Sulfo DBCO-TFP Ester is a water-soluble, amine-reactive labeling reagent that enables simple and efficient incorporation of Sulfo DBCO moiety onto amine-containing molecules. The hydrophilic, sulfonated spacer arm greatly improves water solubility of DBCO derivatized molecules, in many cases making them completely soluble in aqueous media. A short spacer arm adds minimal mass to modified molecules.
Sulfo DBCO-TFP Ester is a water-soluble, amine-reactive labeling reagent that enables simple and efficient incorporation of Sulfo DBCO moiety onto amine-containing molecules. The hydrophilic, sulfonated spacer arm greatly improves water solubility of DBCO derivatized molecules, in many cases making them completely soluble in aqueous media. A short spacer arm adds minimal mass to modified molecules.
This kit is designed for the rapid preparation of genomic DNA from various tissue samples, cultured cells, viruses, bodily fluids and swabs using a rapid spin column protocol. Purified DNA is of an excellent yield and quality, and is immediately ready for any downstream application including PCR, qPCR, genotyping, sequencing and more. The protocol can be completed in approximately 80 minutes (including incubation time).
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| Kit Specifications | |
| Column Binding Capacity | 25 µg |
| Average Yield:* HeLa Cells (1 x 106) Tissue (from 10 mg kidney) | 8 µg 10 µg |
| Maximum Amount of Starting Material: Animal Tissues Cultured Cells Bodily Fluids (blood, saliva) Viral Suspension | 20 mg 3 x 106 cells 150 µL 150 µL |
| Time to Complete 10 Purifications | 80 minutes |
* Yield will vary depending on the type of sample processed
Storage Conditions and Product Stability
The Proteinase K should be stored at -20°C upon arrival and after reconstitution. All other solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
| Component | Cat. 24700 (50 preps) | Cat. 24750 (100 preps) | Cat. 24770 (250 preps) |
|---|---|---|---|
| Digestion Buffer A | 25 mL | 2 x 25 mL | 5 x 25 mL |
| Buffer SK | 30 mL | 2 x 30 mL | 5 x 30 mL |
| Wash Solution A | 18 mL | 2 x 18 mL | 5 x 18 mL |
| Elution Buffer B | 30 mL | 2 x 30 mL | 5 x 30 mL |
| Proteinase K | 12 mg | 2 x 12 mg | 5 x 12 mg |
| Spin Columns | 50 | 100 | 250 |
| Collection Tubes | 50 | 100 | 250 |
| Elution Tubes (1.7 mL) | 50 | 100 | 250 |
| Product Insert | 1 | 1 | 1 |
Recombinant adeno-associated virus (AAV) vectors are highly promising tools for both in vitro and in vivo gene transfer. Norgen’s AAV Purification Kits provide fast and simple procedures for concentrating and purifying AAV vectors from cell lysate and cell culture media. Purification is based on precipitation onto Norgen Biotek’s proprietary resin. Contaminating cellular debris is largely removed from the sample via a centrifugation step, while contaminating DNA and RNA is reduced using enzymatic digestion. AAV vector purified in this manner is highly active for use in in vitro and in vivo transduction experiments.
AAV Purification Kit
Norgen’s AAV Purification Kit contains sufficient materials for 15 preparations (33.5 mL per prep of supernatant (SN) or a total of 500 mL of supernatant input). Approximately 1 mL of cell pellet can be purified per prep, up to a maximum of 15 mL of cell pellet in total for the entire kit. Up to 33X sample concentration.
AAV Purification Mini Kit
Each spin column is able to concentrate and purify AAV from 0.5-8 mL of cell pellet, cell culture media, or cells and culture media mixed together. Up to 50X sample concentration. AAV vector purified in this manner is highly active for use in in vitro transduction experiments, and is eluted into a small volume (200 µL). Preparation time for 4 samples is 1.5 hours, with 45 minutes of hands-on time.
AAV Purification Midi Kit
Each spin column is able to concentrate and purify AAV from 8 mL up to 45 mL of input consisting of cell pellet, cell culture media, or cells and culture media mixed together. Up to 50X sample concentration. AAV vector purified in this manner is highly active for use in in vitro transduction experiments, and is eluted into a small volume (1 mL). The kit may be used to purify up to 8 x 25 mL or 4 x 45 mL of samples using the included columns. Preparation time for 4 samples is approximately 2 to 2.5 hours, with 1.5 hours of hands on time.
AAV Purification Maxi Kit (Slurry Format)
Each spin column is able to concentrate and purify AAV from 45 mL to 90 mL of input consisting of cell pellet, cell culture media, or cells and culture media mixed together. Up to 200X sample concentration. AAV vector purified in this manner is highly active for use in in vitro transduction experiments, and is eluted into a small volume (1-10 mL) using the optional concentration step. The kit may be used to purify up to 1 x 900 mL samples or 10 x 45-90 mL samples using the included columns. Preparation time for 1 x 900 mL sample is approximately 2.5 to 3.5 hours, with an optional concentration step requiring an additional 30 min.
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| Kit Specifications | |
| Column Binding Capacity | At least 5 x 109 AAV particles as determined by qPCR |
| AAV Vector Serotype | Any |
| Average Recovery | > 80% |
| Input Type | Cells, media, or mixed |
| Input Volume | 0.5 mL – 8 mL |
| Minimum Elution Volume | 200 µL |
| Time to Complete Purifications | 1 – 2 hours |
Storage Conditions and Product Stability
DNAse I and RNAse A should be stored at -20°C upon arrival. Elution Buffer O should be stored tightly capped at 4°C upon arrival. All other solutions should be kept tightly sealed and stored at room temperature. Once opened, the solutions should be stored at 4°C. This kit is stable for 1 year after the date of shipment.
| Component | Cat. 66100 (15 preps) | Cat. 63200 (20 preps) | Cat. 63300 (4-8 preps) | Cat. 63250 (1-10 preps) |
|---|---|---|---|---|
| Lysis Buffer S | 5.5 mL | 5.5 mL | 5.5 mL | 20 mL |
| DNAse I | – | 2 x 25 uL | 2 x 25 uL | 210 μL |
| RNAse A | – | 60 μL | 60 μL | 240 μL |
| HL-SAN Nuclease | 102 μL | – | – | – |
| Binding Buffer A | 20 mL | 4 mL | 4 mL | 2 x 8 mL |
| Purification Solution C | 60 mL | – | – | – |
| Purification Solution D | 130 mL | – | – | – |
| Wash Solution C | 2 x 130 mL | 60 mL | 60 mL | 3 x 60 mL |
| Slurry E | 12.5 mL | – | – | 2 x 14.5 mL |
| Elution Buffer O | 66 mL | 8.5 mL | 8.5 mL | 66 mL |
| Protein Neutralizer | 4 mL | 4 mL | 4 mL | 4 mL |
| Spin Columns | – | 20 | – | – |
| Mini Spin Columns | – | 20 | – | – |
| Midi Spin Columns (grey contents) with Collection Tubes | – | – | 8 | 10 |
| Midi Spin Columns (white contents) with Collection Tubes | – | – | 8 | – |
| Maxi Spin Columns (grey contents) with Collection Tubes | – | – | – | 10 |
| Maxi Spin Columns (white contents) with Collection Tubes | – | – | – | 10 |
| Collection Tubes | – | 40 | – | – |
| Elution tubes (1.7 mL) | 50 | 20 | – | – |
| Midi Elution tubes (15 mL) | – | – | 8 | 10 |
| Maxi Elution tubes (50 mL) | – | – | – | 10 |
| Product Insert | 1 | 1 | 1 | 1 |
Norgen’s Urine Preservative is designed for the rapid preservation of DNA, RNA, microRNA and proteins from fresh urine samples. In addition, the Urine Preservative eliminates the need to immediately process or freeze samples and allows the samples to be shipped to centralized testing facilities at ambient temperatures. The components of the Urine Preservative allow samples to be stored for over 2 years at room temperature with no detected degradation of urine DNA, RNA or proteins.
Norgen’s Urine Preservative is available in 2 convenient formats:
1. Urine Preservative Single Dose Ampules
With this product Norgen’s Urine Preservative is supplied in a liquid format in single dose ampules. The user simply collects 5 – 50 mL of urine into a urine collection container and adds the contents of the Urine Preservative Single Dose (Cat# 18126). The urine and preservative are then mixed, and the urine nucleic acids and proteins are preserved at room temperature.
2. Urine Collection and Preservation Tubes
Norgen’s Urine Preservative is also available in a dried format in Norgen’s Urine Collection and Preservation Tubes. The user simply collects urine into the tubes and mixes gently until the orange preservative pellet in the tube has dissolved. Norgen’s Urine Collection and Preservation Tubes are available in 3 convenient sizes:
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Select Urine Tested with the Norgen Urine Collection and Preservation Tubes
Human
Mouse
Lynx
Wolf
Urine collected from snow
Available Sizes and Formats
Storage Conditions and Product Stability
All tubes should be kept tightly sealed and stored at room temperature (15 – 25°C) for up to 2 years without any reduction in performance.
| Kit Components | Cat. 18118 (50 tubes) | Cat. 18122 (50 tubes) | Cat. 18113 (50 tubes) | Cat. 18126 (50 tubes) | Cat. 18129 (1 cup) |
|---|---|---|---|---|---|
| Urine Collection and Preservation Tubes (5 cc) | 50 | – | – | – | – |
| Urine Collection and Preservation Tubes (15 cc) | – | 50 | – | – | – |
| Urine Collection and Preservation Tubes (50 cc) | – | – | 50 | – | – |
| Urine Preservative Single Dose | – | – | – | 50 | – |
| Urine Collection and Preservation Cup (120 cc) | – | – | – | – | 1 |
| ID Labels | 50 | – | – | 50 | – |
| Product Insert | 1 | 1 | 1 | 1 | 1 |
