TDL5B refrigerated low speed centrifuge 

Overview

• Isolate total DNA and RNA from all microorganisms found in water, including bacteria, fungi and algae
• RNA and DNA are both column purified simultaneously using the same column
• Elution contains concentrated DNA and RNA without the need for further precipitation
• Complete RNA (including microRNA) without phenol
• Isolated RNA and DNA are of high quality and integrity for all downstream applications
• Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

These kits provide a convenient and rapid method for the isolation of microorganisms from water samples. The kits allows for the rapid isolation and purification of total RNA and DNA simultaneously from the microorganisms found in different types of water samples. The total RNA and DNA (including genomic DNA) are isolated from all the microorganisms found in the water, including bacteria, fungi and algae without the use of any inhibitory organic substances The kit purifies genomic DNA, and all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA). The purified RNA and DNA are highly concentrated, and can be used directly in a number of downstream applications including PCR, qPCR, RT-PCR, qRT-PCR, Northern blotting, Southern blotting and sequencing reactions.

Water RNA/DNA Purification Kit (Spin Column + Filters Kits)

These kits are available with 0.45 μm or 0.22 μm filter formats for capturing microorganisms present in the water.

Water RNA/DNA Purification Kit (Spin Column)

This kit is sold without filters columns.

Details

Supporting Data

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Kit Specifications
Minimum Water Input	10 mL
Maximum Water Input	100 mL
Maximum Filter Column Loading Volume	20 mL
Maximum Spin Column Loading Volume	650 µL
Elution Volume	100 µL
Time to Complete 10 Purifications	45 minutes

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years from the date of shipment.

Component	Cat. 26400 (25 preps)	Cat. 26450 (25 preps)	Cat. 26480 (50 preps)
Lysis Buffer E	15 mL	15 mL	2 x 15 mL
Wash Solution A	18 mL	18 mL	38 mL
Enzyme Incubation Buffer B	6 mL	6 mL	6 mL
Elution Buffer H	6 mL	6 mL	6 mL
Mini Spin Columns	25	25	50
Filter Columns (0.22 μL)	25	–	–
Filter Columns (0.45 μL)	–	25	–
Bead Tubes	25	25	50
Collection Tubes	25	25	50
Elution Tubes (1.7 mL)	25	25	50
Product Insert	1	1	1

Description

Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens. A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.

The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit.

Details of the target and priming specificity are included in the individual handbooks above.

Packaged, optimised and ready to use. Expect Better Data.

Exceptional value for money
Rapid detection of all clinically relevant subtypes
Positive copy number standard curve for quantification
Highly specific detection profile
High priming efficiency
Broad dynamic detection range (>6 logs)
Sensitive to < 100 copies of target Accurate controls to confirm findings 150 reactions

DBCO-PEG23-amine is a PEG linker which contains DBCO and amine moieties. The DBCO group is commonly used in copper-free Click Chemistry reactions. The amine group is reactive with carboxylic acids, activated NHS esters, carbonyls (ketone, aldehyde) etc. The hydrophilic PEG spacer increases the water solubility of the compound. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

DBCO-PEG23-amine is a PEG linker which contains DBCO and amine moieties. The DBCO group is commonly used in copper-free Click Chemistry reactions. The amine group is reactive with carboxylic acids, activated NHS esters, carbonyls (ketone, aldehyde) etc. The hydrophilic PEG spacer increases the water solubility of the compound. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.