t-Boc-aminooxy-PEG5-propargyl

Description 

The ExcelRT™ One-Step RT-qPCR kit (TaqMan, ROX) is designed for reverse transcription and quantitative real-time analysis of a specific target RNA by one-step reaction. The ExcelRT™ One-Step RT-qPCR kit (TaqMan, ROX), consisting of One-Step RT Enzyme Mix and 2X One-Step Master Mix, is a convenient kit designed for highly efficient cDNA synthesis and highly specific real-time PCR in a single tube. The One-Step RT Enzyme Mix contains a thermostable ExcelRT™ Reverse Transcriptase and a RNAok™ RNase inhibitor. Consequently, One-Step RT Enzyme Mix can reverse transcribe RNA to cDNA at a wide temperature range from 42 to 60°C and active against RNase A, RNase B and RNase C. By containing specialized hot-start Taq DNA polymerase, which greatly reduce primer-dimer formation and can be activated within 2 minutes, the 2X One-Step Master Mix features high specificity and is suitable for fast cycle program. This master mix includes ROX reference dye for normalization of each RT-qPCR assay.

Features

• Reverse transcription at wide temperature range (42-60°C)
• High specificity 
• Suitable for fast cycle program
• With ROX reference dye

Storage

Aliquot to avoid multiple freeze-thaw cycles (stable within 30 freeze-thaw cycles)

Protect from light

-20°C for 12 months

The ExcelRT™ One-Step RT-qPCR kit (TaqMan, ROX) is designed for reverse transcription and quantitative real-time analysis of a specific target RNA by one-step reaction. The ExcelRT™ One-Step RT-qPCR kit (TaqMan, ROX), consisting of One-Step RT Enzyme Mix and 2X One-Step Master Mix, is a convenient kit designed for highly efficient cDNA synthesis and highly specific real-time PCR in a single tube. The One-Step RT Enzyme Mix contains a thermostable ExcelRT™ Reverse Transcriptase and a RNAok™ RNase inhibitor. Consequently, One-Step RT Enzyme Mix can reverse transcribe RNA to cDNA at a wide temperature range from 42 to 60°C and active against RNase A, RNase B and RNase C. By containing specialized hot-start Taq DNA polymerase, which greatly reduce primer-dimer formation and can be activated within 2 minutes, the 2X One-Step Master Mix features high specificity and is suitable for fast cycle program. This master mix includes ROX reference dye for normalization of each RT-qPCR assay.

Features:

Large capacity water capture freeze dryer

Tabletop medium-sized freeze dryer

Suitable for drying medium-sized samples

Apply to drying in laboratories such as cosmetics, fermentation broth, soil, materials, etc.

product description:

9kg-12kgs water capture capacity, which can meet the needs of laboratories with large freeze-drying requirements for water capture capacity

-70°C/-90°C ultra-low temperature cold trap, which can capture lower temperature organic solvents

Unique refrigeration technology, the cooling time of the cold trap from the start of the low-temperature refrigeration system to the target value is ≤3 minutes

Direct-capture large coil cold trap, higher efficiency, better ice capture effect

Hemispherical cold trap bottom surface is more conducive to the discharge of condensed water

The cold trap and condenser coil are all Teflon anti-corrosion treatment, which can freeze-dry a large amount of ultra-low temperature organic solvents

One-click hot gas defrost, with defrost compressor protection program.

Large flat flange silicone seal, permanently no need to apply any auxiliary sealing silicone grease

High-precision ultra-wide width detection, vacuum sensor with full-range automatic temperature compensation, making vacuum display more accurate

The cavity vacuum can be read and controlled accurately, and the vacuum control accuracy is ≤0.1mbar

Removable sample shelf with dedicated holes for centrifuge tube placement, making placement and height adjustment easier

Configuration of various types of chambers and T-frames can fully satisfy users’ sample placement

Optional configuration of eutectic point detection system and data export system.

The vacuum pump and sample placement chamber can be combined at will according to user needs, making the configuration more flexible and convenient

Techinical parameter

9-12L Floor tabletop and floor standing one for lab usage

Tabletop	BD1-9	BD2-9	BD1-12	BD2-12
Floor standing	BH1-9	BH2-9	BH1-12	BH2-12
Water Capture capacity	9L		12L
Cold trap temperature	-70 C	-90℃	-70C°	-90℃
Cold trap volume	15L		20L
Freeze dried chamber	High permeability organic glass/multi manifold external valve/stainless steel chamber
Sample shelf	Stainless steel φ 260mm/485mm. 3-10 layers/optional●Electric heating/manual capping/optional
Vacuum pump	Extreme vacuum 4 * 10-4mbar, pumping capacity ≥ 200L/can be equipped with various types of pumps
Oil mist filter	Standard configuration
Vacuum pump connection pipe	Single forming stainless steel flexible pipe DN16  ISO-KF L-1.5m
anti-corrosive treatment	Cold trap, condensing coil/all equipped with PTFE anti-corrosion treatment as standard. Freeze-drying organic solvents
External valve	1-48 ports/optional
Eutectic point judgment	Support/Optional
Data audit trail	Support/Optional
Power	0.9kw	1.5kw	1kw	1.6KW
Size	390*468*448mm	480*498*487mm

Overview

• Detection kits for WNV
• CE-IVD marked version available for in vitro diagnostic use
• Available in TaqMan format for analysis

West Nile Virus (WNV) belongs to the RNA virus family of Flaviviridae. It is known to spread primarily through arthropod vectors such as mosquitoes. The virus infects mainly birds but is also reported to infect humans as well as pets such as cats and dogs. In humans, the majority of the infections caused by WNV are asymptomatic. However, some individuals (the majority of the confirmed cases) could enter a second, febrile stage with flu-like symptoms – commonly known as West Nile Fever. In a more serious stage, the disease becomes neuroinvasive, causing meningitis or encephalitis. Such severe conditions could lead to mortality. As the symptoms of WNV are very similar to other common diseases but can be fatal at a severe stage, it is important to distinguish the virus early in the diagnosis, particularly at the molecular level.

WNV TaqMan RT-PCR Kit, 100 reactions

• Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
• Specific Primer and Probe mix for the pathogen/virus/viroid of interest
• Primer and Probe mix
• Positive and negative control to confirm the integrity of the kit reagents

WNV TaqMan RT-PCR Probe/Primer Set and Controls, 100 reactions

• Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest
• Nuclease-free water
• Can be used together with Norgen’s RT-PCR Master Mix (#28113) or customer supplied master mix

Details

Supporting Data

Figure 1 / 3

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Storage Conditions and Product Stability
All kit components can be stored for 1 year after the date of production without showing any reduction in performance.

All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.

Component	Cat. TM44250 (100 preps)	Cat. TM44210 (100 preps)
MDx TaqMan 2X PCR Master Mix	2 x 700 μL	–
WNV Primer & Probe Mix	280 μL	280 μL
WNV Positive Control	150 μL	150 μL
Nuclease-Free Water (Negative Control)	1.25 mL	1.25 mL
Product Insert	1	1