The Saxitoxin (PSP) ELISA Kit is a competitive ELISA for the quantitative analysis of saxitoxin in shellfish samples. This kit can be used for rapid test of Saxition in samples such as mussels and lobster tomalley.
The Saxitoxin (PSP) ELISA Kit uses a polyclonal antibody that binds both saxitoxin and a Saxitoxin-enzyme conjugate. Saxitoxin in the sample competes with the Saxitoxin-enzyme conjugate for a limited number of antibody binding sites.
Drinking Water Thresholds:
Do not Drink – 0.2 μg/L for all
Do not Use – 3 μg/L
Other Products
Human Papillomavirus (HPV) 6/16 TaqMan PCR Detection Kits
Product Info
Document
Product Info
Overview
Detection kits for the HPV 6/16
Available in TaqMan format for analysis
More than 70 types of human papillomavirus (HPV) have been identified, and are generally classified as high-risk or low-risk depending on their relationship or lack of relationship with cancer and high-grade cervical intraepithelial neoplasia (CIN 2-3). HPV viruses are predominantly sexually transmitted and high-risk HPV types are a major risk factor for development of cervical cancer. HPV 16 has been considered as a high-risk cancer associated HPV type. The low-risk HPV type 6 has been associated with the presence of genital warts. There are many other low-risk HPV types that are not associated with genital warts or cervical cancer. Until now, HPV cannot be cultured in vitro, and immunological tests are inadequate to determine the presence of HPV cervical infection. On the other hand, biopsies can be analyzed by nucleic acid hybridization to directly detect the presence of HPV DNA.
HPV 6/16 TaqMan PCR Kit, 100 reactions
Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
Specific Primer and Probe mix for the pathogen/virus/viroid of interest
Primer and Probe mix
Positive and negative control to confirm the integrity of the kit reagents
HPV 6/16 TaqMan PCR Probe/Primer Set and Controls, 100 reactions
Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest
Nuclease-free water
Can be used together with Norgen’s PCR Master Mix (#28007) or customer supplied master mix
For research use only and NOT intended for in vitro diagnostics.
Storage Conditions and Product Stability All kit components can be stored for 2 years after the date of production without showing any reduction in performance.
All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.
Component
TaqMan Probe Cat. TM42050 (100 preps)
TaqMan Probe/Primer and Control Set Cat. TM42010 (100 preps)
Propargyl-PEG7-t-butyl ester comprises a propargyl group and a t-butyl protected carboxyl group. The propargyl group reacts with azide compounds via copper catalyzed Click Chemistry to form a stable triazole linkage. Under acidic conditions, the carboxyl group can be deprotected. The hydrophilic PEG units enhance solubility of the molecule in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Document
Propargyl-PEG7-t-butyl ester comprises a propargyl group and a t-butyl protected carboxyl group. The propargyl group reacts with azide compounds via copper catalyzed Click Chemistry to form a stable triazole linkage. Under acidic conditions, the carboxyl group can be deprotected. The hydrophilic PEG units enhance solubility of the molecule in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Short term stability: 2-8oC, Long term stability: See individual component labels
Stability:
> 2 years under recommended storage conditions
Analyte:
Digestible Starch, Resistant Starch, Total Starch
Assay Format:
Spectrophotometer
Detection Method:
Absorbance
Wavelength (nm):
510
Signal Response:
Increase
Linear Range:
4 to 100 μg of D-glucose per assay
Limit of Detection:
3.1 g/100 g
Reaction Time (min):
~ 360 min
Application examples:
Plant materials, starch samples and other materials.
The Digestible and Resistant Starch Assay Kit (K-DSTRS) for the determination of digestible, resistant and total starch in starch samples, plant and other materials.
This method is based on the research of Englyst et al. (Ref) with some modifications. Digestion is performed using saturating levels of pancreatic α-amylase (PAA) and amyloglucosidase (AMG), but in stirred containers rather than shaken tubes, to simplify sample removal.
In line with Englyst definitions:
Rapidly digestible starch (RDS) is that starch which is digested within 20 min.
Slowly digestible starch (SDS) is that starch which is digested between 20 and 120 min.
A new term, ‘Total digestible starch (TDS)’ is introduced (and measured) to cover all starch that is digested within 4 h (the average time of residence of food in the human small intestine).
Resistant starch (RS) then, is that starch which is not digested within 4 h.
The incubation conditions parallel those used in AOAC Method 2017.16, a new, rapid integrated procedure for the measurement of total dietary fiber (Megazyme method K-RINTDF). This method is physiologically based and designed to fit the definition of DF announced by Codex Alimentarius in 2009.
The Digestible and Resistant Starch Assay Kit (K-DSTRS) for the determination of digestible, resistant and total starch in starch samples, plant and other materials.
