300 (192 preps)	Cat. Dx24350 (192 preps)	Cat. Dx24380 (576 preps)
Lysis Solution	40 mL	–	–	–
Buffer RL	–	2 x 40 mL	2 x 40 mL	350 mL
Wash Solution	22 mL	–	–	–
Wash Solution A	22 mL	2 x 38 mL	2 x 38 mL	1 x 148 mL 1 x 74 mL
Elution Solution	6 mL	–	–	–
Elution Solution A	–	2 x 20 mL	2 x 20 mL	60 mL
Mini Spin Columns	50	–	–	–
96-Well Isolation Plate	–	2	2	–
96-Well Isolation Plate (Deep Well)	–	–	–	6
Collection Tubes	50	–	–	–
96-Well Collection Plate	–	2	2	–
96-Well Collection Plate (Deep Well)	–	–	–	6
Elution Tubes (1.7 mL)	50	–	–	–
96-Well Elution Plate	–	2	2	–
96-Well Elution Plate (Deep Well)	–	–	–	6
Adhesive Tape	–	4	4	12
Product Insert	1	1	1	1