Sulfo DBCO-PEG4-Amine is a water-soluble carboxyl-reactive building block with extended PEG spacer arm. In the presence of of activators (e.g. EDC, or HATU), this reagent can be used to derivatize carboxyl groups or activated esters (e.g. The NHS ester) through a stable amide bond. The hydrophilic sulfonated spacer arm greatly improves water solubility of DBCO derivatized molecules and a provides a long and flexible connection. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
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Sulfo DBCO-PEG4-Amine is a water-soluble carboxyl-reactive building block with extended PEG spacer arm. In the presence of of activators (e.g. EDC, or HATU), this reagent can be used to derivatize carboxyl groups or activated esters (e.g. The NHS ester) through a stable amide bond. The hydrophilic sulfonated spacer arm greatly improves water solubility of DBCO derivatized molecules and a provides a long and flexible connection. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Giardiasis is a disease of the small bowel caused by the protozoan parasite Giardia intestinalis (syn.duodenalis or lamblia). Giardia is one of the most common intestinal parasites in the world, and occurs at very high prevalence rates in places with poor water sanitation. Individuals become infected through ingesting or coming into contact with contaminated water, food or soil. It can also be spread through the faecal-oral route due to poor hygiene practices, which makes it common in day-care centers. G. intestinalis lives inside the intestines of infected humans or other animals including cats, dogs, birds, cows, beaver and deer. Symptoms of infection include diarrhea, malaise, excessive gas, bloating, nausea, diminished interest in food, possible vomiting and weight loss.
The Giardia intestinalis TaqMan RT-PCR Kit Dx is shipped on dry ice. The components of the kit should be frozen upon arrival. If one or more of the components is not frozen when the kit is received, or if any of the components have been compromised during shipment, please contact Norgen Biotek for assistance. All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 3 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.
Component
Cat. DxTM43800 (24 rxns)
MDx TaqMan 2X RT-PCR Master Mix Dx
550 µL
Giardia intestinalis Primer & Probe Mix Dx
2 x 70 µL
Giardia intestinalis Positive Control Dx – 200,000 copies/μL
Protease K is a serine protease with high enzymatic activity and wide substrate specificity. It can preferentially decompose ester and peptide bonds adjacent to the ends of hydrophobic amino acids, sulfur-containing amino acids and aromatic amino acids C, and is often used to degrade proteins to produce short peptides. It has the typical catalytic triad Asp39-His69-Ser224 characteristic of serine proteases and two Ca2+ binding sites around the active center increase its stability, allowing it to maintain high enzyme activity under a wider range of conditions.
Technical index
Appearance: Colorless to light brown liquid; Specific activity: ≥800 U/mL; Protein concentration: ≥20 mg/mL
Enzymatic property
Source: Tritirachiumalbum; Category: EC 3.4.21.64; Molecular weight: 29 kDa(SDS-PAGE); Isoelectric point: 7.81; The optimal pH: 7.0-12.0 has high activity; Optimum temperature: 65℃; pH stability: pH 4.5-12.5(25℃,16 h); Thermal stability: stable below 50℃ (pH 8.0,30 min); Storage stability: 25℃ a year activity of more than 90% activator SDS, urea; Inhibitors: diisopropyl fluorophosphate (DFIP), benzoyl fluoride (PMSF); Storage conditions: 2-8℃, valid for 24 months.
use
Genetic diagnostic kit;
RNA and DNA extraction kit;
Extraction of non-protein components from tissues, degradation of protein impurities, such as DNA vaccine and heparin preparation;
Preparation of chromosome DNA by pulsed electrophoresis;
Western blot;
Research and development and mass production of enzymatic glycosylated albumin reagents for in vitro diagnosis.
Matters needing attention
Wear protective gloves and goggles when using, and keep well ventilated after use. This product may cause allergic skin reactions.
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Note: Price not include shipment & duty, contact us to get full quote.
An adapter for Cerillo’s Stratus plate reader. This adapter allows the Stratus plate reader to fit perfectly on the deck of the Opentrons Flex or OT-2 to allow for pipetting directly into the reader.
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An adapter for Cerillo’s Stratus plate reader. This adapter allows the Stratus plate reader to fit perfectly on the deck of the Opentrons Flex or OT-2 to allow for pipetting directly into the reader.
