Exceptional value for money
Rapid detection of all clinically relevant subtypes
Positive copy number standard curve for quantification
Highly specific detection profile
High priming efficiency
Broad dynamic detection range (>6 logs)
Sensitive to < 100 copies of target
Accurate controls to confirm findings
Spring Viremia of Carp is caused by Rhabdovirus carpio, a bullet-shaped RNA virus. The disease has been reported in common carp (or koi) (Cyprinus carpio), grass carp (Ctenopharyngodon idella), bighead carp (Aristichthys nobilis), silver carp (Hypophthalmichthys molitrix), and Crucian carp (Carassius carassius), a close relative of the goldfish. Recent evidence suggests that common goldfish (C. auratus) are also susceptible. The disease was initially diagnosed in Yugoslavia (Fijan et al. 1971). Since then, it has been identified in other European countries, Russia, and the Middle East. Mortality has reached 70% in yearling carp from European populations. Adult fish can also be affected but to a lesser degree.
Other Products
D3115 HiPure Tissue&Blood DNA Maxi Kit
Product Info
Document
Product Info
Introduction
This product provides fast and easy methods for purification of total DNA for reliable PCR and Southern blotting. Total DNA (e.g., genomic, viral, mitochondrial) can be purified from whole blood, tissue and culture cells.
Details
Specifications
Features
Specifications
Main Functions
Isolation total DNA from 10ml blood and 1g tissue using Maxi column
Applications
PCR, southern bolt and virus detection, etc
Purification method
Maxi spin column
Purification technology
Silica technology
Process method
Manual (centrifugation or vacuum)
Sample type
Tissue, cell, blood, saliva, swab, blood spot, semen and other clinical samples
Sample amount
3-10ml
Elution volume
≥700μl
Time per run
≤90 minutes
Liquid carrying volume per column
4ml
Binding yield of column
5mg
Principle
This product is based on silica column purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption column. Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, Nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).
Advantages
High quality DNA – meet a variety of downstream applications, including PCR, qPCR, enzyme digestion, hybridization, etc.
Fast – without separation of leukocytes, organic extraction or ethanol precipitation
Simple – all nucleic acids can be obtained by direct digestion
Pertinence – specially designed for isolating DNA from 3-10ml blood and related body fluids
Wide applicability – handle a variety of liquid samples
Proteinase K, RNase A should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
*Note：Leukocyte protocol can be used when large volume whole blood samples need to be processed. Whole blood was treated with red blood cell lysate, and white blood cells were obtained by centrifugation before extraction
Select the right purification kit to get impactful results：
This product provides fast and easy methods for purification of total DNA for reliable PCR and Southern blotting. Total DNA (e.g., genomic, viral, mitochondrial) can be purified from whole blood, tissue and culture cells.
Rapid isolation of both small and large species of DNA from urine
Convenient spin column format
Effective removal of PCR inhibitors
Purified DNA is highly suited to sensitive downstream applications
Allows for the purification of viral DNA from urine
Both high molecular weight DNA (greater than 1 kb in size; mostly cell associated) and the smaller DNA (150 – 250 bp; derived from the circulation) is effectively isolated and purified using a rapid and convenient spin column protocol. This kit can be used to isolate DNA from a broad range of viruses in urine as well. Salts, metabolic wastes, proteins and other contaminants are removed to yield inhibitor-free DNA for use in sensitive applications. The DNA is of excellent quality for various downstream applications such as PCR, qPCR and DNA fingerprinting, methylation studies and more.
This kit provides a fast, reliable and simple procedure for isolating DNA from urine volumes ranging from 50 μL to 1.75 mL of urine. Multiple samples can be processed in 30 minutes.
Urine DNA Isolation Kit (Slurry Format)
This kit provides a fast, reliable and simple procedure for isolating DNA from urine volumes ranging from 3 mL to 25 mL. Multiple samples can be processed in 30 minutes. Multiple samples can be processed in 45 minutes.
Urine DNA Isolation Maxi Kit (Slurry Format)
This kit provides a fast, reliable and simple procedure for isolating DNA from urine volumes ranging from 25 mL of urine up to 80 mL. Multiple samples can be processed in 45 minutes.
Background
DNA found in urine can be divided into 2 basic categories. The larger species, genomic-DNA (gDNA), is generally greater than 1 kb in size, and appears to be derived mainly from exfoliated cells. The second species is smaller, generally between 150 and 250 bp (apoptotic-DNA), and derives, at least in part, from the circulation. The second species is also considered as an RNA/DNA hybrid as reported by Halicka et al. (2000). Both types of DNA can be isolated reliably using this kit.
30 minutes hands-on time (plus a 1 hour incubation)
* Yield will vary depending on the type of sample processed
Storage Conditions and Product Stability All buffers should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. The kit contains a ready-to-use Proteinase K, which is dissolved in a specially prepared storage buffer. The buffered Proteinase K is stable for up to 2 years after the date of shipment when stored at room temperature.
Convenient – With the ready-to-use Master Mix, the user needs only to add template to the master mix and enzyme in order to set up the reverse transcriptase reaction
Time Savings – Set up RT reactions in a shorter time since less pipetting steps are required
Cost Efficient – No need to buy separate enzymes, dNTPs and buffers. All are included with the ready-to-use Master Mix kits
High Sensitivity and Yield – the optimized Master Mix allows for highly sensitive amplifications with high yields of PCR products
Robust Enzyme – broad range of working temperatures from 37°C to 60°C. Capable of amplifying difficult templates with a high degree of reproducibility
TruScript Reverse Transcriptase is Available in Three Convenient Formats:
This kit contains 5X RT Buffer and a vial of TruScript Enzyme Mix (200 units/µL). This enzyme can be used for reverse-transcription reactions with any user-supplied primers.
2. TruScript First Strand cDNA Synthesis Kit (Cat.#54420)
This is an all-in-one, ready-to-use product for simple set-up of reverse transcription of total RNA (both poly A- or non-poly A-containing transcripts).
The kit contains the 2x Reaction Mix and the TruScript Enzyme Mix. The 2x Reaction Mix contains a blend of buffer, nucleotides and primers (oligo dT and random hexamers) for effective cDNA synthesis from total RNA transcripts.
3. TruScript First Strand cDNA Synthesis Kit for mRNA (Cat.#54400)
This is an all-in-one, ready-to-use product for simple set-up of reverse transcription of messenger RNA (poly A-containing transcripts).
The kit contains the 2x Reaction Mix and the TruScript Enzyme Mix. The 2x Reaction Mix contains a blend of buffer, nucleotides and oligo dT primer for the effective cDNA synthesis from total RNA transcripts or enriched mRNA sample.
Which TruScript Kit is Best for Your Needs?
TruScript Reverse Transcriptase
TruScript First Strand cDNA Synthesis Kit for mRNA
TruScript First Strand cDNA Synthesis Kit
Kit contains only reverse transcriptase enzyme and buffer (no primers or other buffer components)
Your template is enriched mRNA
Your template is total RNA (poly A OR non-poly A-containing transcripts)
Kit contains oligo dT
Kit contains both oligo-dT primer and Random Hexamers
Kit contains reaction buffer with nucleotides and primer
with oligo-dT
with oligo-dT and random hexamers
You have your own first strand synthesis primer
Your template is microRNA (using your own primers)
Norgen’s TruScript Reverse Transcriptase and the 5x RT Buffer should be stored at -20°C. These reagents should remain stable for at least 1 year in their unopened containers.
