Sulfo DBCO-amine

Introduction

Intended Use

For the isolation and differentiation of sorbitol-negative Escherichia coli serotype O157 from food and animal feed and other materials

Principle and Interpretation

Enzymatic digest of casein and enzymatic digest of animal tissue provides nitrogen source, vitamins and growth factors; sodium chloride maintains balanced osmoticpressure; sorbitol is a fermentable sugar; No. 3 bile salt and crystal violet inhibit Gram-positive bacteria, potassiumtellurite inhibits non-o157 Escherichia coli, and cefixime inhibits Proteus; neutral red is a pH indicator; and agar is the coagulant of the culture medium.

Formulation

Ingredients	/liter
Enzymatic digest of casein	17.0g
Enzymatic digest of animal tissue	3.0g
Sorbitol	10.0g
Bile salt No.3	1.5g
Sodium chloride	5.0g
Neutral red	0.03g
Crystal violet	0.001g
Agar	15.0g
pH 7.1±0.2 at 25°C

Preparation

Weigh 51.5g of this product, add 1L of distilled water or deionized water, stir, heat and boil until completely dissolved, dispense into Erlenmeyer flasks, and sterilize at 121℃ for 15min. Melt the sterilized culture medium and cool it to about 45℃, add 1 tube of supporting reagent (SR0240) (0.25mg potassium tellurite and 0.005mg Cefixime) to every 100mL of basal culture medium, mix and pour into the plate.

Quality Control

Cultural characteristics observed after incubation at 35-37°C for 18-24hours

Quality control strains	Growth	Colony color
Escherichia coli ATCC25922	PR≥0.7	pale pink to red
Escherichia coli 0157:H7 NCTC12900	PR≥0.7	colorless
Staphylococcus aureus ATCC 25923	Total inhibition	–

Sorage and Shelf Life

Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.

Precautions

1. When weighing the dehydrated medium, please wear masks to avoid causing respiratory system discomfort

2. Keep container tightly closed after using to prevent clumping.

Waste Disposal

Microbiological contamination was disposed by autoclaving at 121°C for 30 minutes.

Intended Use For the isolation and differentiation of sorbitol-negative Escherichia coli serotype O157 from food and animal feed and other materials Principle and Interpretation Enzymatic ……

• R-RPE is manufactured from a red algae
• Protein based fluorescent particles specifically desinged for lateral flow applications
• Highly absorptive fluorescent molecule with excellent detectability

Description

R-Phycoerthyrin (R-RPE) Streptavidin Conjugate designed for Lateral Flow

Our Streptavidin R-Phycoerythrin conjugates are manufactured from a red algae and the first of its kind protein based fluorescent particles specifically desinged for lateral flow applications. R-RPE is a highly absorptive fluorescent molecule that has excellent detectability. It is the fluorochrome of choice when the brightest signal is needed and is therefore used most often when high sensitivity is essential for detectability and/or accuracy.

Consistent lot-to-lot performance resulting from continuous culture of source organisms and high purity.
– Very high water solubility
– Homogeneous structure with defined molecular weights
– Total control on growth conditions and nutrition, which avoids contamination from extraneous organisms and waste found in the open ocean. Proteins are harvested at the optimal stage of the growth cycle to assure uniform product characteristics. The pigment is extracted and stabilized within minutes of harvest, virtually eliminating risks from the action of proteases.

250ul stock ready to use for lateral flow

50mL lateral flow running buffer designed for Streptavidin R-Phycoerythrin particles 

• Excitation max: 566nm

• Emission max: 575nm

R-RPE is manufactured from a red algae

Protein based fluorescent particles specifically desinged for lateral flow applications

Highly absorptive fluorescent molecule with excellent detectability

Propargyl-PEG4-methane is a crosslinking reagent that can react with azide-bearing compounds or biomolecules in copper catalyzed Click Chemistry to yield a stable triazole linkage. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Propargyl-PEG4-methane is a crosslinking reagent that can react with azide-bearing compounds or biomolecules in copper catalyzed Click Chemistry to yield a stable triazole linkage. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.