t-Boc-N-Amido-PEG6-propargyl

Overview

• Rapid spin column purification of genomic DNA from viable yeast cells, fungal spores or mycelium, and bacteria including Gram-positive
• Bead tubes (provided) allow for effective mechanical homogenization
• Purified DNA is of high quality and integrity and compatible with any sensitive downstream applications such as PCR, qPCR, RFLP and more

These kits provide fast and reliable procedures for the purification of genomic DNA from viable yeast cells, fungal spores or mycelium, and bacteria including Gram-positive. Genomic DNA is efficiently extracted from the cells by a combination of heat treatment, detergents and Bead Tubes (provided). An optional lyticase treatment allows for improved DNA yields with certain fungal and yeast species. Recovered genomic DNA is of excellent yield and purity for any downstream application including PCR, qPCR, Restriction Fragment Length Polymorphism (RFLP), Amplified Fragment Length Polymorphism (AFLP), sequencing, SNP analysis and more.

Fungi/Yeast Genomic DNA Isolation Kit (Spin Column)

This kit provides rapid spin column purification of genomic DNA from viable yeast cells, fungal spores or mycelium, and bacteria including Gram-positive. Preparation time for a single sample is less then 30 minutes, and each kit contains sufficient materials for 50 preparations.

Fungi/Yeast Genomic DNA Isolation 96-Well Kit (HT)

Norgen’s Fungi/Yeast Genomic DNA Isolation 96-Well Kit provides a fast, reliable and simple procedure for high throughput isolation of DNA from viable yeast cells, fungal spores or mycelium and Gram-positive bacteria. The purification could be performed on either a vacuum manifold or using centrifugation. Complete 96 purifications in 40 minutes.

Details

Supporting Data

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Kit Specifications
Column Binding Capacity	50 μg
Maximum Column Loading Volume	650 μL
Maximum Amount of Starting Material: Fungi (wet weight) Yeast or Bacteria Culture	50 mg 0.5mL – 1mL
Average Yield* Pichia sp. (yeast) Botrytis cinerea ​Fusarium sp. Aspergillis niger Mucor racemosus Cladosporium cladosporioides Penicillium sp. Rhizopus oryzae Alternaria tenuissima	25 μg 32 μg 42 μg 26 μg 15 μg 12 μg 40 μg 7 μg 30 μg
Time to Complete 10 purifications	30 minutes

* Yield will vary depending on the type of sample processed

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.

Component	Cat. 27300 (50 preps)	Cat. 27350 (192 preps)
Lysis Buffer L	30 mL	2 x 60 mL
Resuspension Solution A	20 mL	60 mL
Solution BX	28 mL	2 x 28 mL
Wash Solution A	18 mL	2 x 38 mL
Elution Buffer B	15 mL	30 mL
Bead Tubes	50	200
Spin Columns	50	–
Collection Tubes	50	–
96-Well Plate	–	2
96-Well Collection Plate	–	2
Adhesive Tape	–	4
Elution Tubes (1.7 mL)	50	–
96-Well Elution Plate	–	2
Product Insert	1	1

Propargyl-PEG18-alcohol is a crosslinker that can react with azide compounds under the catalyzation of copper to form stable triazole linkage. The hydrophilic PEG units increase the solubility of the molecule in aqueous environment. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Propargyl-PEG18-alcohol is a crosslinker that can react with azide compounds under the catalyzation of copper to form stable triazole linkage. The hydrophilic PEG units increase the solubility of the molecule in aqueous environment. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.