t-Boc-aminooxy-PEG6-propargyl is a bifunctional linker molecule. The propargyl group can react with azide-bearing compounds or biomolecules via copper catalyzed azide-alkyne Click Chemistry to yield a stable triazole linkage. The protected aminooxy can be deprotected under mild acidic conditions and then can react with an aldehyde.. The hydrophilic PEG spacer increases solubility in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Detail
t-Boc-aminooxy-PEG6-propargyl is a bifunctional linker molecule. The propargyl group can react with azide-bearing compounds or biomolecules via copper catalyzed azide-alkyne Click Chemistry to yield a stable triazole linkage. The protected aminooxy can be deprotected under mild acidic conditions and then can react with an aldehyde.. The hydrophilic PEG spacer increases solubility in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
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024040P1 Baird-Parker Agar Base
Product Info
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Product Info
Introduction
Usages: For isolation and enumeration of coagulase-positive staphylococci in foodstuffs.
Principle: Tryptone, beef extract powder and yeast extract powder provides carbon and nitrogen sources, vitamins and growth factors; sodium pyruvate and glycine to stimulate the growth of Staphylococcus aureus; lithium chloride and potassium tellurite inhibit the non-staphylococcal microorganisms; containing lecithinase staphylococcal colonies produce degradation yolk make transparent circle, while the role of the lipase produced an opaque precipitate ring; coagulase-positive staphylococci can restore potassium tellurite and produce black colonies; agar is medium coagulant.
Formulation(per liter): Pancreatic Digest of Casein 10g Beef Extract 5g Yeast Extract 1g Sodium Pyruvate 10g Glycine 12g Lithium Chloride 5g Agar 20g Final pH7.0 ± 0.2
How to use: 1.Suspend 63g in 950ML of distilled water , stirring heated to boiling ,autoclave at 121℃ for 15 minutes. 2.Diluted and treated samples.
Quality control:
Item
The name and number of strain
Growth
Colony Color
1
Staphylococcus aureus ATCC6538
Good
black
2
Staphylococcus epidermidis CMCC (B) 26069
Good
Green-blue
3
Escherichia coli ATCC25922
Not growth
—
Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.
For the enumeration of enterococci in water and other liquids by the membrane filtration technique.
Principle and Interpretation
The growth of the entire accompanying Gram-negative microbial flora is inhibited by sodium azide. Enterococci reduce 2,3,5-Triphenyl tetrazolium chloride (TTC) to give a red formazan inside the bacterial cell, their colonies are thus red. Nitrogen, minerals, and amino acids are provided by the tryptose whilst yeast extract supplies vitamins. Glucose acts as the carbon source, dipotassium phosphate buffers the medium, and agar-agar is the solidifying agent.
Formulation
Ingredients
/liter
Tryptose
20.0g
Yeast extract
5.0g
Glucose
2.0g
Disodium hydrogen phosphate
4.0g
Sodium azide
0.4g
2,3,5-Triphenyl Tetrazolium chloride
0.1g
Agar
10.0g
pH 7.2±0.1 at 25°C
Preparation
Dissolve 41.5 g in 1 L of purified water. Heat in boiling water and agitate frequently until completely dissolved. Sterilize by further heat for 20 minutes in the boiling water bath.
Quality Control
Cultural characteristics observed after incubation at 34-38°C for 40-48hours
Quality control strains
Growth
Colony color
Enterococcus faecalis ATCC29212
Good growth,PR≥0.5
deep red coloured colonies
Escherichia coli ATCC25922
Total inhibition
–
Sorage and Shelf Life
Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.
Precautions
1. When weighing the dehydrated medium, please wear masks to avoid causing respiratory system discomfort
2. Keep container tightly closed after using to prevent clumping.
Waste Disposal
Microbiological contamination was disposed by autoclaving at 121°C for 30 minutes.
Document
Intended Use For the enumeration of enterococci in water and other liquids by the membrane filtration technique. Principle and Interpretation The growth of the entire accompanying Gram-neg……
Cell Culture Flask surface is very smooth based on precise molding technology and it gives clear view when examined with microscope.
Volume: 25mL75mL175mL225mL
PRODUCT FEATURES
The product is made of medical grade USP CLASS VI polymer polystyrene
The product is made under a 100,00- class dust-free manufacturing site
Two kinds of product line up are providing.
For adherent cell culture: Initial adherence and proliferative property of cells via hydrophilic surface treatment.
For suspension cell culture: The surface is resistant to cell adherence, which minimizes damage or loss of cell.
Large mouthed design makes easy operation of pipet or cell scraper. The surface of flask is uniform and smooth, hence the clear view can be obtained when microscopic observation.
The hydrophobic filter cap can prevent invasion of fungi and bacteria without water absorb.
Gamma radiation sterilization
Non-Pyrogenic, DNase/Rnase free.
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Cell Culture Flask surface is very smooth based on precise molding technology and it gives clear view when examined with microscope.