Norgen’s TaqMan 2x PCR Master Mix is a ready-to-use TaqMan 2x PCR Master Mix solution that contains a PCR internal control which can be detected by HEX/VIC channel in a real-time PCR machine. By detecting the internal control users can validate the DNA template quality, thereby preventing any false negatives in the PCR results. The user needs only to add template, target TaqMan primer/probe mix and water to set up the TaqMan real-time PCR.
PCR Control: TaqMan 2x PCR Master Mix contains PCR control primers/probe (HEX/VIC) and PCR control template. The PCR control reaction in the TaqMan 2x PCR Master Mix is optimized to not interfere with target amplification. The fluorescence of the target probe should not be HEX/VIC.
Details
Reagents Supplied – Cat # 28341
TaqMan 2x RT-PCR Master Mix (3 Vials, 100 Reactions) – Sufficient reagent for 100 x 20 µL reactions
Storage Conditions and Product Stability Norgen’s TaqMan 2x PCR and 2x RT-PCR Master Mixes should be stored at -20ºC. For everyday use an aliquot can be stored at 4ºC for up to three months. The Master Mix is stable for multiple freeze-thaw cycles. When stored at the proper temperature this reagent is stable for at least 1 year.
Other Products
12917 MagPure Circulating DNA Rich Mini Kit
Product Info
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Product Info
Introduction
MagPure Circulating DNA Rich Kit designed for purification of high quality circulating DNA (cfDNA) from cell-free body fluids (such as plasma, serum). The purified DNA is suitable for direct use in downstream applications such as PCR, real-time PCR, biochip analysis and NGS.
Details
Specifications
Features
Specifications
Main Functions
Rich small fragment cfDNA from 0.6ml serum plasma, remove DNA fragments＞500bp
Applications
qPCR, NGS, etc.
Purification technology
Magnetic beads technology
Process method
Manual or automatic
Sample type
Serum, plasma
Sample amount
0.6ml
Elution volume
Time per run
Principle
This product is based on the purification method of high binding magnetic particles. The sample is lysedand digested under the action of lysate and Protease. DNA is released into the lysate. After adding magnetic particles and binding solution, Large DNA(>500bp) will be adsorbed on the surface of magBindparticles. After removal of large size, small size of DNA(<500bp) will be adsorbed on the surface ofMagPure Particle F and impurities such as proteins will be removed without adsorption. The adsorbedparticles were washed with washing solution to remove proteins and impurities, washed with ethanol toremove salts, and finally DNA
Advantages
Economy – less than 50% of the price of Qiagen and other imported products
Automatic – without labour
Kit Contents
Contents
1292750
12927200
Purification Times
50 preps
200 preps
MagPure Particles F
1.2 ml
4.5 ml
MagBind Particles
1.2 ml
4.5 ml
Proteinase K
24 mg
90 mg
Protease Dissolve Buffer
1.8 ml
10 ml
Buffer MLB
30 ml
120 ml
Buffer GDP
15 ml
60 ml
Buffer MAW1
40 ml
250 ml
Buffer MW2*
20 ml
50 ml
Elution Buffer
15 ml
60 ml
Storage and Stability
Proteinase K, MagBind Particles and MagPure Particles F should be stored at 2–8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2-8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.
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MagPure Circulating DNA Rich Kit designed for purification of high quality circulating DNA (cfDNA) from cell-free body fluids (such as plasma, serum). The purified DNA is suitable for direct use in downstream applications such as PCR, real-time PCR, biochip analysis and NGS.
CE-IVDR marked in accordance with the European Commission Regulation (EU) No. 2017/746.
Available for sale in Canada (Dx17200, Dx24300)
Ideal for use in in vitro diagnostic workflows
Extract high quality & quantity total RNA including miRNA
No phenol step required
Bind & elute all RNA irrespective of size or GC content, without bias
Very sensitive & linear down to a few cells without the need for carrier RNA
Convenient & fast spin column format
Available in 96-Well and 96-Well (Deep Well) formats for high throughput automation
Isolate from a wide variety of specimens
Purified RNA is suitable for a variety of downstream applications
Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix
Norgen’s Total RNA Purification Kits Dx provide rapid methods for the isolation and purification of total RNA from tissue samples, blood, plasma, serum, saliva, bacteria, yeast, fungi and viruses for subsequent in vitro diagnostic use. Extract high quality and purity RNA with excellent RIN values and A260/A280 suitable for downstream applications including qRT-PCR, RT-PCR, microarrays, NGS and more.
Total RNA Purification Kit Dx (Spin Column)
This kit is designed to be used with any downstream application employing enzymatic amplification or other enzymatic modifications of RNA followed by signal detection or amplification. Any diagnostic results generated using the RNA isolated with Norgen’s Total RNA Purification Kit Dx in conjunction with an in vitro diagnostic assay should be interpreted with regard to other clinical or laboratory findings.
Total RNA Purification 96-Well Kits Dx (High Throughput and High Throughput Deep Well)
Thess 96-well kits provide a rapid method for the high-throughput isolation and purification of total RNA in 30 minutes using vacuum manifold, plate centrifuge, or liquid handlers with vacuum capabilities. Total RNA can be isolated from a broad range of sample sources including cultured cells, tissues, blood, serum, plasma, bacteria, yeast, fungi, and viruses.
Intended Use
To minimize irregularities in diagnostic results, suitable controls for downstream applications should be used. Norgen’s Total RNA Purification Kits Dx are intended for use by professional users such as technicians, physicians and biologists experienced and trained in molecular biological techniques. Norgen’s Total RNA Purification Kits Dx do not provide a diagnostic result. It is the sole responsibility of the user to use and validate the kit in conjunction with a downstream in vitro diagnostic assay.
NOTE: This product is not available for sale in the United States.
Average Yield HeLa Cells (1 x 106 Cells) E. coli (1 x 109 cells) Brain (10 mg) Liver (10 mg) Blood (50 μL, Hamster)
15 μg 50 μg 9 μg 26 μg 1 μg
Maximum Amount of Starting Material: Whole Blood Plasma/Serum Bacteria Yeast Fungi Plant Tissue
100 µL150 µL1 x 109 cells1 x 108 cells 40 mg 40 mg
Storage Conditions and Product Stability All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers.
Propargyl-PEG9-alcohol is a crosslinker that can participate in copper catalyzed azide-alkyne Click Chemistry reactions to form stable triazole linkage. The PEG spacer increases the hydrophilicity of the molecule in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Document
Propargyl-PEG9-alcohol is a crosslinker that can participate in copper catalyzed azide-alkyne Click Chemistry reactions to form stable triazole linkage. The PEG spacer increases the hydrophilicity of the molecule in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
