t-Boc-N-Amido-PEG8-propargyl has an alkyne group and Boc-protected amine. The alkyne group reacts with azide-bearing compounds or biomolecules via copper catalyzed Click Chemistry. The deprotection of Boc-protected amine can be peformed under mild acidic conditions. The hydrophilic PEG chain enhances the solubility of the molecule in aqueous solution. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Detail
t-Boc-N-Amido-PEG8-propargyl has an alkyne group and Boc-protected amine. The alkyne group reacts with azide-bearing compounds or biomolecules via copper catalyzed Click Chemistry. The deprotection of Boc-protected amine can be peformed under mild acidic conditions. The hydrophilic PEG chain enhances the solubility of the molecule in aqueous solution. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Other Products
Propargyl-PEG5-Ms
Product Info
Document
Product Info
Propargyl-PEG5-Ms represents a bifunctional linker possessing a proparygyl group reactive towards azides in copper (I) click chemistry to form stable triazoles with the target compound as well as a mesyl group which is a good leaving group for nucleophilic reactions.
Document
Propargyl-PEG5-Ms represents a bifunctional linker possessing a proparygyl group reactive towards azides in copper (I) click chemistry to form stable triazoles with the target compound as well as a mesyl group which is a good leaving group for nucleophilic reactions.
The 16S V3-V4 Library Preparation Kit for Illumina consists of the reagents and components required for library preparation of the 16S V3-V4 amplicon libraries to be used for next-generation sequencing on Illumina platforms. All molecular reagents including primers, enzyme mixes, indexes, and buffers are provided. Instructions for PCR clean up with the AMPure XP Magnetic Beads (supplied by customer) are also included for rapid purification of nucleic acid products generated at two steps of the workflow. The library prep workflow could be used for purified DNA inputs from different sources including stool, soil, water, saliva, plant, urine, skin swab, vaginal swab, cheek swab, nasal swab, plasma/serum, tongue swab, gum swab, and others.
The 16S V3-V4 Library Preparation Kit for Illumina has a streamlined procedure that reduces the handling time such that the library prep procedure can be completed in approximately 4 hours (see diagram below). Input DNA is first subjected to targeted PCR to amplify the V3-V4 region of the DNA encoding 16S rRNA. The post-PCR reaction is then cleaned up using AMPure XP beads. Dual index primers are then added using a limited-cycle PCR. The indexed amplicons flanked by 5′ and 3′ barcoded adaptors are then cleaned using AMPure XP beads. The libraries are then ready for quantification, pooling and sequencing.
Details
Minimum amount of starting material:
2.5 µL of DNA (5 ng/µL)
Time to complete library preparation:
4 hours
Storage Conditions and Product Stability Norgen’s 16S V3-V4 Library Prep Kit for Illumina is shipped as one kit box (for the 24 prep kit) or two sub-component kits (for the 96 prep kit). All kits should be stored at -20°C upon arrival.
All kit components should remain stable for at least 1 year when stored at the specified storage conditions.
Isothermal Amplification Kit: Freeze-Dried Reagent, Stable & Easy to Operate
Product Detail
Kit Storage and Term of Validity
Storage term: stored at ≤-20℃,keep away from light, avoid heavy weight and repeated freezing and thawing.
Term of Validity: 14 months
Isothermal Nucleic Acid Principle Summary
The kit is based on rapid nucleic acid amplification technology at room temperature and constant temperature, its principle is that at room and constant temperature, the recombinase and primer form a protein/single-stranded nucleotide complex Rec/ssDNA, and invade the double-stranded DNA template with the help of auxiliary proteins and single-stranded binding protein SSB; then form a D-loop region at the invasion point and start to scan the DNA duplex, after finding the target region complementary to the primer and disintegration of the complex Rec/ssDNA, the polymerase also binds to the 3′ end of the primer to start the chain extension.
Isothermal Nucleic Acid Product Features
1/ High sensitivity and specificity, short reaction time.
2/ The reagent form is freeze-dried, stable and easy to operate.
3/ The reaction can be operated by metal bath and water bath pot without purchasing expensive PCR apparatus.
Technical Parameters:
Parameters
Details
Product Name
DNA Isothermal Amplification Kit Basic
Manufacturer
Amp-future
Storage Temperature
-20°C
Kit Components
Enzymes, Buffers ,Reagents
Packaging
48 Tests/box
Detection Limit
500-1000copies/µL
Shipping
ICE
Test Time
5-20mins
Isothermal Nucleic Acid Applications
Suitable for DNA isothermal rapid amplification kit(Basic type)
Primer: Require pair of nucleotide primers with the length of 25-35 bp.
Colloidal gold probe:Require a sequence of 46-52nt in length
DNA basic kit reaction temperature is 39 to 42℃ and time is 5-20 minutes.
Notes
1/ Please avoid nucleic acid contamination and set blank control during reaction due to the high sensitivity of the kit.
2/ Please take out the required quantity of MIRA reaction units for the experiment, and put the rest under storage conditions when performing the experiment.