t-Boc-N-Amido-PEG8-propargyl

Description

About the genesig q32 Real-Time PCR Instrument

genesig 32 Overview
The genesig q32 Real-Time PCR Instrument is one of the fastest qPCR instruments on the market today due to its rapid heating and cooling. Assembled from just a few building blocks, this robust qPCR instrument allows analysis of up to 32 samples in both tube or strip format. The powerful genesig q32 software is easily installed from a USB flash drive onto any PC or Mac. The genesig q32 is used with 550 genesig Real-Time PCR Kits for human, vet, and food pathogens, as well as food speciation testing

The genesig Real-Time PCR Kit product range includes tests for a massive range of applications:

• Human Infectious Disease Screening
• Veterinary Diagnostics
• Food and Water testing
• Biothreat Detection

genesig q32 – Cutting edge design and technology
Assembled from just a few building blocks the robust genesig q32 system is easy to transport and install. Up to 32 samples can be run in 0.1 ml tubes or 8-strip format. Fast heating and cooling is achieved by utilizing robust Peltier elements, whilst assay performance is supported further by a heated lid design. Excitation is provided by high intensity LEDs filtered to provide light at 500nm that is capable of exciting all fluorophores commonly used in qPCR. A prism is used to generate spectra from fluorescent emissions. These spectra are imaged using a CMOS camera.

genesig q32 software
The genesig q32 software is delivered and easily installed from a USB flash drive. When installed in a network, the software can control several q32 instruments connected via LAN. The powerful and easy to use software provides the following features:

• Quick start using templates for all genesig kit applications
• Straightforward setup and editing of sample and target information
• Automated analysis modules for absolute and relative quantification, melting curve analysis and endpoint genotyping
• Analysis of full spectral data
• Compatible with Windows, Mac & Linux Systems
• Instrument start from a USB flash drive, using preprogrammed settings
• Data export functions

genesig Real-Time PCR Kit compatible
genesig Real-Time PCR Kits are suitable for use on the genesig q32 instrument. The genesig Kit range includes over 550 kits for human, vet, and food pathogens, as well as food speciation testing. All genesig Kits include a positive control and resuspension buffers. The kits come freeze-dried so that they can be shipped at room temperature for fast delivery to anywhere in the World.

Automated run templates available for:

• genesig Easy Real-Time PCR Kits
• genesig Advanced Real-Time PCR Kits
• genesig Standard Real-Time PCR Kits
• snpsig Real-Time PCR Kits
• quasa Real-Time PCR Kits
• GMO Real-Time PCR Kits

Dimensions: W 24 cm x D 27 cm x H 23 cm
Weight: 7 kg
Operating noise: Electrical: Voltage: 100 – 240 V ( 10%); Frequency: 50 – 60 Hz ( 10%); Power: 170 W
Number of reactions: 32
Reaction vessels: 0.1ml tube and/or 8-tube strips
Reaction volume: 10 – 100 μl (20 μl recommended)
Temperature cycling system: Peltier-based heating & cooling from 40 to 99°C; Heating rate: 4°C/s & Cooling rate: 3°C/s
Temperature uniformity:  /- 0.05°C (SD)
Temperature accuracy:  /- 0.25°C
Run time: Fluorescence excitation: 500 nm nm (blue LED)
Fluorescence detection: 120 optical channels from 510 to 750 nm (CMOS camera)
Factory-calibrated dyes (at shipment): FAM; VIC / HEX
Applications: Relative and absolute quantification analysis; Melting peak analysis; Endpoint genotyping analysis
Sensitivity: 1.1 fold discrimination; 9-log dynamic range; Single copy detection
Connection options: LAN; Direct connection to computer (RJ45); PC-free (USB stick/flash drive controlled)Candidatus Liberibacter asiaticus Manual

Guaranteed results using 550 genesig kits
Fast DNA and RNA analysis in less than 60 minutes
Quick and easy set-up and run
Automated data analysis result calling
Small and robust instrument
Exceptional value for money

Bis-propargyl-PEG13 comprises two propargyl groups which can form triazole linkages with azide-bearing compounds or biomolecules in copper catalyzed Click Chemistry reactions. The hydrophilic PEG units help improve the solubility of the molecule in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Bis-propargyl-PEG13 comprises two propargyl groups which can form triazole linkages with azide-bearing compounds or biomolecules in copper catalyzed Click Chemistry reactions. The hydrophilic PEG units help improve the solubility of the molecule in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Gel images of different ranges of library size selection. Sheared human genomic DNA was used as input.

.

Library size selection is an enrichment of a specific range of library sizes for NGS library preparations. The NGS library preparation is related to the quality of the sequencing data. Precise NGS library size selection can increase sequencing efficiency, improve data quality, and reduce costs.

There are two types of sequencing technologies: short-read sequencing and long-read sequencing. Short-read sequencing uses DNA libraries that contain small insert DNA fragments of similar sizes, usually several hundred base pairs. The sequencing efficiency can be improved if the DNA size selection is in the right range. Cat.# 20104S and 20104L are the best kits for NGS library size selection of illumina paired-end 100 (PE100) sequencing with 100-200 bp library inserts; Cat.# 20105S and 20105L are the best kits for NGS library size selection of illumina paired-end 150 (PE150) sequencing with 150-300 bp library inserts; and Cat.# 20106S and 20106L are the best kits for NGS library size selection of illumina paired-end 300 (PE300) sequencing with 300-600 bp library inserts.

Long-read sequencing uses a large DNA fragment as input and makes very long reads. Usually, library size selection is preferred to remove smaller fragments. Cat.# 20110S and 20110L are the best kits for long-read sequencing size selection with DNA sizes >5 kb, and Cat.# 20111S and 20111L are the best kits for long-read sequencing size selection with DNA sizes >10 kb.

The magnetic beads, or SPRI (Solid Phase Reversible Immobilization) beads, is well used for the purification of DNA due to their reversible DNA binding. The NGS library can be size-selected by the magnetic beads or SPRI beads. The properties of the magnetic beads can be changed for a specific range of DNA binding. The contaminants and other unwanted components in the libraries can also be removed during size selection.

Specific ranges of NGS libraries can be selected using magnetic beads with different buffer compositions. The first DNA-beads binding step, also called the right-side clean-up, removes large NGS library fragments. The large NGS library fragments that bind to the beads are discarded with the beads pellet. The desired NGS library fragments in the supernatant are transferred to a new well, and new beads are added to the supernatant for the second beads-DNA binding, also called the left-side clean-up. After the rinsing step, the NGS library fragments with the dual selection are eluted in water or an appropriate buffer. The magnetic beads method has great advantages over time-consuming column purification and tedious gel-based purification.

NGS library size selection with dual clean-ups.

.

Library size selection for long-read sequencing only requires a single clean-up. In this case, only the large library fragments are bound to the beads, while other small library fragments are discarded with the supernatant. The selected larger library fragments are eluted in water or an appropriate buffer after the rinsing step.

NGS library size selection with single clean-up for >5 kb and >10 kb libraries.

.

Features of NGS library size selection

• • • 5 ranges for NGS library size selection
      • • illumina PE100 sequencing: 250-350 bp
        • illumina PE150 sequencing: 300-450 bp
        • illumina PE100 sequencing: 450-750 bp
        • Long-read sequencing: >5 kb
        • Long-read sequencing: >10 kb
    • Rapid protocol: only 20 minutes
    • Simple workflow
      • • No columns required
        • No gel purification required
        • No centrifugation required