Overview
- Fast and easy processing using a rapid spin-column format
- Isolate high quality genomic DNA from fresh or preserved saliva samples
- Compatible with preserved saliva samples collected using Norgen’s Saliva DNA Collection and Preservation Devices (Cat. RU49000)
This kit provides a fast and simple spin column procedure for isolating genomic DNA from saliva samples collected and preserved using Norgen’s Saliva DNA Collection and Preservation Devices (Cat. RU49000), as well as fresh saliva samples.
Saliva DNA purified using Norgen’s kit is of the highest quality, and is compatible with a number of downstream research applications including PCR, Southern Blot analysis, sequencing and microarray analysis.
Background
Saliva represents an excellent non-invasive alternative to blood collection. Human genomic DNA extracted from buccal epithelial cells and white blood cells found in saliva can be used in various applications in diagnostics. Saliva DNA can be used for the detection of biomarkers to diagnose a disease, follow the diseases progress or monitor the effects of a particular treatment. Saliva DNA can also be used to diagnose particular types of infections. Isolation of DNA from saliva has become an attractive alternative to isolation from blood or tissue due to the fact that sample collection is non-invasive, the samples can be collected by individuals with little training, and no special equipment is required. Norgen’s Saliva DNA Isolation Kit provides a fast and simple procedure for isolating genomic DNA from both preserved saliva samples and fresh saliva samples.
Details
Supporting Data
Figure 1 / 3
Click for expanded view
| Kit Specifications | |
| Maximum Saliva Input | 0.5 mL preserved saliva 0.25 mL fresh saliva |
| Average Yield from 0.25 mL of Saliva* | 3 – 7 μg |
| Average Purity (OD260/280) | 1.7 – 2.1 |
| Time to Complete 10 Purifications | 30 minutes |
* Average yield will depending on the donor
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment. The kit contains a ready-to-use Proteinase K, which is dissolved in a specially prepared storage buffer. The buffered Proteinase K is stable for up to 1 year after the date of shipment when stored at room temperature.
| Component | Cat. RU45400 (50 preps) |
|---|---|
| Lysis Buffer F | 30 mL |
| Proteinase K in Storage Buffer | 1.2 mL |
| Binding Buffer B | 12 mL |
| Wash Solution A | 18 mL |
| Elution Buffer B | 15 mL |
| Spin Columns | 50 |
| Collection Tubes | 50 |
| Elution Tubes (1.7 mL) | 50 |
| Product Insert | 1 |
