Rapid detection of specific detection profiles High priming efficiency Sensitive to < 100 copies of target
Positive copy number standard curve for quantification
Accurate controls to confirm findings
96 reactions
Our SNPsig® kits use our own proprietary genotyping method to enable the identification of SARS-CoV-2 variants of concern. These products can be used on any real-time PCR machine using familiar protocols, whilst resulting in exceptional genotyping data.
Positive control templates for wild-type and variants are supplied in every kit to make data interpretation simple.
SNPsig® EscapePLEX™ SARS-CoV-2 also incorporates the two gene (ORF1ab and M Gene) assay to provide a confirmatory detection of SARS-CoV-2.
DETECTION PROFILE: The E484K, K417N, K417T and P681R mutations which are found in Beta, Gamma, Delta and Delta Plus Variants of Concern
The Opentrons Flex 96-Channel Tip Rack Adapter allows the Opentrons Flex 96-channel pipette head to be compatible with Flex tips. The adapter is placed on the work table below the tip rack to ensure the rack is secured.
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The Opentrons Flex 96-Channel Tip Rack Adapter allows the Opentrons Flex 96-channel pipette head to be compatible with Flex tips. The adapter is placed on the work table below the tip rack to ensure the rack is secured.
Storage term: stored at ≤-20℃,keep away from light, avoid heavy weight and repeated freezing and thawing.
Term of Validity: 14 months
Isothermal nucleic acid Principle Summary
The kit is based on room and constant temperature nucleic acid rapid amplification technology, its principle is that at room and constant temperature, the recombinase and primer form a protein/single-stranded nucleotide complex Rec/ssDNA, and invade the double-stranded DNA template with the help of auxiliary proteins and single-stranded binding protein SSB; then form a D-loop region at the invasion point and start to scan the DNA duplex, after finding the target region complementary to the primer and disintegration of the complex Rec/ssDNA, the polymerase also binds to the 3′ end of the primer to start the chain extension. The kit relies on the role of NFO enzyme and adds the designed specific molecular probes according to the template, and get the result by colloidal gold technology (sandwich method).
Technical Parameters:
Parameters
Details
Product Name
DNA Isothermal Amplification Kit NFO
Manufacturer
Amp-future
Storage Temperature
-20°C
Kit Components
Enzymes, Buffers ,Reagents
Packaging
48 Tests/box
Detection Limit
500-1000copies/µL
Shipping
ICE
Test Time
5-20mins
Isothermal nucleic acid Product Features
1/ High sensitivity and specificity, short reaction time.
2/ The reagent form is freeze-dried, stable and easy to operate.
3/ The reaction can be operated by metal bath and water bath pot without purchasing expensive PCR apparatus.
Isothermal nucleic acid Applications
Suitable for DNA isothermal rapid amplification kit(NFO type)
Primer: Require pair of nucleotide primers with the length of 25-35 bp.
DNA basic kit reaction temperature is 39 to 42℃ and time is 5-20 minutes.
Notes
1/ Please avoid nucleic acid contamination and set blank control during reaction due to the high sensitivity of the kit.
2/ Please take out the required quantity of MIRA reaction units for the experiment, and put the rest under storage conditions when performing the experiment.
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Storage term: stored at ≤-20℃,keep away from light, avoid heavy weight and repeated freezing and thawing.
