
TCO-PEG2-DBCO is a short PEG linker featuring a trans-cyclooctene and a DBCO group. DBCO is a click chemistry handle which easily reacts with azides, while the TCO function readily reacts with tetrazine-containing compounds.

TCO-PEG2-DBCO is a short PEG linker featuring a trans-cyclooctene and a DBCO group. DBCO is a click chemistry handle which easily reacts with azides, while the TCO function readily reacts with tetrazine-containing compounds.
TCO-PEG2-DBCO is a short PEG linker featuring a trans-cyclooctene and a DBCO group. DBCO is a click chemistry handle which easily reacts with azides, while the TCO function readily reacts with tetrazine-containing compounds.
The HiPure Mini system provides a fast, simple, and cost-effective plasmid DNA miniprep method for routine molecular biology laboratory applications. HiPure Plasmid Mini Kits use silica membrane technology to eliminate the cumbersome steps associated with loose resins or slurries. Plasmid DNA purified with Mini Kits is immediately ready for use. Phenol extraction and ethanol precipitation are not required, and high quality plasmid DNA is eluted in a small volume of Tris buffer or water.
Specifications
Features | Specifications |
Main Functions | Isolation up to 70μg plasmid DNA from 5-15ml bacterial culture |
Applications | Enzyme digestion, sequencing, PCR, cloning, etc. |
Purification method | Mini spin column |
Purification technology | Silica technology |
Process method | Manual (centrifugation or vacuum) |
Sample type | Conventional plasmid, plasmid less than 30KB |
Sample amount | High copy plasmid: 1-5ml culture mediumLow copy plasmid : 5-10ml culture medium |
Yield | 20-80µg |
Elution volume | ≥75μl |
Time per run | Complete 1-24 samples in 30 minutes |
Liquid carrying volume per column | 800µl |
Binding yield of column | 70µg |
The HiPure Plasmid procedure is based on alkaline lysis of bacterial cells followed by adsorption of DNA onto silica in the presence of high salt. The unique silica membrane used in the kit completely replaces glass or silica slurries for plasmid DNA minipreps. The procedure consists of 3 basic steps: Preparation and clearing of a bacterial lysate by alkaline method,then transfer the supernatant to column to bind DNA. After washing proteins and other impurities, nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).
Advantages
Kit Contents
Contents | P100202 | P100203 |
Purification Times | 50 Preps | 250 Preps |
RNase A | 5 mg | 20 mg |
Buffer P1 | 30 ml | 140 ml |
Buffer P2 | 30 ml | 140 ml |
Buffer P3 | 40 ml | 200 ml |
Buffer PW1 | 30 ml | 140 ml |
Buffer PW2* | 12 ml | 50 ml |
Elution Buffer | 15 ml | 30 ml |
HiPure DNA Mini Columns III | 50 | 250 ml |
2 ml Collection Tubes | 50 | 250 ml |
Storage and Stability
The kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. If any precipitates form in the buffers, warm at 37℃ to dissolve. After addition of RNase A,Buffer P1 is stable for 6 months when stored at 2-8°C.
For any technical problems or customized products, please contact us.
F&Q about Endotoxin-free Plasmid Extraction Kit — P1156 ←click here
The HiPure Mini system provides a fast, simple, and cost-effective plasmid DNA miniprep method for routine molecular biology laboratory applications. HiPure Plasmid Mini Kits use silica membrane technology to eliminate the cumbersome steps associated with loose resins or slurries. Plasmid DNA purified with Mini Kits is immediately ready for use. Phenol extraction and ethanol precipitation are not required, and high quality plasmid DNA is eluted in a small volume of Tris buffer or water.
Product Description
Optimized Nucleic Acid Reagent for DNA Isolation
Product Description:
Amp-future Bio’s Nucleic Acid Extraction Reagent is a high quality and reliable DNA extraction kit that is specifically designed for automated nucleic acid extraction. It has a shelf life of 12 months and comes in a convenient liquid form. This product is the perfect choice for researchers and laboratories that require fast and accurate results, as it offers a highly efficient solution for extracting nucleic acids from a variety of biological samples. It is easy to use and can be used in either manual or automated protocols. The reagent contains a broad spectrum of components that are essential for the successful isolation and extraction of nucleic acids. With its automated nucleic acid extractor, it ensures that all samples are processed with maximum efficiency and accuracy.
Features:
Technical Parameters:
Product Name | Technical Parameters |
---|---|
Nucleic Acid Extraction Reagent | Application: DNA Extract Form: Liquid Shelf Life: 12 Months Brand: Amp-future Bio Automated Nucleic Acid Extractor, DNA Extraction Kit, Automated Nucleic Acid Extractor |
Applications:
Amp-future Bio Nucleic Acid Extraction Reagent is a liquid reagent specially formulated for automated nucleic acid extraction. It is widely used in DNA/RNA extraction from various sample matrixes, such as blood, tissue, cells, bacteria, etc. It ensures high-quality nucleic acid extraction in a short period of time. The reagent is produced in China and has a shelf life of 12 months.
Amp-future Bio Nucleic Acid Extraction Reagent is suitable for use with the Automated Nucleic Acid Extractor. It is highly efficient and reliable, allowing for fast and accurate nucleic acid extraction. The reagent is also suitable for use with the DNA Extraction Kit, which makes it ideal for clinical laboratories. It provides superior nucleic acid extraction performance.
Amp-future Bio Nucleic Acid Extraction Reagent is an essential product for laboratories that need to extract nucleic acids from various sample matrixes. It is highly reliable and efficient, providing superior results in a short period of time. The reagent is also suitable for use with the Automated Nucleic Acid Extractor and the DNA Extraction Kit. It is a reliable and cost-effective product for laboratories that require high quality nucleic acid extraction.
Customization:
Customized Nucleic Acid Reagent Service
Amp-future bio provides custom nucleic acid reagent services to meet your specific needs. We specialize in the production of DNA/RNA extraction reagents for automated nucleic acid extraction, purification and other related applications.
Our products are suitable for DNA extraction, automated nucleic acid extraction, nucleic acid purification, and other related applications. We are confident that our products can meet the highest quality standards and provide the best performance. We are dedicated to providing the best customer service and technical support for our customers.
Support and Services:
Our team of experts provide the highest quality technical support and services for Nucleic Acid Reagents products.
Our technical support staff are available to assist you with any questions or troubleshooting related to the use of Nucleic Acid Reagents. Our team can provide guidance on the proper use and handling of our products, as well as any other technical questions. We offer technical support via phone, email, and online chat.
We also offer a variety of services to support our customers in the use of Nucleic Acid Reagents. These services include:
Amp-future Bio’s Nucleic Acid Extraction Reagent is a high quality and reliable DNA extraction kit that is specifically designed for automated nucleic acid extraction. It has a shelf life of 12 months and comes in a convenient liquid form. This product is the perfect choice for researchers and laboratories that require fast and accurate results, as it offers a highly efficient solution for extracting nucleic acids from a variety of biological samples. It is easy to use and can be used in either manual or automated protocols. The reagent contains a broad spectrum of components that are essential for the successful isolation and extraction of nucleic acids. With its automated nucleic acid extractor, it ensures that all samples are processed with maximum efficiency and accuracy.
Safety Features
1. User-Friendly Design: The chamber lid and tabletop are covered with heat-insulating resin materials, providing both protection against burns and an aesthetically pleasing appearance. The upward-opening flip-top door saves space, while the rounded corners prevent injury. The specially designed steam discharge and collection system minimizes steam burn risks and enhances operational convenience.
2. Interlock Mechanism and Safety Protection: The safety interlock system is designed to be more sensitive and secure. The chamber lid cannot be opened if there is pressure or steam inside the chamber. The lid can only be opened when the pressure gauge reads “0” MPa. The sterilizer will not start heating if the chamber lid is not properly locked. These features enhance the overall safety of the machine.
3. Over-temperature and Heating Protection: The system continuously monitors the temperature inside the sterilization chamber. If the temperature becomes too high or heating is abnormal, power will be automatically cut off to prevent overheating.
4. Automatic Fault Detection System: The machine performs an automatic self-check when powered on. If a fault occurs during operation, an audible alarm will sound, and a fault code will be displayed.
5. Lid Closure Check System: The system automatically detects whether the chamber lid is securely locked. If the lid is not properly locked, the sterilizer will not start.
6. Leakage, Over-current, and Short-Circuit Protection: If there is leakage in the heating elements or other components, or if over-current or short-circuit conditions occur, the system will automatically cut off power to prevent user injury.
7. Water Shortage and Independent Dry-Burning Protection: Equipped with a low water level sensor and independent dry-burning protection device, the system can monitor the water level in the chamber in real-time to prevent dry burning and avoid false readings. If the low water level sensor fails, the overheat and dry-burning protection system will automatically activate, cut off power to the heating ring, and sound an alarm to ensure safety.
8. Dual Pressure Protection: The system includes both a mechanical safety valve and an electronic over-pressure protection system. If pressure becomes abnormal, the system will relieve pressure and cut off power while sounding an alarm.
9. Back end Safety Testing: The system can be periodically tested for safety and protective measures.
10. Over-pressure Testing: Regular testing ensures that the instrument’s over-pressure protection system can automatically cut off power or that the safety valve can open to relieve pressure.
11. Performance Testing: The instrument’s safety performance has been tested by national authorized inspection agencies, and a test report is available.
Technical Parameters
Model | HB65A Standard TypeHB65B Auto Water Replenishment TypeHB65C Auto Drying Type | HB90AStandard TypeHB90BAuto Water Replenishment TypeHB90CAuto Drying Type | HB110AStandard TypeHB110BAuto Water Replenishment TypeHB110CAuto Drying Type | |
Capacity | 65L | 90L | 110L | |
Dimensions (L × W × H) mm | 664×660×994.5 | 664×660×994.5 | 664×660×1200 | |
Sterilization Chamber Dimensions (Diameter × Height) mm | φ400×530 | φ400×730 | φ400×920 | |
Rated Power | 2800W | 3400W | 4500W | |
Rated Voltage | 220V±10%50Hz | |||
Temperature Display Accuracy | 0.1℃ | |||
Operating Environment | 5℃~40℃、Relative Humidity10%~85% | |||
Sterilization Chamber Material | SUS304Stainless Steel | |||
Sterilization Working Temperature | 105℃~143℃ | |||
Sterilization Time Pre-set Range | 1minute ~9999minutes | |||
Melting Temperature Pre-set Range | 60℃~100℃ | |||
Melting Time Pre-set Range | 1minute~9999minutes | |||
Insulation Temperature Pre-set Range | 45℃~60℃ | |||
Insulation Time Pre-set Range | 1minute~9999minutes | |||
Door Opening Temperature Pre-set Range | Solid, Agar, and Waste Modes: 40°C ~ 99°C Liquid Mode: 40°C ~ 80°C | |||
Clock FunctionTimer Pre-set Range | Timer Pre-set Range: Set operating time (year, month, day, hour, minute), check current time (year, month, day, hour, minute), total sterilization cycles, fault occurrence time, and code. | |||
Pressure Gauge Display Range | 0~0.6Mpa | |||
Pressure | Chamber Design Pressure: 0.35 MPa; Each product comes with a quality certificate and a certificate issued by the special equipment manufacturing supervision and inspection department. | |||
Pressure Safety Protection | Dual independent pipeline measurement and control, mechanical and electronic dual pressure protection | |||
Sterilization Modes | · Solid Mode: Heating → Sterilization → Exhaust, Air Cooling· Solid with Drying Mode: Heating → Sterilization → Drainage, Exhaust → Drying· Liquid Mode: Heating → Sterilization → Pre-cooling → Exhaust· Liquid with Insulation Mode: Heating → Sterilization → Pre-cooling → Exhaust → Insulation· Waste Mode: Heating → Sterilization → Exhaust, Air Cooling· Agar Mode: Heating → Melting → Insulation | |||
Exhaust Control and Water Tank | Fully automatic internal exhaust (see instructions for exhaust temperature settings), built-in large-capacity steam condensate collection bottle and water tank | |||
Cover Opening Method and Interlocking Device | Unique eight-column interlocking device, flip-cover switch, touch-type handle | |||
Controller and Memory Storage Function | Microcomputer intelligent control system. 6 fixed operation modes, 82 modifiable modes, 88 memory storage programs | |||
Safety Devices | Door lock interlocking device; Cover closing check; Dry burn protection; Water level sensor monitoring; Electronic over pressure protection; Mechanical safety valve; Over temperature and heating protection; Over current and short circuit protection; Leakage protection; Cooling lock; Burn injury safety protection; Automatic fault detection system | |||
Accessories | Condensate collection bottle, water separation plate, drainage hose, stainless steel basket | |||
Optional Accessories | Printer, cooling fan, item temperature sensor, USB output | |||
Stainless Steel Baskets | (φ360×230)1pcs(φ360×200)1pcs | (φ360×200)1pcs(φ360×400)1pcs | (φ360×200)2pcs(φ360×400)1pcs |
Note: The upper limits for cooling lock door temperature and exhaust temperature are related to the boiling point.
Other Optional Accessories
1. Dot Matrix Printer: Capable of printing temperature, time, F0 value, and pressure data. USB data export and curve plotting.
2. Stainless Steel Basket with Base.
3. Pressure Sensor
4. Stainless Steel Bucket
5. Item Thermometer
6. 3Q Validation Adapter
Features and functions:
1. Large-diameter sterilization chamber:The 40 cm large-diameter sterilization chamber provides an exceptionally spacious environment to meet a wide range of sterilization needs.
2. Sterilization temperature:105-138℃.
3. Exhaust control :After sterilization is complete, the exhaust valve can automatically open to release the exhaust at a pre-set temperature.
4. Verification interface: Provides temperature and pressure inspection ports for easy calibration.
5. Saturated steam monitoring:The system automatically monitors the expulsion of cold air to ensure a pure steam sterilization environment, guaranteeing optimal sterilization effectiveness.
6. Pressure performance:The chamber is designed to withstand a pressure of up to 0.35 MPa, and each unit is tested with a water pressure of up to 0.44 MPa. Advanced pressure protection ensures the safety load of the equipment.
7. Intelligent control system:The microcomputer chip-based intelligent control system monitors the temperature and pressure inside the sterilization chamber in real-time. The chamber lid can only be opened under safe temperature and pressure conditions. The system also provides fully automatic control of the sterilization process and allows for convenient maintenance, inspection, calibration, recording, and future upgrades.
8. Six preset programs:Includes six fixed programs for liquid sterilization and holding, solid sterilization and drying, agar melting and holding, and waste sterilization.
9. Memory storage system:Users can set their own parameters, create multiple programs, and access them at any time. The system allows for operation permissions to be set at three levels—user, administrator, and engineer—to facilitate effective use and management of the sterilizer.
10. Door-opening temperature: Each program can be set with a specific cooling lock door-opening temperature based on the thermal inertia of the sterilized items. This prevents users from opening the door when the items (especially liquid sterilized items) are still in an unsafe condition, thus avoiding burns.
11. Drying function: Thorough drying that meets CE standards, with a cooling lock mechanism.
12. One-touch inquiry function: Allows checking of current time, total number of sterilization cycles, fault occurrence time, and codes.
13. Scheduled start function: Allows you to set a start time so that the sterilizer automatically starts at the scheduled time.
Model:
HB65/ HB90/ HB110
83, On-nut 88/2 Prawet Sub-district, Prawet District, Bangkok, 10250, Thailand
Tel : 081-875-1869 , 02-328-7179
Email : hej@a3p-scientific.com
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