Swab Collection and DNA Preservation System

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Detail

Overview

  • Collect from nasal, buccal, saliva, fecal, skin, surfaces, and more
  • Completely stabilize microbiota profiles from the point-of-collection
  • Minimize bias in data analysis: NGS (Metagenomics/Microbiome), PCR and microarrays
  • No need to immediately process samples: ship and store at ambient temperatures
  • DNA preservation at room temperature over 2 years
  • Renders sample non-infectious for safe and easy shipping
  • Compatible with DNA isolation kits and protocols: Optimized isolation procedure using Norgen’s Microbiome DNA Isolation Kit

Norgen’s Swab Collection and DNA Preservation System is designed for collection, ambient storage and transport of DNA from samples collected using a swab, including nasal, buccal, saliva, fecal, skin, surfaces, etc. The Swab Collection and DNA Preservation System contains Norgen’s Swab Preservative in a liquid format. The user simply collects the specimen using the provided swab, and then transfers the swab into the Swab Preservative. The Swab Preservative prevents the growth of Gram-negative and Gram-positive bacteria and fungi, and also inactivates viruses allowing the resulting non-infectious samples to be handled and shipped safely. In addition, the Swab Preservative eliminates the need to immediately process or freeze samples and allows the samples to be shipped to centralized testing facilities at ambient temperatures. The components of the Swab Preservative allow DNA samples to be stored at room temperature for over 2 years.

DNA Isolation from Preservative

Swab samples collected and preserved using Norgen’s Swab Collection and DNA Preservation System are compatible with most DNA isolation methods. Samples stored in the tubes have been used successfully with most of Norgen Biotek’s DNA isolation kits and reagents. An optimized isolation procedure is available as part of Norgen’s Microbiome DNA Isolation Kit (Cat. 64100).

Details

Supporting Data

Figure 1 / 4

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Figure 1.  Stability of Preserved DNA
Figure 1.  Stability of Preserved DNA
Figure 2.  High Quality Preserved DNA
Figure 3.  PCR Amplification of Preserved DNA
Figure 4. Illumina MiSeq 16s rRNA metagenomics data from saliva

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Kit Specifications
Volume of Preservative1.2 mL
Preservation TemperatureRoom Temperature
Preservation time2 years at room temperature

Shelf Life and Handling

When stored at room temperature, unused Norgen Swab Preservative Tubes are stable through to the collect-before date without any reduction in performance. Please see the device insert or tube label for collect-before date.

Kit ComponentsCat. 45690 (50 units)
Swab Collection and DNA Preservation Kit50
Individually Wrapped Swabs50
Product Insert1

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