The SMO-HiFi™ DNA Polymerase is a new genetically modified, recombinant DNA polymerase with fidelity 70 times higher than Taq DNA polymerase during amplification, as well as very high elongation rate. Being highly thermostable, SMO-HiFi™ DNA Polymerase can remain viable even after being subjected to boiling for 2 minutes. The SMO-HiFi™ DNA Polymerase is also designed to operate in much lower Mg2+ concentration as compared to other DNA polymerase products.
Detail
Description
The SMO-HiFi™ DNA Polymerase is a new genetically modified, recombinant DNA polymerase with fidelity 70 times higher than Taq DNA polymerase during amplification, as well as very high elongation rate. Being highly thermostable, SMO-HiFi™ DNA Polymerase can remain viable even after being subjected to boiling for 2 minutes. The SMO-HiFi™ DNA Polymerase is also designed to operate in much lower Mg2+ concentration as compared to other DNA polymerase products.
Features
5’→3′ DNA polymerase activity
3’→5′ exonuclease (proofreading) activity
High reaction rate (up to 1 kb/10 seconds)
High fidelity, 70 times higher than Taq DNA polymerase
Blunt end amplicons
Thermo-stable: half-life is more than 10 hrs at 95°C
Storage
[TF1000] SMO-HiFi™ DNA Polymerase
-20°C for 24 months
Other Products
IVD5116 HiPure FFPE DNA/RNA Kit
Product Info
Document
Product Info
Introduction
The Kit is specially designed for simultaneous purification of genomic DNA and total RNA from formalin-fixed, paraffin-embedded (FFPE) tissue sections. The Purified DNA/RNA is used for RT-PCR and PCR detection.
Details
Specifications
Features
Specifications
Main FunctionsC
Co-isolation DNA and RNA from a single FFPE tissue sample
Applications
RT-PCR, cDNA synthesis, PCR and second-generation sequencing, etc.
Purification method
Mini spin column
Purification technology
Silica technology
Process method
Manual (centrifugation or vacuum)
Sample type
FFPE slice, FFPE embedded tissue
Sample amount
No more than six 10µm sections of 150mm2 surface area or three 20µm sections of 150mm2 surface area.
Principle
FFPE samples are incubated in an optimized lysis buffer, which results in the release of RNA and precipitation of DNA. After centrifugation, the RNA-containing supernatant and DNA-containing pellet are then processed separately to purify RNA and DNA. For RNA purification, transfer RNA Lysate to an adsorption column and RNA is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, RNA was finally eluted with low-salt buffer. For DNA purification, transfer DNA Lysate to an adsorption column and DNA is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, DNA was finally eluted with low-salt buffer.
Advantages
1.Use non-toxic dewaxing solution without contact with xylene 2.Obtain both DNA and RNA simultaneously from the same sample. Elute separately without affecting each other (Have the same steps and effects as top brand 80234, perfect substitute.)
Kit Contents
Contents
IVD5116
Purification Times
50 Preps
HiPure DNA Micro Column
50
HiPure RNA Mini Column I
50
2ml Collection Tubes
150
Proteinase K
50 mg
Protease Dissolve Buffer
5 ml
Buffer DPS
60 ml
Buffer FRL
15 ml
Buffer ATL
15 ml
Buffer RLC
15 ml
Buffer AL
15 ml
Buffer VHB
44 ml
Buffer RW2
25 ml
RNase Free Water
10 ml
Buffer AE
10 ml
Storage and Stability
Proteinase K should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
Experiment Data
Document
The Kit is specially designed for simultaneous purification of genomic DNA and total RNA from formalin-fixed, paraffin-embedded (FFPE) tissue sections. The Purified DNA/RNA is used for RT-PCR and PCR detection.
This ELISA kit is intended for the quantitative detection of IgG antibodies against Schistosoma mansoni and Schistosoma haematobium in human serum.
This product is manufactured by Bordier Affinity Products in Switzerland and distributed in Germany exclusively by Milenia Biotec.
Method/Platform
ELISA in microplate format
Range/Assay Sensivity
pNPP, λ=405 nm
Test Principle
Specific antibodies in the sample will bind to Schistosoma mansoni antigens sensitized on microtiter plates. The presence of parasite specific antibodies is detected with a Protein A alkaline phosphatase conjugate.
12 x 8 strips (96 tests)
This ELISA kit is intended for the quantitative detection of IgG antibodies against Schistosoma mansoni and Schistosoma haematobium in human serum.
Isolate total DNA and RNA from all microorganisms found in water, including bacteria, fungi and algae
RNA and DNA are both column purified simultaneously using the same column
Elution contains concentrated DNA and RNA without the need for further precipitation
Complete RNA (including microRNA) without phenol
Isolated RNA and DNA are of high quality and integrity for all downstream applications
Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix
These kits provide a convenient and rapid method for the isolation of microorganisms from water samples. The kits allows for the rapid isolation and purification of total RNA and DNA simultaneously from the microorganisms found in different types of water samples. The total RNA and DNA (including genomic DNA) are isolated from all the microorganisms found in the water, including bacteria, fungi and algae without the use of any inhibitory organic substances The kit purifies genomic DNA, and all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA). The purified RNA and DNA are highly concentrated, and can be used directly in a number of downstream applications including PCR, qPCR, RT-PCR, qRT-PCR, Northern blotting, Southern blotting and sequencing reactions.
Water RNA/DNA Purification Kit (Spin Column + Filters Kits)
These kits are available with 0.45 μm or 0.22 μm filter formats for capturing microorganisms present in the water.
Storage Conditions and Product Stability All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years from the date of shipment.