Sulfo DBCO-TFP Ester is a water-soluble, amine-reactive labeling reagent that enables simple and efficient incorporation of Sulfo DBCO moiety onto amine-containing molecules. The hydrophilic, sulfonated spacer arm greatly improves water solubility of DBCO derivatized molecules, in many cases making them completely soluble in aqueous media. A short spacer arm adds minimal mass to modified molecules.
Sulfo DBCO-TFP Ester is a water-soluble, amine-reactive labeling reagent that enables simple and efficient incorporation of Sulfo DBCO moiety onto amine-containing molecules. The hydrophilic, sulfonated spacer arm greatly improves water solubility of DBCO derivatized molecules, in many cases making them completely soluble in aqueous media. A short spacer arm adds minimal mass to modified molecules.
LyoCakes are ready-to-use, freeze-dried master mixes pre-aliquoted PCR-strips or reaction-tubes.
LyoCakes contain: DNA polymerase(s), reaction buffer, dNTPs and primers and probes. They are rehydrated within seconds in any aqueous solutions, which makes reaction setup very easy. Simply add a biological sample and place the tube in a PCR cycler.
Storage: LyoCakes are shipped and stored simply at room-temperature. This provides a more cost efficient and ecological distribution compared to other master mixes.
As this is a customized product, we take your requirements very serious. You would like to know more? Please get in touch!
LyoCakes are ready-to-use, freeze-dried master mixes pre-aliquoted PCR-strips or reaction-tubes.
LyoCakes contain: DNA polymerase(s), reaction buffer, dNTPs and primers and probes. They are rehydrated within seconds in any aqueous solutions, which makes reaction setup very easy. Simply add a biological sample and place the tube in a PCR cycler.
Norgen’s FFPE RNA Purification Kits provide a rapid method for the isolation and purification of total RNA (including microRNA) from formalin-fixed paraffin-embedded (FFPE) tissue samples in as little as 1 hour. Using formalin to fix tissues leads to crosslinking of the RNA and proteins, and the process of embedding the tissue samples can also lead to fragmentation of the RNA over time. Norgen’s FFPE RNA Purification Kits provide conditions that allow for the partial reversing of the formalin modifications, resulting in a high quality and yield of RNA. These kits are able to purify all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA), depending on the age of the FFPE tissue as fragmentation of the RNA is known to occur over time. The RNA is preferentially purified from other cellular components without the use of phenol or chloroform.
FFPE RNA Purification Kit (Spin Column)
Maximum loading volume of 650 μL per column, and a maximum binding capacity of 50 μg per column.
FFPE RNA Purification 96-Well Kit (High Throughput)
Purification is based on 96-well column chromatography using Norgen’s proprietary resin as the separation matrix. Purification can be performed using either a vacuum manifold or centrifugation. Maximum loading volume of 400 μL per well, and a maximum binding capacity of 50 μg per well.
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| Kit Specifications | |
| Maximum Column Binding Capacity | Up to 50 µg RNA |
| Maximum Loading Volume Per Spin Column | 650 µL |
| Size of RNA Purified | All sizes, including small RNA (< 200 nt) |
| Time to Complete 10 Purifications | 1-4 hours* |
| Maximum Amount of Starting Material | 5 slices of < 20 µm thick paraffin slices 25 mg of unsectioned block |
| Average Yield | Variable due to age of paraffin blocks ~2-3 µg of Total RNA per 1 mg of fresh FFPE hamster kidney |
* Time required for purification varies by length of Proteinase K incubation and formalin crosslink-reversal
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. The DNAse I and Proteinase K should be stored at -20°C upon arrival. This kit is stable for 1 year from the date of shipment.
| Component | Cat. 25300 (50 preps) | Cat. 25400 (2 x 96 preps) |
|---|---|---|
| Digestion Buffer A | 25 mL | 2 x 25 mL |
| Buffer RL | 30 mL | 2 x 30 mL |
| Enzyme Incubation Buffer | 6 mL | 2 x 6 mL |
| Wash Solution A | 38 mL | 2 x 38 mL |
| Elution Solution A | 6 mL | 2 x 20 mL |
| Proteinase K | 12 mg | 2 x 20 mg |
| DNase I | 1 vial | 2 x 500μL |
| Micro Spin Columns | 50 | – |
| 96-Well Incubation Plate | – | 2 |
| 96-Well Plate | – | 2 |
| Adhesive Tape | – | 8 |
| Collection Tubes | 50 | – |
| 96-Well Collection Plate | – | 2 |
| Elution Tubes (1.7 mL) | 50 | – |
| 96-Well Elution Plate | – | 2 |
| Product Insert | 1 | 1 |
Cell Culture Dish 100mm
Cell Culture Dish provide Excellent surface smoothness gives clear microscopic observation
Specifications: 35mm. 60mm.100mm.150mm
PRODUCT FEATURES
For adherent cell culture: Initial adherence and proliferative property of cells via hydrophilic surface treatment.
For suspension cell culture: The surface is resistant to cell adherence, which minimizes damage or loss of cell.
Gamma radiation sterilization
Cell Culture Dish provide Excellent surface smoothness gives clear microscopic observation
Specifications: 35mm. 60mm.100mm.150mm
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