Staphylococcus Enterotoxin B (SEB) Lateral Flow Detection Kit
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Rapid dipstick test for detecting Staphylococcus Enterotoxin B (SEB) in liquid samples
Detects 10 ppb and greater Staphylococcus Enterotoxin B (SEB) in the sample
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Rapid dipstick test for detecting Staphylococcus Enterotoxin B (SEB) in liquid samples
Detects 10 ppb and greater Staphylococcus Enterotoxin B (SEB) in the sample
The contamination of pathogenic microorganisms and their toxins in food and water is a serious issue for human health and safety. For instance, enterotoxins (SEs) produced by Staphylococcus aureus (S. aureus) are heat-stable, meaning pathological activity remains even after exposure to sterilization techniques and digestive proteases. Among the SEs, staphylococcal enterotoxin A (SEA) and B (SEB) are confirmed toxins which cause enteritis and food poisoning. Symptoms include nausea, vomiting, diarrhea, which in severe cases, may lead to fatalities in children and the elderly. These SEs, known as superantigens, non-specifically activate Tcells, leading to proliferation which ultimately results in T-cell elimination. This activation directly and indirectly induces a massive release of inflammatory cytokines.
In addition to acute poisoning, researchers reported that these toxins may play roles in the pathogenesis of autoimmune diseases. More specifically, intestinal dysbiosis (enteromicrobial imbalance) was found in patients with rheumatoid arthritis (RA) and which may overwhelm the host immune defense functions by chronic exposure to excess amounts of these pathogens (7, 8). In animal models, SEs synergistically play a role in the pathogenesis of autoimmune-related diseases, such as atopic dermatitis, food allergies, colitis, arthritis, and systemic lupus erythematosus.
Attogene is offering this highly robust test to detect SEB by lateral flow in diverse liquid sample types.
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Purification and enrichment of intact exosomes from plasma, serum, urine, cell culture media and saliva in less than 30 minutes.
Versatile sample input ranging from 250 µL to 30 mL
Urine Exosome Purification Mini Kit (250 µL – 1 mL urine)
Urine Exosome Purification Midi Kit (2 mL – 10 mL urine)
Urine Exosome Purification Maxi Kit (11 mL – 30 mL urine)
Exosome purification is based on Norgen’s proprietary resin separating matrix through exosomes’ surface proteins.
No precipitation reagents, overnight incubation, protease or coagulant treatments required
No time-consuming ultracentrifugation, filtration or special syringes required
Purify intact exosomes with a size ranging from 40-200 nm depending on sample input type
Purified exosomes are compatible with functional studies.
Purified exosomes are free from any protein-bound cell-free circulating RNA
Purified exosomes are compatible with NanoSight® or Electron Microscopy for assessing the approximate exosome size range and concentration.
Exosomal RNA can be extracted from the purified exosomes using Norgen’s Exosomal RNA Purification technology or any other RNA extraction method.
The Urine Exosome Purification Kits provide a fast, reliable and convenient method to purify and enrich for pure intact exosomes from different urine sample volumes ranging from 250 µL to 30 mL. These kits also allow for the purification of intact extracellular vesicles (EVs) from different urine sample volumes, and these EVs are ready for any downstream application. The purification is based on Norgen’s proprietary resin.
These kits provide a clear advantage over other available methods since they do not require any special instrumentation, ultracentrifugation, precipitation reagents or any protease treatments. More importantly, the purified exosomes will not be contaminated with any other RNA-binding proteins that may contaminate your exosomal RNA, which is essential if studying exosomal RNA gene expression.
NanoSight® Analysis
Exosomes enriched with Norgen’s Urine Exosome Purification Kits can be analyzed using NanoSight® for assessing the approximate exosome size range and concentration.
Exosomal RNA Analysis
Exosomal RNA can be isolated using Norgen’s Exosomal RNA Isolation Kit from exosomes enriched using Norgen’s Urine Exosome Purification Kits for gene expression analysis using RT-qPCR, microarray or NGS and for Biomarker discovery.
Storage Conditions and Product Stability All buffers should be kept tightly sealed and stored at room temperature. These kits are stable for 2 years after the date of shipment.
Important Note Urine samples stored at -70°C, -20°C or at 4°C will develop some precipitation due to the aggregation of some of the highly abundant proteins in urine. Eliminating these precipitates using centrifugation or filtration may cause the loss of exosomes. Furthermore, these precipitates may affect the quality of the purified nucleic acid. We recommend the use of Norgen’s Urine Preservative when collecting urine samples, which is designed for the preservation of nucleic acids and proteins in fresh urine samples at ambient temperatures. The components of the Urine Preservative allow samples to be stored for over 2 years at room temperature with no detected degradation of urine DNA, RNA or proteins. Norgen’s Urine Preservative is available as a liquid format in Norgen’s Urine Preservative Single Dose Ampules, as well as in a dried format in Norgen’s Urine Collection and Preservation Tubes.
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