Introduction

This product is suitable for rapid extraction of total DNA from tissue, cells, blood, saliva, swabs, blood spots, semen and other clinical samples. DNA can be used directly for PCR, quantitative PCR, Southern Blot, test of virus DNA and so on.

Details

Specifications

Features	Specifications
Main Functions	Isolation total DNA from tissue / blood / body fluid / swab /dry blood spots
Applications	PCR, qPCR, southern bolt and virus detection, etc.
Purification method	Mini spin column
Purification technology	Silica technology
Process method	Manual (centrifugation or vacuum)
Sample type	Tissue, cell, blood, saliva, swab, blood spot, semen and other clinical samples
Sample amount	Solid tissue : 1-10mg, Anticoagulant blood : 200µl
Yield	1 – 15µg
Elution volume	≥20μl
Time per run	30 – 60 minutes
Liquid carrying volume per column	750µl
Binding yield of column	100µg

Principle

This product is based on silica Column purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption column. Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).

Advantages

• High quality DNA – meet a variety of downstream applications, including PCR, qPCR, enzyme digestion, hybridization, etc.
• Fast – without separation of leukocytes, organic extraction or ethanol precipitation
• Simple – all nucleic acids can be obtained by direct digestion
• Wide applicability – It can handle various liquid samples, animal tissues and cultured cells

Kit Contents

Contents	IVD3018
Purification Times	100
HiPure DNA Mini Columns I	100
2ml Collection Tubes	2 x 100
Buffer ATL	60 ml
Buffer AL	60 ml
Buffer GW1	44 ml
Buffer GW2	50 ml
Proteinase K	60 mg
Protease Dissolve Buffer	5 ml
Buffer AE	15 ml

Storage and Stability

Proteinase K should be stored at 2–8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2–8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.

Experiment Data

This product is suitable for rapid extraction of total DNA from tissue, cells, blood, saliva, swabs, blood spots, semen and other clinical samples. DNA can be used directly for PCR, quantitative PCR, Southern Blot, test of virus DNA and so on.

Description

Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.

The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit. Details of the target and priming specificity are included in the individual handbooks above.

Packaged, optimised and ready to use. Expect Better Data.

Primer and probe mix (150 reactions)
Reverse Transcription, target specific primers (RNA genome viruses only)
Copy number standard curve (sufficient for multiple standard curves)
Internal extraction control – Read through VIC channel*
Endogenous control (150 tests)
RNAse/DNAse free water
*alternative fluorophores available on request

Features:

Large capacity water capture freeze dryer

Tabletop medium-sized freeze dryer

Suitable for drying medium-sized samples

Apply to drying in laboratories such as cosmetics, fermentation broth, soil, materials, etc.

product description:

9kg-12kgs water capture capacity, which can meet the needs of laboratories with large freeze-drying requirements for water capture capacity

-70°C/-90°C ultra-low temperature cold trap, which can capture lower temperature organic solvents

Unique refrigeration technology, the cooling time of the cold trap from the start of the low-temperature refrigeration system to the target value is ≤3 minutes

Direct-capture large coil cold trap, higher efficiency, better ice capture effect

Hemispherical cold trap bottom surface is more conducive to the discharge of condensed water

The cold trap and condenser coil are all Teflon anti-corrosion treatment, which can freeze-dry a large amount of ultra-low temperature organic solvents

One-click hot gas defrost, with defrost compressor protection program.

Large flat flange silicone seal, permanently no need to apply any auxiliary sealing silicone grease

High-precision ultra-wide width detection, vacuum sensor with full-range automatic temperature compensation, making vacuum display more accurate

The cavity vacuum can be read and controlled accurately, and the vacuum control accuracy is ≤0.1mbar

Removable sample shelf with dedicated holes for centrifuge tube placement, making placement and height adjustment easier

Configuration of various types of chambers and T-frames can fully satisfy users’ sample placement

Optional configuration of eutectic point detection system and data export system.

The vacuum pump and sample placement chamber can be combined at will according to user needs, making the configuration more flexible and convenient

Techinical parameter

9-12L Floor tabletop and floor standing one for lab usage

Tabletop	BD1-9	BD2-9	BD1-12	BD2-12
Floor standing	BH1-9	BH2-9	BH1-12	BH2-12
Water Capture capacity	9L		12L
Cold trap temperature	-70 C	-90℃	-70C°	-90℃
Cold trap volume	15L		20L
Freeze dried chamber	High permeability organic glass/multi manifold external valve/stainless steel chamber
Sample shelf	Stainless steel φ 260mm/485mm. 3-10 layers/optional●Electric heating/manual capping/optional
Vacuum pump	Extreme vacuum 4 * 10-4mbar, pumping capacity ≥ 200L/can be equipped with various types of pumps
Oil mist filter	Standard configuration
Vacuum pump connection pipe	Single forming stainless steel flexible pipe DN16  ISO-KF L-1.5m
anti-corrosive treatment	Cold trap, condensing coil/all equipped with PTFE anti-corrosion treatment as standard. Freeze-drying organic solvents
External valve	1-48 ports/optional
Eutectic point judgment	Support/Optional
Data audit trail	Support/Optional
Power	0.9kw	1.5kw	1kw	1.6KW
Size	390*468*448mm	480*498*487mm