TDL5E Bench top low speed refrigerated centrifuge machine 

Overview

• Detection kits for Legionella sp.
• Lyophilized format designed for room temperature shipping
• Available in TaqMan format for analysis

Norgen’s Legionella sp. TaqMan PCR Lyophilized Kit is designed for the detection of Legionella sp. specific DNA in a real-time PCR based on the use of TaqMan® technology. The lyophilized format is designed to ship the kit at ambient temperature.

Norgen’s Legionella sp. TaqMan Lyophilized Probe/Primer and Control Set is designed for the detection of Legionella sp. specific DNA in a real-time PCR based on the use of TaqMan® technology. The lyophilized format is designed to ship the kit at ambient temperature.

Details

Supporting Data

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Storage Conditions and Product Stability

• All kit components should be stored at -20°C upon arrival.
• Once reconstituted, repeated thawing and freezing (>2 times) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.
• Each kit is provided with 4 tubes of 2X PCR Master Mix and each tube is enough to run 25 reactions. It is not necessary to reconstitute all Master Mix tubes at once. The Master Mix tubes can be reconstituted as and when needed.
• All kit components can be stored for 2 years after the date of production without showing any reduction in performance.

• Screening of Cylindrospermopsin in water samples at or above 30ppt
• Format: 15 tests (5 samples at 2 dilutions/5 controls)
• 5 – 10X Sample Dilution Buffer
• 5 – 1X Sample Dilution Buffer
• 5- Negative Control
• 10- Fixed Volume Pipettes

Description

Attogene’ s Cylindrospermopsin Lateral Flow Kit can be used to detect Cylindrospermopsin in source water samples.

Format: Rapid-Water – Run Time: 30 Minutes, enough to run two samples (undiluted and a 10 fold dilution) and negative control.

Cylindrospermopsin (CYN) is a potent cyanotoxin synthesized by select species of cyanobacteria, prominently including Cylindrospermopsin raciborskii. It belongs to the tricyclic alkaloid class, exhibiting a molecular weight of approximately 415 Da. Structurally, cylindrospermopsin features an uracil ring fused with a hydantoin moiety, alongside a guanidino group, attributes that render it highly soluble and polar in aqueous environments.

Cylindrospermopsin is notorious for its profound toxicity towards aquatic organisms and its potential threat to human health through exposure via contaminated water and food sources. Consequently, rigorous monitoring protocols are essential in regions prone to cyanobacterial blooms, where cylindrospermopsin can accumulate in freshwater reservoirs and other aquatic habitats. In recognition of these risks, regulatory bodies such as the United States Environmental Protection Agency (EPA) have implemented an action level guideline. As of 2019, EPA 10-day drinking water health advisory for cylindrospermopsin recommended a threshold of 0.7 parts per billion (ppb), or 700 parts per trillion (ppt) for children under the age of six, and 3 parts per billion, 3000 parts per trillion for anyone older, to effectively manage cylindrospermopsin levels. This precautionary measure aims to uphold both environmental sustainability and public health integrity by minimizing exposure risks.  The EPA has also drafted a human health recreational water quality criterion to protect human health at 8,000ppt.

EPA 10-Day drinking water Health Advisories for Cylindrospermopsin: 

Do not Drink – 0.7 μg/L for bottle fed infants and preschool children, pregnant and nursing woman, elderly immunocompromised and liver conditions

Do not Drink – 3.0 μg/L for school age children to adults

Do Not Use – 20 μg/L

EPA Draft Human Health Recreational Ambient Water Quality Criteria to protect human health: 8 μg/L

Screening of Cylindrospermopsin in water samples at or above 30ppt

Format: 15 tests (5 samples at 2 dilutions/5 controls)

5 – 10X Sample Dilution Buffer

5 – 1X Sample Dilution Buffer

5- Negative Control

10- Fixed Volume Pipettes

The Opentrons Flex HEPA/UV Module enables you to run sensitive contamination-prone applications. It removes 99.99% of 0.3 μm DNA-containing particulates and biological contaminants like bacteria, fungi, and other microorganisms from the air, creating a clean work environment inside the Opentrons Flex.

The Opentrons Flex HEPA/UV Module enables you to run sensitive contamination-prone applications. It removes 99.99% of 0.3 μm DNA-containing particulates and biological contaminants like bacteria, fungi, and other microorganisms from the air, creating a clean work environment inside the Opentrons Flex.