Sulfo DBCO-PEG3-acid is an analog of DBCO-Acid with PEG linker and a DBCO group. The DBCO groups is commonly used for copper-free Click Chemistry reactions due to its strain promoted high energy. The hydrophilic PEG chain and sulfo group increase water solubility. The terminal carboxylic acid can react with primary amine groups in the presence of activators (e.g. EDC, or HATU) to form a stable amide bond. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Detail
Sulfo DBCO-PEG3-acid is an analog of DBCO-Acid with PEG linker and a DBCO group. The DBCO groups is commonly used for copper-free Click Chemistry reactions due to its strain promoted high energy. The hydrophilic PEG chain and sulfo group increase water solubility. The terminal carboxylic acid can react with primary amine groups in the presence of activators (e.g. EDC, or HATU) to form a stable amide bond. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Other Products
Propargyl-PEG3-bromide
Product Info
Document
Product Info
Propargyl-PEG3-bromide comprises a propargyl group and a bromide group. The propargyl group reacts with azide-bearing compounds or biomolecules in Click Chemistry to yield a stable triazole linkage; copper will be needed as a catalyst. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Document
Propargyl-PEG3-bromide comprises a propargyl group and a bromide group. The propargyl group reacts with azide-bearing compounds or biomolecules in Click Chemistry to yield a stable triazole linkage; copper will be needed as a catalyst. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
170.5 mL of prepared reagent (e.g. 550 assays of 0.31 mL)
Content:
170.5 mL of prepared reagent (e.g. 550 assays of 0.31 mL)
Shipping Temperature:
Ambient
Storage Temperature:
Short term stability: 2-8oC, Long term stability: See individual component labels
Stability:
> 2 years under recommended storage conditions
Analyte:
Acetic Acid
Assay Format:
Auto-analyser
Detection Method:
Absorbance
Wavelength (nm):
340
Signal Response:
Decrease
Linear Range:
up to 1.8 g/L of acetic acid per assay
Limit of Detection:
10 mg/L (recommended assay format)
Reaction Time (min):
~ 10 min
Application examples:
Wine, beer, fruit and fruit juices, soft drinks, vinegar, vegetables, pickles, dairy products (e.g. cheese), meat, fish, bread, bakery products (and baking agents), ketchup, soy sauce, mayonnaise, dressings, paper (and cardboard), tea, pharmaceuticals (e.g. infusion solutions), feed and other materials (e.g. biological cultures, samples, etc.).
Method recognition:
Improved method
The Acetic Acid analyser format test kit is suitable for the specific measurement and analysis of acetic acid (acetate) in beverages and food products. On calibration, the prepared reagent is linear to > 28 micrograms of acetic acid per mL of assay solution.
View our complete list of organic acid assay kits.
Advantages
Very stable reagent when prepared for auto-analyser applications (> 7 days at 4oC)
PVP incorporated to prevent tannin inhibition
Linear calibration (R2 ~ 0.9995) up to 30 μg/mL of acetic acid in final reaction solution
Validated by the University of Wine, Suze la Rousse, France
Very rapid reaction
Very competitive price (cost per mL of reagent)
All reagents stable for > 2 years
Extended cofactors stability
Document
The Acetic Acid analyser format test kit is suitable for the specific measurement and analysis of acetic acid (acetate) in beverages and food products. On calibration, the prepared reagent is linear to > 28 micrograms of acetic acid per mL of assay solution.
This product provides fast and easy methods for purification of total DNA for reliable PCR and southern blotting. Total DNA can be purified from bacterial culture, body fluids, food and fermentation.
Details
Specifications
Features
Specifications
Main Functions
Isolation bacterial DNA from cultures, food and other samples
Applications
PCR, southern blot and enzyme digestion, etc.
Purification method
Mini spin column
Purification technology
Silica technology
Process method
Manual (centrifugation or vacuum)
Sample type
Culture medium, swab, parasitic blood, tissue,sputum, etc.
Sample amount
Bacterial culture medium: 0.5-2 mlTissue samples: 50-100 mg Whole blood / cell suspension: 0.5-1 ml Viscous secretion: 0.1-1 g
Elution volume
≥30μl
Time per run
≤40 minutes
Liquid carrying volume per column
800μl
Binding yield of column
100μg
Principle
This product is based on silica Column purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption column. Nucleic acid is adsorbed on the membrane, while proteinis not adsorbed and is removed with filtration. After washing proteins and other impurities, Nucleic acid was finally eluted with low-salt buffer (10mmTris, pH9.0, 0.5mm EDTA). This product carries 0.1-0.2mm acid glass beads, which can improve the wall-breaking effect of lysozyme resistant bacteria, the success rate and DNA yield through physical bead grinding.
Advantages
Fast – several samples can be extracted in 40 minutes (after digestion)
High purity – purified DNA can be directly used in various downstream applications
Good repeatability – silica technology can obtain ideal results every time
High recovery – DNA can be recovered at the level of PG
Broad spectrum – it can deal with a variety of bacteria, including Gram-positive bacteria that are difficult to disrupt
Sufficient components – this kit has carried lysozyme, protease K, RNase A and glass beads
Kit Contents
Contents
D314602
D314603
Purification Times
50 Preps
250 Preps
Hipure DNA Mini Columns I
50
2 x 125
2ml Collection Tubes
50
2 x 125
Glass Beads (0.1~0.2mm)
20 g
100 g
Buffer P1
20 ml
100 ml
Buffer DL
15 ml
80 ml
Buffer GW1
22 ml
88 ml
Buffer GW2
12 ml
50 ml
Lysozyme
60 mg
300 mg
Proteinase K
24 mg
120 mg
Protease Dissolve Buffer
5 ml
15 ml
Buffer AE
15 ml
60 ml
Storage and Stability
Lysozyme and Proteinase K should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
Document
This product provides fast and easy methods for purification of total DNA for reliable PCR and southern blotting. Total DNA can be purified from bacterial culture, body fluids, food and fermentation.