Overview
- DNA can be isolated and detected from as little as 100 µL of sputum
- Effective removal of PCR inhibitors
- Compatible with Norgen’s Saliva Collection and Preservation Device (Cat. RU49000)
- Sputum Liquification Buffer (Cat. 28289) can be purchased separately
This kit constitutes an all-in-one system for the rapid isolation of DNA from sputum samples. It allows for the isolation of bacterial or eukaryotic DNA from the sputum samples using spin-column chromatography based on Norgen’s proprietary resin. The kit includes all the reagents needed to process sputum DNA. The protocol can be completed in 30 minutes. Purified DNA is of the highest quality and free from inhibitors, and can be used in sensitive downstream applications including PCR and qPCR.
Background
A sputum specimen is the name given to the mucus which is expectorated from the lower airways. High quality sputum samples should contain very little saliva, as this may contaminate the sputum sample with oral bacteria. Sputum samples are typically evaluated to look for infections such as Moraxella catarrhalis, Mycobacterium tuberculosis, Streptococcus pneumoniae ;and Haemophilus influenza. Other pathogens can be detected in sputum as well, including HIV. In addition, sputum samples are useful in the detection of lung cancer or to evaluate chronic inflammation.
Details
Supporting Data
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| Kit Specifications | |
| Time to Complete DNA Isolation | < 1 hour |
| Average Yield of DNA from 1.0 mL* | 75 μg |
| Average Purity (OD260/280) | 1.8 – 2.1 |
* Average DNA yield will vary depending on the health status of the donor
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
Norgen’s Sputum DNA Isolation Kit contains ready-to-use Proteinase K, which is dissolved in a specially prepared storage buffer. The buffered Proteinase K is stable for up to 2 years after the date of shipment when stored at room temperature.
| Component | Cat. 46200 (25 preps) |
|---|---|
| Slurry D | 55 mL |
| Proteinase K in Storage Buffer | 0.6 mL |
| Solution WN | 4 mL |
| Wash Solution BE | 9 mL |
| Elution Buffer B | 8 mL |
| Mini Filter Spin Columns | 25 |
| Collection Tubes | 25 |
| Elution Tubes (1.7 mL) | 25 |
| Product Insert | 1 |
