t-Boc-N-Amido-PEG6-propargyl

Introduction

Usages:

For differentiation of enterobacteriaceae.

Principle:

Peptone, beef extract powder and yeast extract powder provide nitrogen, vitamins, minerals; lactose, dextrose into fermentable sugars, which produce acid when measured by the phenol red indicator, acid yellow, basic red; thiosulfate sodium can be reduced to some bacteria to hydrogen sulfide, to produce a black iron sulfide and iron salts; sodium chloride to maintain osmotic equilibrium; agar as medium coagulant.

Formulation(per liter):

Peptone 20g

Beef extract powder 3g

Yeast extract powder 3g

Lactose 10g

Dextrose 1g

Sodium chloride 5g

Ferric ammonium citrate 0.5g

Sodium thiosulfate 0.5g

Agar 12g

Phenol red 0.05g

Final pH 7.4 ± 0.2

How to use:

1.Suspend 54.5g in 1L of distilled water , stirring heated to boiling ,autoclave at 121 for 15 minutes.

2.Diluted and treated samples.

Quality control:

Item	The name and number of strain	Growth	Colony Color
1	Escherichia coli ATCC25922	Good	A/A
2	Proteus CMCC (B) 49027	Good	K/A
3	Salmonella typhimurium CMCC (B) 50115	Good	K/A

“A”Acid , “K” Alkality

Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.

500g

About

PACE Multiplex Master Mix for the simultaneous detection of up to four targets in one reaction. Save time, cost, and consumables while maximising data generation.

PACE Multiplex Master Mix is an advanced and versatile extension of our PACE 2.0 Genotyping Master Mix, formulated for the simultaneous detection of up to four targets in one reaction well. For example, two bi-allelic SNPs, or one reference gene and a further three genes of interest.

PACE Multiplex genotyping assay designs are available from 3CR Bioscience through our free PACE assay design service; once designed, users can purchase assay primers independently or through 3CR Bioscience using our partial or full-assay validation service.

Users will require a plate reader capable of reading FAM, HEX, ATTO 590, ATTO 647N and reference dye ATTO 680 (wavelengths in the PACE Multiplex Master Mix User Guide). PACE Multiplex Master Mix is supplied at 2x concentration for convenience and with or without ATTO 680 reference dye at a range of levels to ensure compatibility with your qPCR machine or reader.

PACE Multiplex Master Mix for the simultaneous detection of up to four targets in one reaction. Save time, cost, and consumables while maximising data generation.