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ArcticZymes’ Shrimp Alkaline Phosphatase (SAP), including its recombinant version (rSAP), is the gold standard and the first heat-labile, all-purpose alkaline phosphatase on the market.
Originally isolated from Pandalus borealis (Arctic shrimp), it has been purified from a recombinant source since 2010. Unlike other alkaline phosphatases, rSAP can be fully inactivated by a short heat treatment of 15 minutes at 65°C.
This added convenience through complete heat inactivation has made SAP one of the most sold DNA modifying enzymes.
For added flexibility, especially when lyophilisation may be desired, rSAP is also available in a Glycerol FREE format. First launched in 1993, SAP’s unique features continue to make it a valuable tool in various applications.
Key Features
- Gold standard heat labile, all-purpose alkaline phosphatase.
- Completely inactivated after 5 min at 65°C or 1 min at 75°C.
- Robust: Active in most restriction enzyme buffers, no need for supplemental zinc or other additives for activity.
- Simple, hassle-free dephosphorylation of DNA, RNA, dNTPs and proteins for subsequent use in cloning or end-labelling of probes.
- No vector purification necessary.
- Easy inactivation of unincorporated dNTPs in PCR products prior to DNA sequencing or SNP analysis.
- Excellent stability at 4°C and RT.
Applications
- Dephosphorylation of vectors prior to cloning.
- Enzymatic clean-up of PCR products before sequencing.
- Ideal for MALDI-TOF, cloning, HLA typing, genotyping and sequencing.
- Preparing substrate in protein kinase studies.
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