Sulfo DBCO-PEG4-Amine is a water-soluble carboxyl-reactive building block with extended PEG spacer arm. In the presence of of activators (e.g. EDC, or HATU), this reagent can be used to derivatize carboxyl groups or activated esters (e.g. The NHS ester) through a stable amide bond. The hydrophilic sulfonated spacer arm greatly improves water solubility of DBCO derivatized molecules and a provides a long and flexible connection. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Detail
Sulfo DBCO-PEG4-Amine is a water-soluble carboxyl-reactive building block with extended PEG spacer arm. In the presence of of activators (e.g. EDC, or HATU), this reagent can be used to derivatize carboxyl groups or activated esters (e.g. The NHS ester) through a stable amide bond. The hydrophilic sulfonated spacer arm greatly improves water solubility of DBCO derivatized molecules and a provides a long and flexible connection. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
African Swine Fever Virus (ASFV) is a widespread disease which infects members of the pig family(Suidae). Anumberoftick species are believed to be the vector for the disease,as well as being transmitted by raw pork and pig excrement [1]. After firstly being identified in Kenya in 1921, ASFV became endemic in sub-Saharan Africa, with regular outbreaks being reported across Europe, Asia and South America throughout the century [2]. More recently the virus was introduced in Georgia and spread throughout the region, as well as mass outbreaks occurring in China in 2018 [3]. ASFVistheonlymemberoftheAsfaridaefamily.ItisalargeenvelopeddoublestrandedDNA virus of icosahedral morphology with an average diameter of 200nm and isolates contain genomes between 170-190Kbp encoding for up to 167 open reading frames [2]. The morphology of ASFV consist of several concentric domains. An inner core contains the nucleoid coated with a thick protein layered core shell, which is surrounded by an inner lipid envelope , all of which is encompassed by the capsid [2]. ASFV begins its replication cycle in the nucleus of infected cells before moving to the cytoplasm where the majority of the replication takes place [2]. Gene transcription is highly regulated, with distinct classes of mRNA identified to accumulate at early, intermediate and late transcripts of the virus [2]. The disease induces acute haemorrhagic disease within its hosts, causing high fevers and skin haemorrhages, with death often occurring within ten days of clinical symptoms appearing [4].
References: 1: The Centre for Food Security and Public Health (2015), African Swine Fever. 2: Galindo, I. and Alonso, C., 2017. African swine fever virus: a review. Viruses, 9(5), p.103. 3: Zhou, X., Li, N., Luo, Y., Liu, Y., Miao, F., Chen, T., Zhang, S., Cao, P., Li, X., Tian, K. and Qiu, H.J., 2018. Emergence of African swine fever in China, 2018. Transboundary and emerging diseases, 65(6), pp.1482-1484. 4: Gallardo, C., Ademun, A.R., Nieto, R., Nantima, N., Arias, M., Martín, E., Pelayo, V. and Bishop, R.P., 2011. Genotyping of African swine fever virus (ASFV) isolates associated with disease outbreaks in Uganda in 2007. African Journal of biotechnology, 10(17), pp.3488-3497.
Document
Exceptional value for money Rapid detection of all clinically relevant subtypes Positive copy number standard curve for quantification Highly specific detection profile High priming efficiency Broad dynamic detection range (>6 logs) Sensitive to < 100 copies of target
Accurate controls to confirm findings
Biotin-PEG12-DBCO is a biotinylating reagent comprised of DBCO, a click chemistry handle that is frequently used to conjugate with azides on target molecules, and biotin, which is useful for affinity-based applications such as pull-down assays or for ligating with streptavidin proteins. The PEG12 imparts the hydrophilicity of the compound. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Document
Biotin-PEG12-DBCO is a biotinylating reagent comprised of DBCO, a click chemistry handle that is frequently used to conjugate with azides on target molecules, and biotin, which is useful for affinity-based applications such as pull-down assays or for ligating with streptavidin proteins. The PEG12 imparts the hydrophilicity of the compound. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Efficient, leaf punch tools for quick, precise field sampling. For use with the 3CR Bio Leaf Sample Collection Kit.
About
BASIC LEAF CUTTING TOOL
The 3CR Bio Basic Leaf Cutting Tool is a lightweight, easy-to-use, low-cost punch designed for precise sampling of thin or soft materials, ideal for fieldwork. This tool is expertly designed to core, punch, and retrieve samples from thin or soft source materials such as leaves. The tool comprises a convenient, hollow plastic pen-style holder, mounted with a durable stainless steel cutting tip at one end. It features an internal push-button-operated plunger for safe and efficient sample disc ejection. The tool produces a precise ¼ inch diameter disc, making it ideal for detailed sampling tasks. For best results, clean the cutting tip between each sample extraction by coring blank filter paper or rinsing with water or ethanol. This low-cost leaf punch is lightweight, easy to use, and perfect for fieldwork.
ADVANCED LEAF CUTTING TOOL
The 3CR Bio Advanced Leaf Cutting Tool is designed to streamline and enhance your sampling process. This high-precision leaf tissue punch produces a single ¼ inch diameter disc effortlessly. It features a unique mechanism that securely holds a standard snap-top or screw-top 2 mL storage tube in a fixed position, allowing the sample to be received without any need for touching or manipulation. Ideal for fieldwork, this advanced leaf punch significantly reduces effort and time when performing multiple samplings, ensuring accuracy and efficiency in every use.
Document
Efficient, leaf punch tools for quick, precise field sampling. For use with the 3CR Bio Leaf Sample Collection Kit.
