Sulfo DBCO-PEG4-Maleimide is a water-soluble click chemistry reagent with a maleimide group and a DBCO moiety. The hydrophilic PEG spacer arm improves solubility in aqueous buffers. Maleimide group specifically and efficiently reacts with thiols to form stable thioether bonds. The low mass weight will add minimal spacer to modified molecules and will enable simple and efficient incorporation of DBCO moiety onto cysteine-containing peptides or other thiol-containing biomolecules. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
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Sulfo DBCO-PEG4-Maleimide is a water-soluble click chemistry reagent with a maleimide group and a DBCO moiety. The hydrophilic PEG spacer arm improves solubility in aqueous buffers. Maleimide group specifically and efficiently reacts with thiols to form stable thioether bonds. The low mass weight will add minimal spacer to modified molecules and will enable simple and efficient incorporation of DBCO moiety onto cysteine-containing peptides or other thiol-containing biomolecules. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Other Products
m-PEG17-propargyl
Product Info
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Product Info
Propargyl-PEG17-methane has an alkyne group which can participate in Click Chemistry reactions with azide compounds to yield a stable triazole linkage; copper is required as a catalyst. The hydrophilic PEG units increases solubility of the molecules in aqueous environment. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
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Propargyl-PEG17-methane has an alkyne group which can participate in Click Chemistry reactions with azide compounds to yield a stable triazole linkage; copper is required as a catalyst. The hydrophilic PEG units increases solubility of the molecules in aqueous environment. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.
The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit.
Details of the target and priming specificity are included in the individual handbooks above.
Packaged, optimised and ready to use. Expect Better Data.
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Exceptional value for money
Rapid detection of all clinically relevant subtypes
Positive copy number standard curve for quantification
Highly specific detection profile
High priming efficiency
Broad dynamic detection range (>6 logs)
Sensitive to < 100 copies of target
Accurate controls to confirm findings
Optimized for low input RNA, especially from bodily fluids such as plasma or serum at 1ng of RNA
Simple and quick workflow: library could be prepared in less than 5 hours
No gel purification for selected types of samples
Protocol optimized for RNA isolated from different types of input, including liquid biopsies (blood, plasma, serum and urine)
Complements Norgen’s Best-in-Class Total RNA (including microRNA) Purification Technology
The Small RNA Library Prep Kit for Illumina consists of all the reagents and components required to generate small RNA libraries to be used for next-generation sequencing on an Illumina platform. All molecular reagents including adaptors, primers, enzyme mixes and buffers are provided. A purification module is also provided for rapid purification of nucleic acid products generated at various steps of the workflow. The purification module utilizes Norgen’s patent resin technology which enhances recovery of desired library intermediates or final products. The library prep workflow could be used for different forms of input including purified total RNA or enriched small RNA, as well as RNA from low content inputs such as plasma, serum and urine.
Storage Conditions and Product Stability Some components require storage at -20°C, 4°C or room temperature. See individual components and box labels for storage conditions.
Step
Component
Cat. 64600 (24 preps)
3′ AdaptorLigation to Template RNA
3′ Adaptor
30 µL
3′ Adaptor Ligation Master Mix
320 µL
T4 RNA Ligase 2 (Truncated)
35 µL
5′ Adaptor Ligation
5′ Adaptor
30 µL
5′ Adaptor Ligation Master Mix
320 µL
T4 RNA Ligase 1
35 µL
cDNA Synthesis from Ligated RNA Product
Reverse Primer
30 µL
cDNA Synthesis Master Mix
220 µL
TruScript ReverseTranscriptase
35 µL
PCR Amplification
2x NGS PCR Master Mix
1.32 µL
PCR Reverse Primer
81 µL
Forward Index Primer
Included in Small RNA Library Prep Forward Index Primers (# 64640 or # 64610)
