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[TF1000] SMO-HiFi™ DNA Polymerase, 1 U/μl, 100 U

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The SMO-HiFi™ DNA Polymerase is a new genetically modified, recombinant DNA polymerase with fidelity 70 times higher than Taq DNA polymerase during amplification, as well as very high elongation rate. Being highly thermostable, SMO-HiFi™ DNA Polymerase can remain viable even after being subjected to boiling for 2 minutes. The SMO-HiFi™ DNA Polymerase is also designed to operate in much lower Mg2+ concentration as compared to other DNA polymerase products.

Detail

Description 

The SMO-HiFi™ DNA Polymerase is a new genetically modified, recombinant DNA polymerase with fidelity 70 times higher than Taq DNA polymerase during amplification, as well as very high elongation rate. Being highly thermostable, SMO-HiFi™ DNA Polymerase can remain viable even after being subjected to boiling for 2 minutes. The SMO-HiFi™ DNA Polymerase is also designed to operate in much lower Mg2+ concentration as compared to other DNA polymerase products. 

Features

  • 5’→3′ DNA polymerase activity
  • 3’→5′ exonuclease (proofreading) activity 
  • High reaction rate (up to 1 kb/10 seconds)
  • High fidelity, 70 times higher than Taq DNA polymerase 
  • Blunt end amplicons
  • Thermo-stable: half-life is more than 10 hrs at 95°C 

Storage

[TF1000] SMO-HiFi™ DNA Polymerase

-20°C for 24 months

Other Products

Plasma/Serum Exosome and Free-Circulating RNA Isolation Kits

Overview

  • Isolate all sizes of RNA, including microRNA, irrespective of size or GC content, without bias.
  • ​Versatile sample input ranges
  • Isolate all sizes of free-circulating RNA, including microRNA
  • The purified exosomal RNA is free from any circulating RNA-binding proteins
  • No phenol extractions, Proteinase K treatment, nor carrier RNA
  • No time-consuming ultracentrifugation, filtration nor special syringes are required
  • No precipitation reagents, nor overnight incubation required
  • Concentrate isolated exosomal RNA and are free-circulating RNA into a flexible elution volume ranging from 50 µL to 100 µL
  • Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

These kits provide a fast, reliable and convenient method to sequentially isolate and concentrate exosomal RNA as well as Free-Circulating RNA from different plasma/serum sample volumes. The purification is based on spin column chromatography that employs Norgen’s proprietary resin. These kits are designed to isolate all sizes of RNA, including microRNA as well as all sizes of the free-circulating protein-bound RNA, including microRNA. These kits provide a clear advantage over other available kits in that they do not require any special instrumentation, protein precipitation reagents, extension tubes, phenol/chloroform or protease treatments. Moreover, these kits allow the user to elute into a flexible elution volume ranging from 50 µL to 100 µL. The RNA isolated from the purified exosomes is free from any protein-bound circulating RNA and is of the highest integrity. Moreover, the free-circulating, protein-bound, RNA is free from any exosomal RNA. The purified RNA can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.

Plasma/Serum Exosome and Free-Circulating RNA Isolation Mini Kit

For sample volumes ranging from 50 µL to 1 mL.

Plasma/Serum Exosome and Free-Circulating RNA Isolation Midi Kit

For sample volumes ranging from 1 mL to 4 mL.

Plasma/Serum Exosome and Free-Circulating RNA Isolation Maxi Kit

For sample volumes ranging from 4 mL to 10 mL.

Details

Supporting Data

Figure 1 / 6

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Figure 1. Plasma/Serum Exosome and Free-Circulating RNA Isolation Mini Kit
Figure 1. Plasma/Serum Exosome and Free-Circulating RNA Isolation Mini Kit
Figure 2. Plasma/Serum Exosome and Free-Circulating RNA Isolation Mini Kit
Figure 3. Plasma/Serum Exosome and Free-Circulating RNA Isolation Midi Kit
Figure 4. Plasma/Serum Exosome and Free-Circulating RNA Isolation Midi Kit
Figure 5. Plasma/Serum Exosome and Free-Circulating RNA Isolation Maxi Kit
Figure 6. Plasma/Serum Exosome and Free-Circulating RNA Isolation Maxi Kit

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Kit Specifications
Minimum Plasma Input4 mL
Maximum Plasma Input10 mL
Size of RNA PurifiedAll sizes, including miRNA
and small RNA (< 200 nt)
Elution Volume50 – 100 µL
Time to Complete 10 Purifications40 – 45 minutes
Average Yields*Variable depending on specimen

*Please check page 5 of the product insert for the average yields and the common RNA quantification methods

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.

Important Note
This kit is suitable for the purification of exosomes from fresh or frozen serum or plasma prepared from blood collected on either EDTA or Citrate. Plasma samples prepared from blood collected on heparin should not be used as heparin can significantly interfere with many downstream applications such as RT-PCR.

ComponentCat. 59500 (50 preps)Cat. 59600 (25 preps)Cat. 59700 (15 preps)
Slurry E12.5 mL12.5 mL12.5 mL
ExoC Buffer8 mL8 mL2 x 8 mL
ExoR Buffer12 mL12 mL12 mL
Lysis Buffer A2 x 20 mL2 x 20 mL2 x 20 mL
Lysis Additive B2 mL2 mL2 mL
Wash Solution A2 x 18 mL18 mL18 mL
Elution Solution A2 x 6 mL6 mL6 mL
Mini Filter Spin Columns502515
Mini Spin Columns1005030
Collection Tubes1005030
Elution Tubes (1.7 mL)1005030
Product Insert111

DBCO-PEG4-PFP ester

DBCO-PEG4-PFP ester is a PFP active ester containing a DBCO group which can undergo copper-free Click Chemistry with azide (N3). The PFP ester moiety is reactive with amine groups. PFP esters have been are stable compounds and are less susceptible to undergo hydrolysis. The hydrophilic PEG linker increases the water solubility of a compound in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

endo-BCN-PEG3-NHS ester

endo-BCN-PEG3-NHS esterr can be used to label the primary amines (-NH2) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. The BCN group enable copper free click chemistry with azide-tagged biomolecules. The hydrophilic PEG spacer increases solubility in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.