t-Boc-N-amido-Tri-(propargyl-PEG10-ethoxymethyl)-methane is reactive with azide-bearing compounds or biomolecules via copper catalyzed azide-alkyne Click Chemistry to yield a stable triazole linkage. The Boc group can be deprotected under mild acidic conditions to form the free amine. Reagent grade, for research use only.
t-Boc-N-amido-Tri-(propargyl-PEG10-ethoxymethyl)-methane is reactive with azide-bearing compounds or biomolecules via copper catalyzed azide-alkyne Click Chemistry to yield a stable triazole linkage. The Boc group can be deprotected under mild acidic conditions to form the free amine. Reagent grade, for research use only.
Name of Product
Anisakidae – IgG ELISA
Catalog Number
AF 9800
Short Info
This ELISA kit is for the quantitative detection of IgG antibodies against parasites of Anisakidae family in human serum. Serology is an aid for diagnosis and cannot be used as the sole method of diagnosis.
This product is manufactured by Bordier Affinity Products in Switzerland and distributed in Germany exclusively by Milenia Biotec.
Method/Platform
ELISA in microplate format
Range/Assay Sensivity
97%
Test Principle
Specific antibodies in the sample bind to Anisakidae excreted/secreted larval antigens sensitized on microtiter plates. The presence of parasite specific antibodies is detected with a Protein A alkaline phosphatase conjugate.
Brief Instructions
Storage
2-8° C
Components
ELISA wells, dilution buffer, washing solution, control sera, conjugate, substrate solution, stopping solution
12 x 8 strips (96 tests)
This ELISA kit is for the quantitative detection of IgG antibodies against parasites of Anisakidae family in human serum. Serology is an aid for diagnosis and cannot be used as the sole method of diagnosis.
This product is manufactured by Bordier Affinity Products in Switzerland and distributed in Germany exclusively by Milenia Biotec.
This kit is suitable for extracting total pathogen nucleic acid from a variety of clinical samples (including serum and plasma). The kit is based on super paramagnetic particles purification technology. Purified DNA/RNA is ready for downstream applications such as Real Time PCR, biochip analysis, NGS and other related experiments.
Specifications
| Features | Specifications |
| Main Functions | Extract Pathogen RNA/DNA from 0.5ml plasma, serum, body fluid, homogenate suspension, culture solution, cell suspension, soaking solution or concentrate pathogen solution for mNGS application, remove host background nucleic acid. |
| Applications | RT-PCR，Real Time PCR, biochip analysis, NGS |
| Products | Pathogen DNA / RNA |
| Purification method | Polydisperse magnetic beads |
| Purification technology | Magnetic beads technology |
| Process method | Manual or automatic |
| Sample type | low/cell-free samples such as plasma, serum, body fluid, homogenate suspension, culture solution, cell suspension, soaking solution or concentrate pathogen solution |
| Sample amount | 0.5 ml |
| Adaptive instrument | Nucleic acid extractor, pipetting workstation |
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. After adding magnetic particles and binding solution, DNA/RNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA/RNA was eluted by Buffer NFW.
Kit Contents
| Contents | R667200B | R6672-02B |
| Purification Times | 24 Preps | 96 Preps |
| 2ml Bead Tubes | 24 | 96 |
| Proteinase K | 12 mg | 50 mg |
| Protease Dissolve Buffer | 1.8 ml | 3 ml |
| Buffer SDS (20%) | 1.8 ml | 8 ml |
| MagBind Particles | 0.6 ml | 2.5 ml |
| Buffer MLB | 15 ml | 60 ml |
| Buffer MW1* | 13 ml | 44 ml |
| Buffer MW2* | 6 ml | 50 ml |
| Buffer AVE | 5 ml | 30 ml |
Storage and Stability
MagBind Particles and Proteinase K Solution should be stored at 2–8°C upon arrival. However, short-term storage (up to 8 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for 18 months under these conditions.
This kit is suitable for extracting total pathogen nucleic acid from a variety of clinical samples (including serum and plasma). The kit is based on super paramagnetic particles purification technology. Purified DNA/RNA is ready for downstream applications such as Real Time PCR, biochip analysis, NGS and other related experiments.
Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.
The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit.
Details of the target and priming specificity are included in the individual handbooks above.
Packaged, optimised and ready to use. Expect Better Data.
Exceptional value for money
Rapid detection of all clinically relevant subtypes
Positive copy number standard curve for quantification
Highly specific detection profile
High priming efficiency
Broad dynamic detection range (>6 logs)
Sensitive to < 100 copies of target
Accurate controls to confirm findings
