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T4 DNA Ligase is an ATP and Mg2+ dependent dsDNA ligase which catalyses the formation of a phosphodiester bond between 3’-hydroxyl and 5’-phosphate termini in duplex DNA, duplex RNA and some DNA/RNA hybrids. T4 DNA Ligase is active on both blunt-end and cohesive-end substrates. It is also completely inactivated by incubating at 70°C for 10 minutes.
This is a high-quality (commercial grade) version of the T4 DNA Ligase. T4 DNA Ligase is recombinantly produced in E. coli. ArcticZymes’ T4 DNA Ligase is extensively tested for contaminating DNase and RNase activities as well as residual host-cell gDNA.
Key Features
- ATP and Mg2+ dependent dsDNA ligase
- Easily heat-inactivated at 70°C for 10 minutes
- Extensively tested for contaminating DNase and RNase activities as well
as residual host-cell gDNA
Applications
- Ligation of dsDNA
- NGS library prep
- Molecular cloning
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