TDL5E Bench top low speed refrigerated centrifuge machine

TDL5E is a table top low speed refrigerated centrifuge widely used in the field of biochemistry, biological products, pharmaceutical factory and laboratory.

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TDL5E Bench top low speed refrigerated centrifuge machine

Application:

Widely used in the field of biochemistry, biological products, pharmaceutical factory and laboratory.

TDL5E Technical Parameters:

Max. Speed	5000rpm	Max.RCF	4420xg
Max. Volume	4x180ml	Speed Accuracy	±20rpm
Timer	1~99h59min	Noise	≤60dBA
Dimension(LxWxHmm)	570x600x340mm	Net Weight	76Kg
Power Supply	AC220V/110V 50HZ/60HZ	Temperature Range	-20℃~40℃
Temperature Accuracy	±1℃	Certifications	CE, ISO & Calibration report is available.

Matched Rotors for TDL5E:

Order No.	Rotor Type	Max. Speed(rpm)	Volume(ml)	Max.RCF(xg)
5E-1	Swing rotor	4000	4x180ml	2580
5E-2	Microplate rotor	4000	2x3x96well	2020
5E-3	Swing rotor	5000	4x1x50ml	4420
5E-4	Swing rotor	5000	4x1x100ml	4420
5E-5	Swing rotor	4000	4x2x50ml	2820
5E-6	Swing rotor	4000	4x4x15ml	2820
5E-7	Swing rotor	4000	4x6x10/7ml	2580
5E-8	Swing rotor	4000	4x6x5ml	2170
5E-9	Swing rotor	4000	4x4x10/7ml	2580
5E-10	Swing rotor	4000	4x4x5ml	2170
5E-11	Fixed rotor	5000	6x15ml	2540
5E-12	Fixed rotor	5000	12x15ml	3080
5E-13	Fixed rotor	5000	24x15ml	3500
5E-14	Fixed rotor	5000	4x50ml	2520
5E-15	Fixed rotor	5000	6x50ml	2850
5E-16	Fixed rotor	5000	4x100ml	2630
5E-17	Fixed rotor	5000	6x100ml	3130

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4×7×50ml Swing Rotor

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Plasma/Serum Circulating DNA Purification Kits (Slurry Format)

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Overview

Isolate all sizes of circulating DNA from plasma and serum samples
Isolate Viral and Bacterial DNA
Versatile plasma and serum input volumes
Concentrate circulating DNA into small elution volumes
Different elution strategies depending on the downstream application
Isolate inhibitor-free circulating DNA for any application including PCR, qPCR, methylation-sensitive PCR
Compatible with Streck Cell-Free DNA BCT® Tubes
Isolate DNA with high quality and quantity from milk
Purification is based on Norgen’s proprietary Silicon Carbide resin matrix

Norgen’s Plasma/Serum Circulating DNA Purification Kits (Slurry Format) provide efficient methods for the purification of fragmented free-circulating DNA from human plasma or serum. The kit is able to isolate all sizes of circulating DNA. The slurry format provides an advantage over other available kits in that it does not require extension tubes for the purification of free-circulating DNA from large sample volumes. DNA can be isolated from either fresh or frozen samples using this kit. The kit will also isolate viral and bacterial DNA from plasma/serum. Typical yields of purified free-circulating DNA will vary depending on the input sample (1-100ng/mL circulating DNA in human plasma), with more concentrated samples tending to yield more free-circulating nucleic acids. The purified, inhibitor-free plasma/serum free-circulating DNA is eluted in an elution solution that is compatible with PCR, qPCR, methylation-sensitive PCR and Southern Blot analysis.

Plasma/Serum Circulating DNA Purification Mini Kit (Slurry Format)

Norgen’s Plasma/Serum Circulating DNA Purification Mini Kit (Slurry Format) provides a fast, reliable and simple procedure for isolating circulating DNA from various amounts of plasma/serum ranging from 50 μL to 400 μL. Preparation time for a single sample is less than 30 minutes.

Plasma/Serum Circulating DNA Purification Midi Kit (Slurry Format)

Norgen’s Plasma/Serum Circulating DNA Purification Midi Kit (Slurry Format) provides a fast, reliable and simple procedure for isolating circulating DNA from various amounts of plasma/serum ranging from 400 μL to 2 mL. Preparation time for a single sample is less than 45 minutes.

Plasma/Serum Circulating DNA Purification Maxi Kit (Slurry Format)

Norgen’s Plasma/Serum Circulating DNA Purification Maxi Kit (Slurry Format) provides a fast, reliable and simple procedure for isolating circulating DNA from various amounts of plasma/serum ranging from 2 mL to 10 mL. Preparation time for a single sample is less than 45 minutes.

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Supporting Data

Figure 1 / 10

Figure 1. Isolation of Circulating DNA from Different Plasma Volumes

Figure 1. Isolation of Circulating DNA from Different Plasma Volumes

Figure 2. Isolation of Viral DNA from Plasma

Figure 3. Reproducible Recovery of Circulating DNA

Figure 4. Reproducible Recovery of Circulating DNA

Figure 5. Detection of Human 5S Gene from Plasma

Figure 6. Determination of Rate of Inhibition in Plasma DNA Samples

Figure 7. Detection of Human 5S and S15 Genes

Figure 8. Detection of Spiked Viral DNA from 5 mL of Plasma

Figure 9. Scalability of the Plasma/Serum Circulating DNA Purification Maxi Kit (Slurry Format)

Figure 10. Determination of the Rate of Inhibition Present in Plasma DNA Samples

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Kit Specifications
Minimum Plasma/Serum Input	2 mL
Maximum Plasma/Serum Input	10 mL
Size of DNA Purified	≥ 50 bp
Elution Volume	100 μL
Time to Complete 10 Purifications	15-20 minutes
Average Yields	Variable depending on specimen

Note: Do not exceed the recommended sample input volume of 10 mL.

Storage Conditions and Product Stability

All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.

Component	Cat. 50600 (50 preps)	Cat. 51200 (20 preps)	Cat. 51300 (10 preps)
Lysis Buffer D	65 mL	125 mL	3 x 125 mL
Slurry A1	–	6 mL	–
Slurry A2	12 mL	–	12 mL
Binding Buffer B	20 mL	6 mL	20 mL
Binding Buffer C	–	–	30 mL
Wash Solution A	2 x 38 mL	2 x 38 mL	3 x 38 mL
Elution Buffer B	8 mL	8 mL	15 mL
Mini Filter Spin Columns	50	20	–
Midi Filter Spin Columns	–	–	10
Midi Collection and Elution Tubes	–	–	20
Collection Tubes	50	40	10
Spin Columns	–	20	10
Elution Tubes (1.7 mL)	50	40	10
Product Insert	1	1	1

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Gly-Gly-Gly-PEG4-DBCO

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