Taq DNA Polymerase Hot-Start

Overview

• DNA can be isolated and detected from as little as 100 µL of sputum
• Effective removal of PCR inhibitors
• Compatible with Norgen’s Saliva Collection and Preservation Device (Cat. RU49000)
• Sputum Liquification Buffer (Cat. 28289) can be purchased separately

This kit constitutes an all-in-one system for the rapid isolation of DNA from sputum samples. It allows for the isolation of bacterial or eukaryotic DNA from the sputum samples using spin-column chromatography based on Norgen’s proprietary resin. The kit includes all the reagents needed to process sputum DNA. The protocol can be completed in 30 minutes. Purified DNA is of the highest quality and free from inhibitors, and can be used in sensitive downstream applications including PCR and qPCR.

Background

A sputum specimen is the name given to the mucus which is expectorated from the lower airways. High quality sputum samples should contain very little saliva, as this may contaminate the sputum sample with oral bacteria. Sputum samples are typically evaluated to look for infections such as Moraxella catarrhalis, Mycobacterium tuberculosis, Streptococcus pneumoniae ;and Haemophilus influenza. Other pathogens can be detected in sputum as well, including HIV. In addition, sputum samples are useful in the detection of lung cancer or to evaluate chronic inflammation.

Details

Supporting Data

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Kit Specifications
Time to Complete DNA Isolation	< 1 hour
Average Yield of DNA from 1.0 mL*	75 μg
Average Purity (OD260/280)	1.8 – 2.1

* Average DNA yield will vary depending on the health status of the donor

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.

Norgen’s Sputum DNA Isolation Kit contains ready-to-use Proteinase K, which is dissolved in a specially prepared storage buffer. The buffered Proteinase K is stable for up to 2 years after the date of shipment when stored at room temperature.

Component	Cat. 46200 (25 preps)
Slurry D	55 mL
Proteinase K in Storage Buffer	0.6 mL
Solution WN	4 mL
Wash Solution BE	9 mL
Elution Buffer B	8 mL
Mini Filter Spin Columns	25
Collection Tubes	25
Elution Tubes (1.7 mL)	25
Product Insert	1

K-PullG6

SKU: 700004329

100/200 assays per kit

Content:	100 / 200 assays per kit
Shipping Temperature:	Ambient
Storage Temperature:	Short term stability: 2-8oC, Long term stability: See individual component labels
Stability:	> 2 years under recommended storage conditions

Analyte:	Pullulanase/Limit-Dextrinase
Assay Format:	Spectrophotometer
Detection Method:	Absorbance
Wavelength (nm):	400
Signal Response:	Increase
Limit of Detection:	0.18 U/mL for pullulanase preparations (50-fold dilution) 0.01 U/g for limit dextrinase in milled malt
Reproducibility (%):	~ 3%
Total Assay Time:	~ 10 min (Pullanase), ~ 30 min (Limit-Dextrinase)
Application examples:	Assay of microbial pullulanase preparations. Measurement of limit-dextrinase in malt extracts.
Method recognition:	Novel method

PullG6 assay for the measurement of pullulanase employs a water soluble defined substrate, namely 4,6-O-benzylidene-4-nitrophenyl-6³-α-D-maltotriosyl-maltotriose (BPNPG3G3), coupled with the ancillary enzymes α-glucosidase and β-glucosidase. Upon hydrolysis of the substrate at the 1,6-α-linkage by pullulanase or limit-dextrinase, the released 4-nitrophenyl-β-maltotrioside is immediately hydrolysed to glucose and 4-nitrophenol by the concerted action of the α-glucosidase and β-glucosidase enzymes in the reagent mixture. The reaction is terminated and phenolate ions are developed by addition of dilute alkali. The absorbance is read at 400 nm and the value obtained correlates directly with pullulanase activity.

Explore more of our assay kit products for enzyme activity measurement.

Advantages

• High sensitivity 
• Suitable for manual and auto-analyser formats 
• No transglycosylation interference 
• Very cost effective 
• All reagents stable for > 1 year after preparation 
• Very specific
• Simple format
• Standard included

TD6 Table top laboratory centrifuge

TD6 Features:

1. Widely used in hospital, laboratory and blood bank.

2. Brushless DC motor for model TD6 which in great torque, free maintenance, no powder pollution, quick in speed up and down.

3. The flexible axle driven system which drive the rotor directly, smooth in operation, low noise and small vibration.

4. Micro computer control system, digital display the RCF、time and speed. There are 10 kinds of program and 10 kinds of acceleration and deceleration for your choice.

5. Electric lid lock, compact design, super speed and imbalance protection.

6. The centrifuge body is made of high-quality steel, safe and reliable.

TD6 Technical Parameters:

Max speed	6000rpm	Max RCF：	4430xg
Max volume	6x100ml	Noise：	≤55dBA
Timer	0～99min	Net weight：	32KG
Dimension(HxDxW)：	540×370×280mm	Power supply	AC 110/220V 50HZ/60hz
Speed accuracy	±20rpm	Acc/Dec	10 Kinds
Certifications	CE, ISO & Calibration report is available.	Warranty	1 Year

Matched Rotors for TD6:

Order No.	Rotor	Max Speed   (rpm)	Max Volume(ml)	Max. RCF(×g）
D6-1	Angle Rotor	6000	6×15/10/7/5ml	3660
D6-2	Angle Rotor	6000	12×15/10/7/5ml	4430
D6-3	Angle Rotor	6000	6×50ml	4110
D6-4	Angle Rotor	6000	4×50ml	3620
D6-5	Angle Rotor	6000	4×100ml	3790
D6-6	Angle Rotor	5000	24×15/10/7/5ml	3500
D6-7	Angle Rotor	4000	12×50ml	2470
D6-8	Angle Rotor	5000	6×100ml	3130
D6-9	Swing Rotor	5000	4x100ml	4420
D6-10	Swing Rotor	5000	4x50ml	4420
D6-11	Swing Rotor	5000	4x4x15ml	2820
D6-12	Swing Rotor	4000	4x6x10ml	2580
D6-13	Swing Rotor	4000	4x4x10ml	2580
D6-14	Microplate	4000	2x2x96well	2020