Introduction

This product is suitable for extracting total viral nucleic acid from cell-free/low-content cell biological samples such as body fluids, serums, plasma, soaking solutions, tissue homogenate supernatant, and culture supernatant. The Purified DNA/RNA is used for RT-PCR and PCR detection.

Details

Specifications

Principle

This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. DNA/RNA is released into the lysate. After adding magnetic particles and binding solution, DNA/RNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA/RNA was eluted by Nuclease Free Water.

Advantages

Kit Contents

Storage and Stability

This kit is shipped and stored at room temperature and is valid for 12 months.

This product is suitable for extracting total viral nucleic acid from cell-free/low-content cell biological samples such as body fluids, serums, plasma, soaking solutions, tissue homogenate supernatant, and culture supernatant. The Purified DNA/RNA is used for RT-PCR and PCR detection.

Overview

• RNA/microRNA/DNA/Proteins are preserved for more than 2 years at room temperature in Norgen’s Urine Preservative
• Compatible with most DNA, Total RNA, microRNA and protein isolation methods
• Preservative is available in a single dose liquid format (ampule)
• Preservative is also available in a dried format in tubes – Urine Collection and Preservation Tubes
• Urine Collection and Preservation Devices are available in 4 convenient sizes: 5 cc tubes, 15 cc tubes, 50 cc tubes, and 120 cc cups

Norgen’s Urine Preservative is designed for the rapid preservation of DNA, RNA, microRNA and proteins from fresh urine samples. In addition, the Urine Preservative eliminates the need to immediately process or freeze samples and allows the samples to be shipped to centralized testing facilities at ambient temperatures. The components of the Urine Preservative allow samples to be stored for over 2 years at room temperature with no detected degradation of urine DNA, RNA or proteins.

Norgen’s Urine Preservative is available in 2 convenient formats:

1.  Urine Preservative Single Dose Ampules
With this product Norgen’s Urine Preservative is supplied in a liquid format in single dose ampules. The user simply collects 5 – 50 mL of urine into a urine collection container and adds the contents of the Urine Preservative Single Dose (Cat# 18126). The urine and preservative are then mixed, and the urine nucleic acids and proteins are preserved at room temperature.

2.  Urine Collection and Preservation Tubes
Norgen’s Urine Preservative is also available in a dried format in Norgen’s Urine Collection and Preservation Tubes. The user simply collects urine into the tubes and mixes gently until the orange preservative pellet in the tube has dissolved. Norgen’s Urine Collection and Preservation Tubes are available in 3 convenient sizes:

• 5 cc tubes, 1 – 5 mL of urine
• 15 cc tubes, 5 – 15 mL of urine
• 50 cc tubes, 5 – 45 mL of urine
• 120 cc cup, 100 – 120 mL of urine

Details

Supporting Data

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Select Urine Tested with the Norgen Urine Collection and Preservation Tubes

Human
Mouse
Lynx
Wolf
Urine collected from snow

Available Sizes and Formats 

1. Urine Collection and Preservation Tubes (15 cc) – Urine inputs from 5 – 15 mL

Storage Conditions and Product Stability
All tubes should be kept tightly sealed and stored at room temperature (15 – 25°C) for up to 2 years without any reduction in performance.

Description

Specifications

Thymidylate Synthase (TS) is a crucial enzyme responsible for the synthesis of 2′-deoxythymidine-5′-monophosphate (dTMP) a precursor for thymidylate which is necessary for DNA replication and repair from 2′-deoxyuridine-5′-monophosphate (dUMP). In terms of cancer, TS is an important target for cancer treatment as the inhibition of TS and therefore nucleotide synthesis necessary for cell growth has shown to be a vital part for successful treatment against colorectal, pancreatic and breast cancers.