T7 RNA Polymerase

Introduction

Usages:
For isolating , cultivating mold and yeast .

Principle:
Potato flour leaching contribute various mold growth; glucose to provide energy; agar as medium coagulant; chloramphenicol inhibit the growth of bacteria.

Formulation(per liter):
Infusion from potatoes 200g
Glucose 20g
Agar 15g
Final pH 5.6 ± 0.2

How to use:
1.Suspend 40g in 1L of distilled water , stirring heated to boiling to completely dissolve ,autoclave at 121 for 15 minutes.
2.Diluted and treated samples.

Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.

500g

Description

Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.

The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit. Details of the target and priming specificity are included in the individual handbooks above.

Packaged, optimised and ready to use. Expect Better Data.

Primer and probe mix (150 reactions)

Reverse Transcription, target specific primers (RNA genome viruses only)

Copy number standard curve (sufficient for multiple standard curves)

Internal extraction control – Read through VIC channel*

Endogenous control (150 tests)

RNAse/DNAse free water

*alternative fluorophores available on request

Overview

• Free of all DNases, RNases and nucleic acids
• Prepared without the use of DEPC
• Room temperature storage

Norgen’s Nuclease-Free Water is free of all DNases, RNases and nucleic acids and is suitable for all molecular biology applications requiring nuclease-free water including PCR, RT-PCR and real-time PCR.  Our water is prepared without the use of DEPC, and is ready to use without further treatment.

Available Sizes

• 4 x 1.25 mL
• 100 x 1.25 mL
• 100 mL
• 500 mL
• 1000 mL

Details

Applications
For use in any molecular biology application

Storage Conditions
Norgen’s Nuclease-Free Water should be stored at room temperature. Storage at +4°C or -20°C is optional.

Precautions and Disclaimers
This product is designed for research purposes only.  It is not intended for human or diagnostic use.