Taq 2x PCR Master Mix – ready to use mix simplifies your PCR setup. Only primers and template need to be added as the mix contains all copmponents for a successful and reliable PCR. This ensures reproducible results, significantly reduces set-up times and the risk of pipetting errors. The robustness of PCR performance allows the application of this mix in a wide range of PCR amplifications.
Detail
Taq 2x PCR Master Mix – ready to use mix simplifies your PCR setup. Only primers and template need to be added as the mix contains all copmponents for a successful and reliable PCR. This ensures reproducible results, significantly reduces set-up times and the risk of pipetting errors. The robustness of PCR performance allows the application of this mix in a wide range of PCR amplifications. For research use and further manufacturing.In case you are aiming to use our RUO products as components or for your development of e.g. an IVD medical device, please contact us.
Broad Amplification Range
Different-sized amplicons from 3 ng of a DNA plasmid were amplified. The use of Taq DNA polymerase resulted in clean and high yield of products, as analysed after PCR on a 0.8% agarose gel.
Faster Detection and Higher Sensitivity
A fragment (64 bp) of the human blood-coagulation factor IIa (F2) was amplified from 20 ng, 2 ng, 200 pg and 20 pg of a human genomic DNA extract. The same experiment was performed in parallel using the Taq DNA polymerase mix from another well-established and known supplier. PCR products were subsequently analysed on a 2.5% agarose gel.
Other Products
Propargyl-PEG10-alcohol
Product Info
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Product Info
Propargyl-PEG10-alcohol is a heterobifunctional PEG linker which enables Click Chemistry reactions with azide-bearing biomolecules; copper catalyst is needed. The hydrophilic PEG units increase aqueous solubility. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
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Propargyl-PEG10-alcohol is a heterobifunctional PEG linker which enables Click Chemistry reactions with azide-bearing biomolecules; copper catalyst is needed. The hydrophilic PEG units increase aqueous solubility. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Norgen’s Saliva/Swab RNA Purification Kits provide a rapid method for the purification of total RNA from non-preserved saliva and nasal/throat swabs, and from preserved saliva collected on Norgen’s Saliva RNA Collection and Preservation Devices (Cat. RU53800) or preserved swabs collected in Norgen’s Total Nucleic Acid Preservative Tubes (Cat. 69200). Purification is based on using Norgen’s proprietary resin separation matrix. RNA is preferentially purified from other cellular components such as proteins, without the use of phenol or chloroform. The kit allows the purification of total RNA, including viral and bacterial RNA, irrespective of size or GC content. The purified RNA is eluted in an Elution Solution that is compatible with all downstream applications including PCR, qPCR, methylation-sensitive PCR and Southern Blot analysis, microarrays, and NGS.
Saliva/Swab RNA Purification Kit (Spin Column)
This kit offers a range of elution volumes, with a minimum elution volume of 75 μL and a maximum elution volume of 100 μL. Complete 10 purifications in as little as 15 minutes.
High throughput 96-well plate format for rapid purifications with very consistent well-to-well RNA isolation. Purified RNA is suitable for a variety of downstream applications , including Small RNA Sequencing. Find out more information about Norgen’s NGS Services.
Storage Conditions and Product Stability All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
This product is suitable for extracting RNA from anticoagulant blood, lymphocytes, buffy coat, bone marrow, cultured cells and other samples.
Details
Specifications
Features
Specifications
Main Functions
Isolation total RNA from 1-1.5ml whole blood, 0.5-1ml bone marrow, buffy coat
Applications
RT-PCR, cDNA synthesis, second generation sequencing
Purification method
Polydisperse magnetic beads
Purification technology
Magnetic beads technology
Process method
Manual or automatic
Sample type
anticoagulant blood, lymphocytes, buffy coat, bone marrow, cultured cells
Sample amount
1-1.5ml whole blood, 0.5-1ml bone marrow
Yield
1-30μg
Principle
This product is suitable for extracting RNA from anticoagulant blood, lymphocytes, buffy coat, bone marrow, cultured cells and other samples. This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested by lysis buffer and protease, and RNA/DNA is released into the lysis buffer. Add binding solution and magnetic particles to adsorb RNA/DNA, while proteins are not adsorbed and removed. The particles adsorbed with DNA/RNA are washed with washing buffer to remove proteins and other impurities, then washed with ethanol to remove salt, and finally digested with DNase to remove DNA. RNA is recovered by adding binding solution, and finally the RNA is eluted with low salt buffer. The eluted RNA can be directly used for experiments such as RT-PCR,NGS and virus detection.
Advantages
High purity – OD 260 / 280 :1.9-2.0, OD 260 / 230 :1.5-2.0
Economy – less than 50% of the price of top brands
Automatic – extraction without manual participation, saving time and effort
High yield – nucleic acid recovery up to 95%
General – samples include whole blood, serum, plasma, lymphocyte suspension, bone marrow, cultured cells, etc.
Kit Contents
Contents
R661101
R661102
R661103
Purification Times
48 Preps
96 Preps
480 preps
10 x RBC Lysis Buffer
50 ml
2 x 50 ml
4 x 100 ml
Proteinase K
12 mg
24 mg
120 mg
Protease Dissolve Buffer
1.8 ml
1.8 ml
10 ml
DNase I
600 μl
2 x 600 μl
10 x 600 µl
DNase Buffer
20 ml
30 ml
150 ml
MagPure Particles N
1.2 ml
2.5 ml
11 ml
Buffer RTL
30 ml
60 ml
300 ml
Buffer ALB2
40 ml
60 ml
300 ml
Buffer MW1*
22 ml
44 ml
220 ml
Buffer MW2*
20 ml
50 ml
2 x 100 ml
RNase Free Water
10 ml
20 ml
60 ml
Storage and Stability
DNase I should be shipped with ice pack or dry ice and stored at -20°C upon arrival. MagPure Particles N and Proteinase K should be stored at 2–8°C upon arrival. However, short-term storage(up to 8 weeks) at room temperature (15–25°C) does not affect their performance.The remaining kit components can be store at room temperature and are stable for up to 18 months under these conditions.
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This product is suitable for extracting RNA from anticoagulant blood, lymphocytes, buffy coat, bone marrow, cultured cells and other samples.
