Sulfo DBCO-PEG3-acid

Overview

• Isolate all sizes of RNA, including microRNA, irrespective of size or GC content, without bias.
• ​Versatile sample input ranges
• Isolate all sizes of free-circulating RNA, including microRNA
• The purified exosomal RNA is free from any circulating RNA-binding proteins
• No phenol extractions, Proteinase K treatment, nor carrier RNA
• No time-consuming ultracentrifugation, filtration nor special syringes are required
• No precipitation reagents, nor overnight incubation required
• Concentrate isolated exosomal RNA and are free-circulating RNA into a flexible elution volume ranging from 50 µL to 100 µL
• Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

These kits provide a fast, reliable and convenient method to sequentially isolate and concentrate exosomal RNA as well as Free-Circulating RNA from different plasma/serum sample volumes. The purification is based on spin column chromatography that employs Norgen’s proprietary resin. These kits are designed to isolate all sizes of RNA, including microRNA as well as all sizes of the free-circulating protein-bound RNA, including microRNA. These kits provide a clear advantage over other available kits in that they do not require any special instrumentation, protein precipitation reagents, extension tubes, phenol/chloroform or protease treatments. Moreover, these kits allow the user to elute into a flexible elution volume ranging from 50 µL to 100 µL. The RNA isolated from the purified exosomes is free from any protein-bound circulating RNA and is of the highest integrity. Moreover, the free-circulating, protein-bound, RNA is free from any exosomal RNA. The purified RNA can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.

Plasma/Serum Exosome and Free-Circulating RNA Isolation Mini Kit

For sample volumes ranging from 50 µL to 1 mL.

Plasma/Serum Exosome and Free-Circulating RNA Isolation Midi Kit

For sample volumes ranging from 1 mL to 4 mL.

Plasma/Serum Exosome and Free-Circulating RNA Isolation Maxi Kit

For sample volumes ranging from 4 mL to 10 mL.

Details

Supporting Data

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Kit Specifications
Minimum Plasma Input	4 mL
Maximum Plasma Input	10 mL
Size of RNA Purified	All sizes, including miRNA and small RNA (< 200 nt)
Elution Volume	50 – 100 µL
Time to Complete 10 Purifications	40 – 45 minutes
Average Yields*	Variable depending on specimen

*Please check page 5 of the product insert for the average yields and the common RNA quantification methods

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.

Important Note
This kit is suitable for the purification of exosomes from fresh or frozen serum or plasma prepared from blood collected on either EDTA or Citrate. Plasma samples prepared from blood collected on heparin should not be used as heparin can significantly interfere with many downstream applications such as RT-PCR.

Component	Cat. 59500 (50 preps)	Cat. 59600 (25 preps)	Cat. 59700 (15 preps)
Slurry E	12.5 mL	12.5 mL	12.5 mL
ExoC Buffer	8 mL	8 mL	2 x 8 mL
ExoR Buffer	12 mL	12 mL	12 mL
Lysis Buffer A	2 x 20 mL	2 x 20 mL	2 x 20 mL
Lysis Additive B	2 mL	2 mL	2 mL
Wash Solution A	2 x 18 mL	18 mL	18 mL
Elution Solution A	2 x 6 mL	6 mL	6 mL
Mini Filter Spin Columns	50	25	15
Mini Spin Columns	100	50	30
Collection Tubes	100	50	30
Elution Tubes (1.7 mL)	100	50	30
Product Insert	1	1	1

DL6MB Technical Parameter：

Max. Speed	6000rpm
Max. RCF	6600×g
Max. Capacity	6×1000ml
Timer	1~9h59min
RPM/RCF Convert	Yes
Noise (dB)	≤ 60
Temperature Range	-20~40℃
Acc/Dec	10 Kinds
Speed Accuracy	±20r/min
Temperature Accuracy	±1℃
Voltage(V/Hz)	AC 220V  50HZ/60HZ
Size (L x W x Hmm)	800×740×930mm
Net Weight(Kg)	310KG
Certificates	CE,ISO & Calibration report are available

Matched Rotors for DL6MB

Order No.	Rotor	Max Speed (rpm)	Max Volume(ml)	Max.RCF（×g）
6MB-1	Swing Rotor	4200	6×1000ml	5100
6MB-2	Angle Rotor	6000	4×300ml	5390
6MB-3	Angle Rotor	6000	6×300ml	5660
6MB-4	Angle Rotor	6000	6×500ml	6600

Features

1. Widely used in the field of blood bank, pharmaceutical factory and laboratory.

2. Frequency conversion motor, in great torque, free maintenance, no powder pollution, quick in speed up and down.

3. With pre-cooling function,Digital display which indicates the program, speed, time,RCF, temperature.

4. Micro computer control, there are 10 kinds of program and 10 kinds of acceleration and deceleration for your choice.

5. There are several kinds of rotors for your choice.

6. Electric lid lock, compact design, super speed and imbalance protection.

7. The centrifuge body is made of high-quality steel, safe and reliable.

Description

Ready to Use CdSe/ZnS Qdots Nanoparticle Streptavidin Conjugate for Lateral Flow

Our Streptavidin CdSe/ZnS Quantum Dots (QDs) conjugate is manufactured using special conjugation technology and functonally tested by lateral flow. We have coated our high quality nanoparticles with a proprietary surface coat that covalently binds the biotin forming ultra stable conjugates. The resulting Streptavidin CdSe/ZnS QDs  can irreversibly bind biotin.

fluorescent broad range UV light excitation range of 100nm to 400nm, 655nm emission

Highly stable and uniform Streptavidin CdSe/ZnS Qdots in a Ready To Use format