Saliva DNA Isolation 96-Well Kit

Overview

Sample collection is non-invasive and painless
Fast and easy high throughput processing using 96-well plates
Isolate high quality genomic DNA
Effective removal of PCR inhibitors
Compatible with preserved saliva samples collected using Norgen’s Saliva DNA Collection and Preservation Devices, as well as fresh saliva samples

This kit features a rapid, high-throughput 96-well format for isolating high yield and high quality DNA from saliva. The purified saliva DNA is compatible with any number of downstream applications including PCR, qPCR, Southern Blot analysis, sequencing, microarray analysis and more. This kit is also compatible with Norgen’s Saliva DNA Collection and Preservation Devices (Cat. RU49000). It is fully compatible to both vacuum and centrifugation protocols.

Background

Human genomic DNA found in saliva can be used in various applications in diagnostics including the detection of biomarkers to diagnose a disease, follow the disease’s progress or monitor the effects of a particular treatment. Isolation of DNA from saliva for diagnostics has become an attractive alternative to isolation from blood or tissue due to the fact that sample collection is non-invasive.

Details

Supporting Data

Figure 1 / 2

Click for expanded view

Kit Specifications
Binding Capactity Per Well	50 μg
Maximum Loading Volume Per Well	600 μL
Size of DNA Purified	All sizes
Time to Complete 96 Purifications	40 minutes

Storage Conditions and Product Stability
The kit contains ready-to-use Proteinase K which is dissolved in a specially prepared storage buffer. The Proteinase K should be stored at room temperature or 4°C. All other solutions should be kept tightly sealed and stored at room temperature (15 – 25°C). This kit is stable for 1 year after the date of shipment.

Component	Cat. RU35200 (192 preps)
Lysis Buffer F	100 mL
Proteinase K in Storage Buffer	4 mL
Binding Buffer B	40 mL
Wash Solution A	2 x 38 mL
Elution Buffer B	30 mL
96-Well Plate	2
96-Well Collection Plate	2
Adhesive Tape	4
96-Well Elution Plate	2
Product Insert	1

Features	Specifications
Main Functions	Recover DNA fragments >100bp from various pcr products, enzymatic reaction solutions, labeling reaction solutions, crude DNA, etc.
Applications	PCR, sequencing, labeling reactions, ligations and restriction digestion, etc.
Purification method	Mini spin column
Purification technology	Silica technology
Process method	Manual (centrifugation or vacuum)
Sample type	PCR products, enzymatic reaction solution
Sample amount	10-200μl
Recovery	≥85%
Elution volume	≥15μl
Time per run	≤10 minutes
Liquid carrying volume per column	800µl
Binding yield of column	35µg

Contents	D212102	D212103
Purification Times	100 Preps	250 Preps
Buffer DP	60 ml	120 ml
Buffer DW2	20 ml	50 ml
Elution Buffer	15 ml	30 ml
HiPure DNA Mini Columns II	100	250
2 ml Collection Tubes	100	250

Features	Specifications
Main Functions	Isolation circulating DNA from 0.6ml plasma,serum, body fluids
Applications	qPCR, liquid or solid chip analysis, hybridization and SNP detection, etc.
Purification method	Mini spin column
Purification technology	Silica technology
Process method	Manual (centrifugation or vacuum)
Sample type	Serum, plasma and other cell-free fluid samples
Sample amount	0.6ml
Elution volume	≥30μl
Time per run	≤40 minutes
Liquid carrying volume per column	800μl
Binding yield of column	100μg

Contents	D318002	D318003
Purification Times	50 Preps	250 Preps
Buffer ACL	40 ml	200 ml
Buffer DCW1	22 ml	110 ml
Buffer DCW2*	20 ml	2 x 50 ml
Proteinase K	34 mg	180 mg
Protease Dissolve Buffer	1.8 ml	10 ml
Carrier RNA	110 μg	310 μg
Nuclease Free Water	10 ml	30 ml
HiPure CFDNA Mini Columns	50	250
2 ml Collection Tubes	100	5 x 100

Kit Specifications
Sample Type	Fresh or frozen urine
Sample Volume Range	250 μL to 2mL
Minimum Elution Volume	50 μL
Maximum Elution Volume	100 μL
Time to Complete 10 Purifications	25–30 minutes
Size of RNA Purified	All sizes, including miRNA and small RNA (< 200 nt)
Average Yields ¥	Variable depending on specimen

Saliva DNA Isolation 96-Well Kit

Detail

Overview

Details

Supporting Data

Other Products

D2121 HiPure PCR Pure Mini Kit

Introduction

Details

Principle

D3180 HiPure Circulating Nucleic Acid Micro Kit

Introduction

Details

Principle

Advantages

EXTRAClean Urine Cell-Free Circulating RNA Purification Kits

Overview

Details

Supporting Data

Storage Conditions and Product Stability

A3P Scientific

A3P Scientific Head Office

Follow us

Component	Mini (Cat. 72900)	Midi (Cat. 72910)	Maxi (Cat. 72920)
Binding Solution K	25 mL	75 mL	1 x 75 mL 1 x 25 mL
Lysis Buffer A	30 mL	20 mL	20 mL
Wash Solution A	18 mL	18 mL	18 mL
Elution Solution A	6 mL	6 mL	6 mL
Collection Tubes	50	20	10
Elution Tubes (1.7 mL)	50	20	10
EXTRAClean Mini Spin Columns	50	20	10
EXTRAClean Midi Spin Columns	–	20	–
EXTRAClean Maxi Spin Columns	–	–	10
Product Insert	1	1	1