The Starch Damage Test Kit is suitable for the determination of starch damage in wheat flour / cereal flours.
Detail
K-SDAM
SKU: 700004338
200 assays per kit
Content:
200 assays per kit
Shipping Temperature:
Ambient
Storage Temperature:
Short term stability: 2-8oC, Long term stability: See individual component labels
Stability:
> 1 year under recommended storage conditions
Analyte:
Starch Damage
Assay Format:
Spectrophotometer
Detection Method:
Absorbance
Wavelength (nm):
510
Signal Response:
Increase
Limit of Detection:
0.5 g/100 g
Total Assay Time:
~ 40 min
Application examples:
Cereal flours and other materials.
Method recognition:
AACC Method 76-31.01, ICC Standard No. 164 and RACI Standard Method
The Starch Damage Test Kit is suitable for the determination of starch damage in wheat flour / cereal flours.
The milling of wheat causes physical damage to a proportion of the starch granules of the flour. The level of starch damage directly affects water absorption and dough mixing properties of the flour and is thus of technological significance.
All reagents stable for > 2 years after preparation
Only enzymatic kit available
Very specific
Simple format
Mega-Calc™ software tool is available from our website for hassle-free raw data processing
Standard included
Other Products
H5N1 TaqMan RT-PCR Detection Kits
Product Info
Document
Product Info
Overview
Detection kits for H5N1
Available in TaqMan format for analysis
Influenza virus infection of birds, humans and other animals is a major public health problem worldwide. Influenza viruses are classified as either type A, B or C based on differences in their nucleoproteins and matrix proteins. The type A viruses are the most virulent human pathogens among the three influenza types and cause the most severe disease and epidemics. The different types can be further classified into subtypes based on antigenic differences in two surface glycoproteins; hemagglutinin and neuroamidase. All known subtypes of influenza A can be found in birds (H1-H16, N1-N9), while a limited number of the subtypes have been found in humans (H1-H3, N1 and N2). However, over the past few years, various subtypes of Influenza A viruses, including H5N1, have been reported to infect humans (WHO, 2006). In addition, the coexistence of human influenza viruses and avian influenza viruses may provide an opportunity for genetic material to be exchanged between these viruses. This could potentially create a new virulent influenza strain that is easily transmissible and lethal to humans (Food Safety Research Information Office, 2006). Thus, there is the need for sensitive diagnostic tests to allow for the rapid and early detection of these H5 influenza virus infections, to help reduce the risk of epidemics or pandemics in both animals and humans.
H5N1 TaqMan RT-PCR Kit, 100 reactions
Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
Specific Primer and Probe mix for the pathogen/virus/viroid of interest
Primer and Probe mix
Positive and negative control to confirm the integrity of the kit reagents
H5N1 TaqMan RT-PCR Probe/Primer Set and Controls, 100 reactions
Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest
Nuclease-free water
Can be used together with Norgen’s RT-PCR Master Mix (#28113) or customer supplied master mix
For research use only and NOT intended for in vitro diagnostics.
Storage Conditions and Product Stability All kit components can be stored for 1 year after the date of production without showing any reduction in performance.
All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.
Short term stability: 2-8oC, Long term stability: See individual component labels
Stability:
> 2 years under recommended storage conditions
Analyte:
Pullulanase/Limit-Dextrinase
Assay Format:
Spectrophotometer
Detection Method:
Absorbance
Wavelength (nm):
400
Signal Response:
Increase
Limit of Detection:
0.18 U/mL for pullulanase preparations (50-fold dilution) 0.01 U/g for limit dextrinase in milled malt
Reproducibility (%):
~ 3%
Total Assay Time:
~ 10 min (Pullanase), ~ 30 min (Limit-Dextrinase)
Application examples:
Assay of microbial pullulanase preparations. Measurement of limit-dextrinase in malt extracts.
Method recognition:
Novel method
PullG6 assay for the measurement of pullulanase employs a water soluble defined substrate, namely 4,6-O-benzylidene-4-nitrophenyl-63-α-D-maltotriosyl-maltotriose (BPNPG3G3), coupled with the ancillary enzymes α-glucosidase and β-glucosidase. Upon hydrolysis of the substrate at the 1,6-α-linkage by pullulanase or limit-dextrinase, the released 4-nitrophenyl-β-maltotrioside is immediately hydrolysed to glucose and 4-nitrophenol by the concerted action of the α-glucosidase and β-glucosidase enzymes in the reagent mixture. The reaction is terminated and phenolate ions are developed by addition of dilute alkali. The absorbance is read at 400 nm and the value obtained correlates directly with pullulanase activity.
Permagen’s 15 mL / 50 mL Adapter Plate was designed with automation in mind. The SBS/ SLAS footprint (127.75mm x 85.50mm) will act as an insert on your automated liquid handler allowing any of our Centrifuge Tube racks to essentially become ready for automation
Allows the transfer from large volumes into microplates etc. where magnetic separation cannot be achieved
Features include solid aluminum alloy design with hard coat finish for years of trouble-free use, and simple integration
Works with any of our six position Centrifuge Tube Racks
Document
Permagen’s 15 mL / 50 mL Adapter Plate was designed with automation in mind. The SBS/ SLAS footprint (127.75mm x 85.50mm) will act as an insert on your automated liquid handler allowing any of our Centrifuge Tube racks to essentially become ready for automation
