T7 RNA Polymerase catalyzes the formation of RNA from a DNA template in the 5’-3’ direction and is commonly used in in vitro transcription (IVT) applications. The enzyme is T7 promoter specific, requires Mg2+ as a cofactor and can use modified nucleotides for the synthesis. The T7 RNA Polymerase requires a double-stranded DNA template and can produce full-length RNA transcripts.
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T7 RNA Polymerase catalyzes the formation of RNA from a DNA template in the 5’-3’ direction and is commonly used in in vitro transcription (IVT) applications. The enzyme is T7 promoter specific, requires Mg2+ as a cofactor and can use modified nucleotides for the synthesis. The T7 RNA Polymerase requires a double-stranded DNA template and can produce full-length RNA transcripts.
Key Features
T7 promoter-specific RNA polymerase
Available in standard glycerol-based formulation as well as lyophilization-friendly formulation without glycerol.
Suggested Applications
In vitro transcription of RNA
Molecular diagnostics (NASBA and other)
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Properties
Quality Control
ArcticZymes is dedicated to the quality of our products. T7 RNA Polymerase is manufactured at our ISO 13485 certified facility in Norway.
Other Products
Human Enterovirus (HEV) TaqMan RT-PCR Detection Kits
Product Info
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Product Info
Overview
Detection kits for HEV
CE-IVD marked version available for in vitro diagnostic use
Available in TaqMan format for analysis
The Enteroviruses are a genus of the family Picornaviridae, and are a large and diverse group of small RNA viruses characterized by a ssRNA molecule of positive sense. To date, over 70 serotypes of Enterovirus have been isolated from man. The human enteroviruses include poliovirus, Coxsackie A and Coxsackie B viruses, enterocytopathic human orphan (ECHO) viruses and other enteroviruses. Enteroviruses infect millions of people worldwide each year, and the infection can result in a wide variety of symptoms depending on the serotype of the enterovirus. Symptoms of infection may include, but are not limited to, mild respiratory illness (common cold), hand, foot and mouth disease, Rubelliform rashes, acute hemorrhagic conjunctivitis, aseptic meningitis, myocarditis, and neonatal infections.
HEV TaqMan RT-PCR Kit, 100 reactions
Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
Specific Primer and Probe mix for the pathogen/virus/viroid of interest
Primer and Probe mix
Positive and negative control to confirm the integrity of the kit reagents
HEV TaqMan RT-PCR Probe/Primer Set and Controls, 100 reactions
Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest
Nuclease-free water
Can be used together with Norgen’s RT-PCR Master Mix (#28113) or customer supplied master mix
For research use only and NOT intended for in vitro diagnostics.
Storage Conditions and Product Stability All kit components can be stored for 1 year after the date of production without showing any reduction in performance.
All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.
Attogene’s Microcystin Test Kit (Rapid – Recreational Water) can be used to detect microcystins in liquid samples; highly sensitive, rapid, robust screening kit for algae toxins microcystins and nodularins.
The most frequently reported cyanobacterial toxins are the hepatotoxic microcystins (MCs). MCs are peptides with a molecular weight ranging from 900 to 1,100 Da. They consist of seven amino acids of which the two terminal amino acids of the linear peptide are condensed to form a cyclic compound.
A tiered notification system which takes different actions based on different numeric thresholds for microcytin-LR concentrations in recreational waters has been developed. This is guidance that allows states to take various actions—such as posting information about harmful algal blooms (HABs), issuing a recreational public health advisory, or temporarily closing recreational waters through a no contact advisory—depending on the severity of the bloom event.
Microcystin-LR
Informational sign postings about HABs at recreational waters: < 6 μg/L Recreational public health advisory: 6 μg/L Elevated recreational public health advisory (e.g. no contact): 20 μg/L
Screening of Microcystins in water samples at 6 ppb or lower Format: 10 tests (5 tests/5 control) Water Sample Bottles Negative Control 1mL Syringe Sample Dilution Buffer also sold separately Sample Filters
