For the detection of the SARS-CoV-2 variants with the 20I/501Y.V1, VOC-21FEB-02 and variants carrying the E484K mutation
Rapid detection of specific detection profiles
High priming efficiency
Sensitive to < 100 copies of target
Positive copy number standard curve for quantification
Accurate controls to confirm findings
96 reactions, includes master mix
Our SNPsig® kits use our own proprietary genotyping method to enable the identification of SARS-CoV-2 variants of concern. These products can be used on any real-time PCR machine using familiar protocols, whilst resulting in exceptional genotyping data.
Positive control templates for wild-type and variants are supplied in every kit to make data interpretation simple.
Our SNPsig® technology provides an alternative to sequencing as well as S gene target failure (SGTF) that enables scientists to analyse and monitor these specific genomic mutations. Our kits can provide a pivotal role in screening for SARS-CoV-2 variants for the purpose of genomic surveillance and studies.
Other Products
Human Immunodeficiency Virus (HIV) TaqMan RT-PCR Detection Kits
Product Info
Document
Product Info
Overview
Detection kits for HIV
Available in TaqMan format for analysis
Human Immunodeficiency Virus (HIV) is a retrovirus which destroys essential cells in the human immune system, such as CD4+ lymphocytes (a type of T cell), macrophages and dendritic cells. Infection with HIV therefore leads to an impaired immune system and increased susceptibility to opportunistic cancers and infections. Due to the mutating nature of HIV and damage to the T cells, the immune system is unable to fight off infections and disease. Without treatment, HIV can progress to Acquired Immunodeficiency Syndrome (AIDS). The rate of HIV progression to AIDS depends on a number of different factors including viral, host and environmental factors. However, with adherence to antiretroviral therapy (ART), many individuals with HIV will never progress to AIDS, having near normal life expectancy. Transmission of HIV is through direct contact with infected bodily fluids, either sexually; by the sharing of blood-contaminated needles or instruments; maternally through childbirth or breastfeeding; or via blood transfusion/organ transplant.
HIV TaqMan RT-PCR Kit, 100 reactions
Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
Specific Primer and Probe mix for the pathogen/virus/viroid of interest
Primer and Probe mix
Positive and negative control to confirm the integrity of the kit reagents
HIV TaqMan RT-PCR Probe/Primer Set and Controls, 100 reactions
Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest
Nuclease-free water
Can be used together with Norgen’s RT-PCR Master Mix (#28113) or customer supplied master mix
For research use only and NOT intended for in vitro diagnostics.
Storage Conditions and Product Stability All kit components can be stored for 1 year after the date of production without showing any reduction in performance.
All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.
PEG3-bis(Amino-Tri-(Propargyl-PEG8-ethoxymethyl)-methane) is large molecule with two sets of three terminal alkynes joined together by a short PEG linker. The alkynes are most frequently used in copper click chemistry with azides to form stable triazoles with the target compound. The PEG linkers as well as numerous amide bonds provide high aqueous solubility to this compound, which may alter DMPK of this compound.
Document
PEG3-bis(Amino-Tri-(Propargyl-PEG8-ethoxymethyl)-methane) is large molecule with two sets of three terminal alkynes joined together by a short PEG linker. The alkynes are most frequently used in copper click chemistry with azides to form stable triazoles with the target compound. The PEG linkers as well as numerous amide bonds provide high aqueous solubility to this compound, which may alter DMPK of this compound.
RPA/MIRA is used for DNA and RNA nucleic acid templates isothermal amplification , and can be used in the field of molecular detection of viruses, pathogenic bacteria, tissues, cells, etc.
MIRA VS PCR ,MIRA Advantages:
Low and constant temperature, low requirements on instruments
20mins
Only one pair of primers is needed
Fluorescence detection by probe method is also available
High specificity
PCR :Need to control temperature,90 minutes
LAMP:Design three pairs of primers,60minutes,Low specificity
Document
RPA/MIRA is used for DNA and RNA nucleic acid templates isothermal amplification , and can be used in the field of molecular detection of viruses, pathogenic bacteria, tissues, cells, etc.
