Mastitis is the single most costly disease of dairy cattle resulting in the reduction of milk yield and quality. The inflammation of the utter is mainly caused by infection of various bacteria. Streptococcus uberis is a gram-positive bacterium that is known worldwide as an environmental pathogen responsible for a high proportion of cases of mastitis in lactating cows and is also the predominant organism isolated from mammary glands during the non-lactating period. Often it is resistant to treatment and causes persistent high somatic cell counts without clinical mastitis.
Storage Conditions and Product Stability All kit components can be stored for 2 years after the date of production without showing any reduction in performance.
All kit components should be stored at -20°C upon arrival.
Component
Cat. TM30850 (100 preps)
Cat. TM30810 (100 preps)
MDx TaqMan 2X PCR Master Mix
2 x 700 μL
–
S. uberis Primer & Probe Mix
280 μL
280 μL
S. uberis Positive Control
150 μL
150 μL
Nuclease-Free Water (Negative Control)
1.25 mL
1.25 mL
Product Insert
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Other Products
NGS DNA Fragmentation & Library Prep Kit (illumina and MGI Platforms)
Product Info
Document
Product Info
The NGS DNA Fragmentation & Library Prep Kit (illumina and MGI Platforms) was developed for construction of high quality libraries for next generation sequencing. The kit uses intact genomic DNA (EDTA-free DNA or DNA resuspended in TE buffer) as input DNA without an additional DNA fragmentation step. Our technology provides a fast and simple workflow. DNA libraries can be generated around 2 hours with only 10 min hands-on time. Library multiplexing is possible.
NGS DNA Fragmentation & Library Prep Kit Workflow
The incorporation of DNA fragmentation in the kit makes it possible to directly use intact genomic DNA as input DNA without the need of mechanical DNA shearing or enzymatic DNA fragmentation. The NGS DNA Fragmentation & Library Prep Kit does not generate sequencing bias as compared to library using mechanical sheared DNA as input. Sequence coverage is also consistent between enzymatic shearing and mechanical shearing. The library size is inversely correlated with the incubation time of step 1 at 20°C.
Three index types are available for the kit of the illumina platform:
Non-index (Cat.# 30026): Libraries do not have index.
Index (Cat.# 30028): Each of the index primers contains a unique index sequence of 6 bases. Library multiplexing for 48 samples is possible. Index information can be downloaded here.
Unique dual index (Cat.# 30030): Library multiplexing for 96 samples is possible. With the unique features of our 4-Base Difference Index System, the index sequence is 8-base long and each index has 4 bases different from others. Our unique dual index primers effectively identify sequencing errors such as index hopping, mis-assignment of reads, and de-multiplexing errors etc. The unique dual index primers set consists of 96 pre-mixed unique pairs of index primers. Index information can be downloaded here.
Indexes are available for the MGI platform kits (Cat.# 34028).
Kit features:
1.5-hour protocol from intact genomic DNA to NGS library
Intact genomic DNA as input, DNA fragmentation is not needed.
Works with both EDTA-free DNA and DNA resuspended in TE buffer
Library conversion efficiency: 100 ng, 300 ng and 500 ng of intact genomic DNA were used as input.
The library size is inversely correlated with the incubation time of step 1 at 20°C.
NGS data comparison: enzymatic shearing versus mechanical shearing
Enzymatic shearing • DNA shearing and library prep: BioDynami NGS DNA Fragmentation & Library Prep Kit Mechanical shearing • DNA shearing: Covaris sonication • Library prep: BioDynami NGS DNA Library Prep Kit.
Document
The NGS DNA Fragmentation & Library Prep Kit (illumina and MGI Platforms) was developed for construction of high quality libraries for next generation sequencing. The kit uses intact genomic DNA (EDTA-free DNA or DNA resuspended in TE buffer) as input DNA without an additional DNA fragmentation step. Our technology provides a fast and simple workflow. DNA libraries can be generated around 2 hours with only 10 min hands-on time. Library multiplexing is possible.
Silicone oil thermal conductive shelf, high-precision processing method of upper and lower clamping, shelf temperature uniformity can reach ±0.5°c
Integrated cavity:
Integrated chamber, the shelf and cold trap are located in the same chamber, the steam conduction is faster and the freeze-drying effect is better
Refrigerating and heating control system:
Using thermally conductive silicone oil with a high temperature range and precise PID control algorithm, the control accuracy can reach ±0.5°C. Sample shelf temperature range: -70°C~ +80°C
Freeze-drying endpoint testing system:
The pressure comparison method is used to determine the freeze-drying end point. The freeze-drying end point test can be automatically performed after the analytical drying stage to ensure that the moisture content of the material reaches the standard requirements.
Vacuum precise control and adjustment system:
The sublimation and analytical drying processes are controlled and adjusted with high precision to make the freeze-drying process warmer and the sample sublimation more reliable.
Pulse backfill system:
Three backfilling modes can be selected: slow, medium and fast, which avoids the problem of effective substances being blown away and lost due to the aeration of granular and flocculent materials after freeze-drying.
Manual automatic mode selection:
Manual mode is used to explore process parameters (strong human intervention), automatic mode is used in the mature stage of the process, one-click operation, simple and convenient
Data recording and analysis system:
Through the PC host computer, all operating parameters and background parameters of the equipment can be recorded in real time, and all parameter action changes and action changes of operating components can be recorded.
Freeze-drying process recipe storage function:
The host can store 60 sets of fixed or user-defined freeze-drying process recipes, and the PC can store >10,000 programs (depending on the computer hard drive)
Eutectic point test function:
The eutectic point temperature of the product can be tested offline and online, and the spectrum can be analyzed manually or automatically (optional)
Remote control system:
The operating status of the device can be monitored remotely, and the device can be monitored in real time via WiFi or the cloud.
Mobile phone monitoring system:
You can monitor the operating status of the equipment with your mobile phone, which is simple and convenient (optional)
Cold trap defrost system:
The hot air defrosting method has high safety performance; the defrosting speed is fast, which solves the inconvenience caused by traditional immersion defrosting.
Ractopamine ELISA Test Kit is a competitive enzyme immunoassay for detection in meat, feed, liver, kidney, milk, urine, serum and plasma. Ractopamine has been banned for use as a livestock growth promoter in many countries around the world. To prevent ractopamine residues from entering the food chain, both producers and government surveillance agencies need technologies and methods that can provide rapid, accurate and reliable detection at specific sensitivities. This kit enables government agencies, food manufacturers, as well as quality assurance organizations, to detect ractopamine as low as 0.1 ng/g or ppb level in a variety of sample types.
