1.System optimization and adaptation:MIRA reagents have undergone a series of screenings of the entire system, types and concentrations of cofactors, and multiple optimizations of the production freeze-drying process. Based on the mastery of the components and processes, we can make adjustments according to customer needs to suit them methodology or project.
2.Diversified product forms:For example, we can provide reagents in different systems and forms (dry powder/microsphere/liquid), etc., to achieve personalized customized services according to customer needs.
3.Industrial application support: It has a 4,000-square-meter factory building to support customer projects in entrusting freeze-drying production and industrial application of the project.
Detail
Payment & Shipping Terms
Minimum Order Quantity
48T
Price
USD$3.8/T
Packaging Details
16T/Bag,48T/Box
Delivery Time
6 working days
Payment Terms
T/T, MoneyGram
Supply Ability
100000T/Month
Product Description
MIRA VS RPA,MIRA Advantages:
1.System optimization and adaptation:MIRA reagents have undergone a series of screenings of the entire system, types and concentrations of cofactors, and multiple optimizations of the production freeze-drying process. Based on the mastery of the components and processes, we can make adjustments according to customer needs to suit them methodology or project. 2.Diversified product forms:For example, we can provide reagents in different systems and forms (dry powder/microsphere/liquid), etc., to achieve personalized customized services according to customer needs. 3.Industrial application support: It has a 4,000-square-meter factory building to support customer projects in entrusting freeze-drying production and industrial application of the project.
The Ethanol (Liquid Ready) assay kit is a rapid (~5 min), method for the specific measurement and analysis of ethanol in wine, beverages, foodstuffs and other materials. Supplied as a “ready to use” liquid stable formulation that is suitable for manual auto-analyser and microplate formats and is simple, reliable and accurate.
Suitable for manual, auto-analyser and microplate formats.
Note for Content: The number of manual tests per kit can be doubled if all volumes are halved. This can be readily accommodated using the MegaQuantTM Wave Spectrophotometer (D-MQWAVE).
Biotin-PEG4-C1-alkyne is azide reactive biotinylation reagent, PEG4 arm increase aqueous solubility of the reagent. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Document
Biotin-PEG4-C1-alkyne is azide reactive biotinylation reagent, PEG4 arm increase aqueous solubility of the reagent. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
HiDi® stands for High Discrimination of mismatches at the 3’-terminus of primers in PCR. This myPOLS Biotec enzyme family is optimized for this feature and therefor, is the first choice for applications that rely on this property such as allele-specific PCR (asPCR) that is also termed allele-specific amplification (ASA).HiDi® 2x PCR Master Mix – ready to use mix simplifies your PCR setup. Only target-specific primers and template need to be added as the mix contains all components for a successful and reliable PCR. This ensures reproducible results, significantly reduces set-up times and the risk of pipetting errors.This PCR mix is also available with a full-length Taq DNA polymerase with a nuclease domain, featuring 100% compatibility with hydrolysis probes (TaqMan® probes etc.).Benchmarking with products of competitors conducted by us and others show that the HiDi® DNA polymerase family is the first choice for highly selective PCRs, such as genotyping by allele-specific PCR, HLA genotyping, analysis of single CpG methylation sites or the detection of mutations in a high background of wild-type sequences. By using HiDi® DNA polymerase, less than 10 copies of a mutation can be detected in a background of >10.000 wild-type copies straight away without any other tedious assay optimization.It has also been shown that HiDi® DNA polymerase family is highly suitable for quality control in CRISPR-Cas or TALEN-based applications.Several independently conducted studies show that HiDi® DNA polymerase is ideally suited for use in asPCR in numerous research areas ranging from mutation detection to genome editing. (read more)For research use and further manufacturing.In case you are aiming to use our RUO products as components or for your development of e.g. an IVD medical device, please contact us.
Casestudies: HiDi® DNA Polymerase: Applications from mutation detection to genome editing (read more)
Example Primer Design
Matching vs. mismatching nucleotide is placed at the 3′-end of the primer for best discrimination results.
Example Results – There´s no accounting for taste
Cilantro: some people love it in their food, some hate it. Here we are detecting a genomic SNP (rs72921001) in HeLa genomic DNA. This SNP is reported to be close to a number of genes coding for olfactory receptors. (Reference: Eriksson N. et al. (2012), “A genetic variant near olfactory receptor genes influences cilantro preference.”)
Considering, that only the C-allele specific primer is extended and yielding in a specific amplicon, we can conclude a genetic predisposition in disliking cilantro, as this SNP is significantly associated with detecting a soapy taste to cilantro.
Allele-specific PCRs were performed from 1 ng/µl of HeLa gDNA in the presence of a realtime dye, indicating the amplification of the C-allele specific primer only. The A-allele specific primer is discriminated, thus not amplified up to 50 cycles.
PCR products were subsequently analysed on a 2.5% agarose gel. Specific product is visualized by ethidium bromide staining at the amplicon length of 109 bp.
Document
HiDi® stands for High Discrimination of mismatches at the 3’-terminus of primers in PCR. This myPOLS Biotec enzyme family is optimized for this feature and therefor, is the first choice for applications that rely on this property such as allele-specific PCR (asPCR) that is also termed allele-specific amplification (ASA).
HiDi® 2x PCR Master Mix – ready to use mix simplifies your PCR setup. Only target-specific primers and template need to be added as the mix contains all components for a successful and reliable PCR. This ensures reproducible results, significantly reduces set-up times and the risk of pipetting errors.
