t-Boc-N-Amido-PEG8-propargyl has an alkyne group and Boc-protected amine. The alkyne group reacts with azide-bearing compounds or biomolecules via copper catalyzed Click Chemistry. The deprotection of Boc-protected amine can be peformed under mild acidic conditions. The hydrophilic PEG chain enhances the solubility of the molecule in aqueous solution. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Detail
t-Boc-N-Amido-PEG8-propargyl has an alkyne group and Boc-protected amine. The alkyne group reacts with azide-bearing compounds or biomolecules via copper catalyzed Click Chemistry. The deprotection of Boc-protected amine can be peformed under mild acidic conditions. The hydrophilic PEG chain enhances the solubility of the molecule in aqueous solution. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Other Products
Vibrio cholerae TaqMan PCR Detection Kits
Product Info
Document
Product Info
Overview
Detection kits for Vibrio cholerae
Available in TaqMan format for analysis
CE-IVD marked version available for in vitro diagnostic use
Vibrio cholerae is a comma-shaped, gram-negative bacterium. It is the cause of cholera in humans, which affects the upper small intestine. Transmission of the disease is mainly through contaminated food or water. Human subjects affected by cholera exhibit severe watery diarrhea and vomiting, caused by the cholera toxin produced by the bacterium. Many cases of cholera are life-threatening, as diarrhea and associated vomiting can lead to rapid dehydration and electrolyte loss. Even with the extensive research on its epidemiology, cholera still affects over 5 million people per year worldwide.
Vibrio cholerae TaqMan PCR Kit, 100 reactions
Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
Specific Primer and Probe mix for the pathogen/virus/viroid of interest
Primer and Probe mix
Positive and negative control to confirm the integrity of the kit reagents
Vibrio cholerae TaqMan PCR Probe/Primer Set and Controls, 100 reactions
Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest
Nuclease-free water
Can be used together with Norgen’s PCR Master Mix (#28007) or customer supplied master mix
Storage Conditions and Product Stability All kit components can be stored for 2 years after the date of production without showing any reduction in performance.
All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.
This kit is suitable for extracting total pathogen nucleic acid from a variety of clinical samples such as blood, serum, plasma, swab soaking solution, fluid accumulation and homogenate solution. This kit is designed to remove host cells background nucleic acid and enrich pathogen nucleic acid (including viral/bacterial/fungal DNA/RNA) from the sample. Purified DNA/RNA is ready for downstream applications such as PCR, virus detection, tNGS and other related experiments.
Details
Specifications
Features
Specifications
Main Functions
Extract Pathogen RNA/DNA from 0.5-1.5ml whole blood, plasma, serum, body fluid, homogenate suspension, culture solution, cell suspension, soaking solution or concentrate pathogen solution for tNGS application, remove host background nucleic acid.
Applications
Real Time PCR, biochip analysis, NGS
Products
Pathogen DNA / RNA
Purification method
Polydisperse magnetic beads
Purification technology
Magnetic beads technology
Process method
Manual or automatic
Sample type
whole blood, plasma, serum, body fluid, homogenate suspension, culture solution, cell suspension, soaking solution or concentrate pathogen solution
Sample amount
0.5 – 1.5 ml
Adaptive instrument
Nucleic acid extractor, pipetting workstation
Principle
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. After adding magnetic particles and binding solution, DNA/RNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA/RNA was eluted by Buffer NFW.
Kit Contents
Contents
R667200C
R667202C
Purification Times
24 Preps
96 Preps
2ml Bead Tube (0.4g)
24
96
DNase I (Powder)
10 mg
15 mg
DNase Buffer
5 ml
20 ml
Protease Dissolve Buffer
3 ml
8 ml
Lysis Buffer LBX1
40 ml
180 ml
Buffer TL
5 ml
20 ml
Proteinase K
24 mg
120 mg
MagBind Particles N9
1.2 ml
5 ml
Buffer MLB
30 ml
120 ml
Buffer MW1*
13 ml
110 ml
Buffer MW2*
10 ml
50 ml
Buffer AVE
10 ml
20 ml
Storage and Stability
Proteinase K, DNase I powder and MagPure Particles N9 should be stored at 2–8°C upon arrival. However, short-term storage (up to 8 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for at least 18 months under these conditions.
Document
This kit is suitable for extracting total pathogen nucleic acid from a variety of clinical samples such as blood, serum, plasma, swab soaking solution, fluid accumulation and homogenate solution. This kit is designed to remove host cells background nucleic acid and enrich pathogen nucleic acid (including viral/bacterial/fungal DNA/RNA) from the sample. Purified DNA/RNA is ready for downstream applications such as PCR, virus detection, tNGS and other related experiments.
Apply to drying in laboratories such as cosmetics, fermentation broth, soil, materials, etc.
product description:
9kg-12kgs water capture capacity, which can meet the needs of laboratories with large freeze-drying requirements for water capture capacity
-70°C/-90°C ultra-low temperature cold trap, which can capture lower temperature organic solvents
Unique refrigeration technology, the cooling time of the cold trap from the start of the low-temperature refrigeration system to the target value is ≤3 minutes
Hemispherical cold trap bottom surface is more conducive to the discharge of condensed water
The cold trap and condenser coil are all Teflon anti-corrosion treatment, which can freeze-dry a large amount of ultra-low temperature organic solvents
One-click hot gas defrost, with defrost compressor protection program.
Large flat flange silicone seal, permanently no need to apply any auxiliary sealing silicone grease
High-precision ultra-wide width detection, vacuum sensor with full-range automatic temperature compensation, making vacuum display more accurate
The cavity vacuum can be read and controlled accurately, and the vacuum control accuracy is ≤0.1mbar
Removable sample shelf with dedicated holes for centrifuge tube placement, making placement and height adjustment easier
Configuration of various types of chambers and T-frames can fully satisfy users’ sample placement
Optional configuration of eutectic point detection system and data export system.
The vacuum pump and sample placement chamber can be combined at will according to user needs, making the configuration more flexible and convenient
Techinical parameter
9-12L Floor tabletop and floor standing one for lab usage
Tabletop
BD1-9
BD2-9
BD1-12
BD2-12
Floor standing
BH1-9
BH2-9
BH1-12
BH2-12
Water Capture capacity
9L
12L
Cold trap temperature
-70 C
-90℃
-70C°
-90℃
Cold trap volume
15L
20L
Freeze dried chamber
High permeability organic glass/multi manifold external valve/stainless steel chamber