Sulfo DBCO-Amine can be used to derivatize carboxyl-containing molecules or activated esters (e.g. The NHS ester) with DBCO moiety through a stable amide bond. The low mass weight will add minimal spacer to modified molecules and the hydrophilic sulfonated spacer arm will greatly improve water solubility of DBCO derivatized molecules. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Sulfo DBCO-Amine can be used to derivatize carboxyl-containing molecules or activated esters (e.g. The NHS ester) with DBCO moiety through a stable amide bond. The low mass weight will add minimal spacer to modified molecules and the hydrophilic sulfonated spacer arm will greatly improve water solubility of DBCO derivatized molecules. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
The Cylindrospermopsin plate kit is a competitive enzyme-labeled immunoassay. The Cylindrospermopsin sample extract and calibrators are pipetted into the test wells followed by the Cylindrospermopsin antibody into the test wells to initiate the reaction. During the 30 minutes incubation period, Cylin-drospermopsin from the sample and Cylindrospermopsin antigen compete for binding to the Cylindrosper-mopsin antibody. The Cylindrospermopsin antibody is captured on the walls of the test well. Following this 30-minute incubation, the contents of the wells are removed and the wells are washed to remove any unbound Cylindrospermopsin and free Cylindrospermopsin antibody. After wash, 1X HRP-conjugated Antibody#2 is added for 30 minutes incubation. The wells are washed afterwards, and a clear substrate is then added to the wells and any bound enzyme conjugate causes the conversion to a blue color. Following a 15-minute incubation, the reaction is stopped and the amount of color in each well is read. The color of the unknown samples is compared to the color of the calibrators and the Cylindrospermopsin concentration of the samples is derived.
Format:
EPA 10-Day drinking water Health Advisories for Cylindrospermopsin:
Do not Drink – 0.7 μg/L for bottle fed infants and preschool children, pregnant and nursing woman, elderly immunocompromised and liver conditions.
Do not Drink – 3.0 μg/L for school age children to adults.
Do Not Use – 20 μg/L
EPA Draft Human Health Recreational Ambient Water Quality Criteria to protect human health: 8 μg/L.
Format: 96-well microtiter plate (12 test strips of 8 wells)
Standards: 0 | 0.03 | 0.10 | 0.2 | 2 ppb
Incubation Time: 45 Minutes
Compatible for use with US EPA Method 546
Norgen’s EXTRAClean Plasma/Serum Exosome and Free-Circulating RNA Isolation Kit constitutes an all-in-one system for the purification of exosomes and the sequential isolation of RNA and free-circulating RNA from different plasma/serum sample volumes ranging from 50 μL and up to 10 mL. The purification is based on spin column chromatography that employs Norgen’s proprietary resin. The EXTRAClean columns undergo stringent processing and rigorous quality control measures to minimize contamination traces, ensuring optimal results for sensitive applications such as NGS. The kit is designed to isolate all sizes of RNA, including microRNA. The kit provides a clear advantage over other available kits in that they do not require any special instrumentation, protein precipitation reagents, extension tubes, phenol/chloroform or any protease treatments. Moreover, the kit allows the user to elute into a flexible elution volume ranging from 50 μL to 100 μL. The RNA isolated from the purified exosomes is free from any protein-bound circulating RNA and is of the highest integrity. Moreover, the free-circulating, protein-bound, RNA is free from any exosomal RNA. The purified RNA can be used in a number of downstream applications including real time PCR, NGS application, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.
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| Kit Specifications (Spin Column) | |
| Minimum Plasma Input | 4 mL |
| Maximum Plasma Input | 10 mL |
| Size of RNA Purified | All sizes, including miRNA and small RNA (< 200 nt) |
| Elution Volume | 50-100 μL |
| Time to Complete 10 Purifications | 40-45 minutes |
| Average Yields | Variable depending on specimen |
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.
Attogene’s Organophosphate detection kit is in designed specifically to detect Organophosphate in liquid samples.
Organophosphate compounds (OP) account for the largest class of rural and urban poisons in the world that are used to kill pests but can also be toxic to humans. OPs cause toxicity by means of blocking the acetylcholinesterase enzyme (AchE). The AChE-directed OPs react with a serine residue that is located at the catalytic site found within the AChE gorge. The OP targeted enzyme is no longer able to hydrolyze ACh, resulting in the buildup of ACh in the nerve synapse. This effect causes excessive excitation of the nerves, producing uncoordinated movements, tremors, paralysis and death. Both synthetic and natural(Guanitoxin) organophosphates are dangerous to humans — exposure can lead to visual, coordination, muscular, and neurological deficiencies, and in some cases even to death. In turn, exposure to OP is a significant public health concern which would significantly benefit from an improved detection platform.
Attogene’s Organophosphate detection kit is in designed specifically to detect Organophosphate in liquid samples. For solid samples a simple sample preparation method is performed. The ability to detect Organophosphate is performed is simple and sensitive. The reaction uses a chromophore that can be detected by eye. In the presence of Organophosphate, the rate of chromophore production is reduced in a concentration dependent fashion. The higher the concentration of Organophosphate the less color is produced.
Attogene’s Organophosphate detection kit is in designed specifically to detect Organophosphate in liquid samples.
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