The Saxitoxin (PSP) ELISA Kit is a competitive ELISA for the quantitative analysis of saxitoxin in shellfish samples. This kit can be used for rapid test of Saxition in samples such as mussels and lobster tomalley.
The Saxitoxin (PSP) ELISA Kit uses a polyclonal antibody that binds both saxitoxin and a Saxitoxin-enzyme conjugate. Saxitoxin in the sample competes with the Saxitoxin-enzyme conjugate for a limited number of antibody binding sites.
Drinking Water Thresholds:
Do not Drink – 0.2 μg/L for all
Do not Use – 3 μg/L
Other Products
ProbeSure™ OneStep RT-PCR Master Mix
Product Info
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Product Info
Genotype directly from RNA samples. RNA reverse transcription and cDNA PCR genotyping simultaneously in a single, one-step reaction.
About
ProbeSure OneStep RT-PCR Master Mix combines reverse transcription (RT) and PCR in a single step. This advanced master mix simplifies workflows by eliminating the need for separate reactions for reverse transcription of RNA to cDNA, and PCR amplification from the newly generated cDNA.
ProbeSure OneStep RT-PCR Master Mix is highly efficient and sensitive, enabling detection of low-abundance RNA targets, particularly useful for applications such as gene expression analysis and viral RNA detection. ProbeSure OneStep RT-PCR Master Mix demonstrates robust performance across a wide range of RNA templates and can be employed for both routine and challenging samples.
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Genotype directly from RNA samples. RNA reverse transcription and cDNA PCR genotyping simultaneously in a single, one-step reaction.
Bis-propargyl-PEG7 is a homobifunctional reagent with two propargyl groups that can be used to form triazole linkages with azide-bearing compounds or biomolecules via copper catalyzed Click Chemistry. The hydrophilic PEG units help improve the solubility of the molecule in aqueous environment. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
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Bis-propargyl-PEG7 is a homobifunctional reagent with two propargyl groups that can be used to form triazole linkages with azide-bearing compounds or biomolecules via copper catalyzed Click Chemistry. The hydrophilic PEG units help improve the solubility of the molecule in aqueous environment. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Hipure Stool RNA Kit is specially designed for stool RNA extraction. This kit is suitable for extracting high-purity microbial or host cell RNA from ≤0.1g stool samples. The kit adopts silica gel column purification technology and original solution system, which can effectively remove humic acid and other inhibitory factors in stool samples. The purified RNA can be directly used in RT-PCR, Northern hybridization and other experiments.
Details
Specifications
Features
Specifications
Main Functions
Isolation total RNA from 100-150mg stool sample
Applications
RT-PCR, Northern hybridization and other experiments
Purification method
Mini spin column
Purification technology
Silica technology
Process method
Manual (centrifugation or vacuum)
Sample type
Stool
Sample amount
100-150 mg
Elution volume
≥30μl
Time per run
≤50 minutes
Liquid carrying volume per column
100µg
Binding yield of column
800µl
Principle
The HiPure silica gel column uses a high binding ability glass fiber filter membrane as the substrate. Under the condition of high concentration of ionizing agent (such as Guanidinium chloride or guanidine isothiocyanate), the filter membrane can adsorb nucleic acid through hydrogen bonding and electrostatic and other physical factors, while protein or other impurities are not adsorbed and removed. The filter membrane that has adsorbed nucleic acids is washed to remove proteins and salts. Finally, low salt buffer solution (such as Buffer TE) or water can be used to wash out the nucleic acids adsorbed on the filter membrane. The obtained nucleic acid has high purity and can be directly used in various downstream experiments.
The stool samples are homogenized in the lysis solution, further lysed in a high-temperature water bath, and RNA is released into the lysis solution. Chloroform extraction removes genomic DNA and impurities, transfer the supernatant to an alcohol free binding solution, purify RNA through a column, and finally elute RNA with RNase Free Water. The purified RNA can be directly used for experiments such as PCR, Southern hybridization, and enzyme digestion.
Advantages
High purity – unique adsorbent for more efficient removal of inhibitors
High concentration – maximum extraction of total RNA from stool samples
Sensitive – RNA can be purified at the level of PG
Kit Contents
Contents
R418502
R418503
Purification Times
50 Preps
250 Preps
HiPure RNA Mini Columns
50
250
2ml Collection Tubes
50
250
Glass Beads (0.1~0.6mm)
30 g
150 g
Buffer SPL
30 ml
140 ml
Buffer PHC
30 ml
140 ml
Buffer GRP
60 ml
250 ml
Buffer RW1
50 ml
250 ml
Buffer RW2 *
20 ml
2 x 50 ml
RNase Free Water
15 ml
30 ml
Storage and Stability
The kit components can be stored at room temperature (15–25°C) and are stable for 18 months under these conditions. At low temperatures, Buffer SPL may form precipitates, dissolve it by 55°C water bath. After receiving the product, Buffer PHC should be stored at 2-8°C.
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Hipure Stool RNA Kit is specially designed for stool RNA extraction. This kit is suitable for extracting high-purity microbial or host cell RNA from ≤0.1g stool samples. The kit adopts silica gel column purification technology and original solution system, which can effectively remove humic acid and other inhibitory factors in stool samples. The purified RNA can be directly used in RT-PCR, Northern hybridization and other experiments.
