The G-HiFi™ DNA Polymerase is a new genetically modified, recombinant DNA polymerase suitable for GC-rich templates that are difficult to amplify. The fidelity of G-HiFi™ DNA Polymerase is 70 times higher than that of Taq DNA polymerase. The high extension rate of G-HiFi™ DNA Polymerase is achieved by blending the DNA polymerase with an elongation enhancer. The optimized 5X G-HiFi™ Buffer includes special ingredients that suppress non-specific amplification as well as plateau effect produced by conventional PCR. With the optimized 5X G-HiFi™ Buffer, G-HiFi™ DNA Polymerase is capable to amplify most templates, such as longer targets (up to 40 kb from lambda DNA) and that contain GC-rich sequences.
Detail
Description
The G-HiFi™ DNA Polymerase is a new genetically modified, recombinant DNA polymerase suitable for GC-rich templates that are difficult to amplify. The fidelity of G-HiFi™ DNA Polymerase is 70 times higher than that of Taq DNA polymerase. The high extension rate of G-HiFi™ DNA Polymerase is achieved by blending the DNA polymerase with an elongation enhancer. The optimized 5X G-HiFi™ Buffer includes special ingredients that suppress non-specific amplification as well as plateau effect produced by conventional PCR. With the optimized 5X G-HiFi™ Buffer, G-HiFi™ DNA Polymerase is capable to amplify most templates, such as longer targets (up to 40 kb from lambda DNA) and that contain GC-rich sequences.
Features
5’→3’ DNA polymerase activity
3’→5’ exonuclease (proofreading) activity
Suitable for GC-rich templates
High reaction rate: 7 seconds/kb
High fidelity: 70 times higher than Taq polymerase
Our SNPsig® kits use our own proprietary genotyping method to enable the identification of SARS-CoV-2 variants of concern. These products can be used on any real-time PCR machine using familiar protocols, whilst resulting in exceptional genotyping data.
Positive control templates for wild-type and variants are supplied in every kit to make data interpretation simple.
Our SNPsig® technology provides an alternative to sequencing as well as S gene target failure (SGTF) that enables scientists to analyse and monitor these specific genomic mutations. Our kits can provide a pivotal role in screening for SARS-CoV-2 variants for the purpose of genomic surveillance and studies.
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For the detection of the SARS-CoV-2 (20I/501Y.V1 UK)
Rapid detection of specific detection profiles
High priming efficiency
Sensitive to < 100 copies of target
Positive copy number standard curve for quantification
Accurate controls to confirm findings
96 reactions, includes master mix
The BK virus is a member of the polyomavirus family. It has been suggested that this virus may be transmitted through respiratory fluids or urine, since infected individuals periodically excrete virus in the urine. BK viral infections are typically asymptomatic in healthy individuals, however very mild symptoms may appear including mild respiratory infections and fever. Infections with BK virus in immunocompromised or immunosupressed patients are much more severe and may involve renal dysfunction. In fact, in kidney transplant patients the immunosupressive drugs required for the transplant may allow the virus to replicate within the graft, resulting in a disease called BK virus nephropathy (BKVN). The JC virus is a type of human polyomavirus and is very common in the general population, infecting 70 to 90% of humans. Most people acquire JCV in childhood or adolescence. Typically the infection is subclinical and no of consequence in individuals with healthy immune systems. The initial site of infection may be the tonsils or the gastrointestinal tract, and the virus then remains latent in the gastrointestinal tract. JCV can also infect the tubular epithelial cells in the kidneys, where it continues to reproduce, shedding virus particles in the urine. Also, JCV can cross the blood-brain barrier into the central nervous system. JCV is known to cause the usually fatal progressive multifocal leukoencephalopathy (PML) by destroying oligodendrocytes in the brain in immunodeficient or immunosuppressed individuals. The JC and BK viruses are very similar, with their genomes sharing 75% homology. It is however important to differentiate between the viruses due to the differences in pathology and especially the invariably fatal outcome of PML which is only caused by the JC virus.
BKV/JCV TaqMan PCR Kit, 100 reactions
Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
Specific Primer and Probe mix for the pathogen/virus/viroid of interest
Primer and Probe mix
Positive and negative control to confirm the integrity of the kit reagents
BKV/JCV TaqMan PCR Probe/Primer Set and Controls, 100 reactions
Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest
Nuclease-free water
Can be used together with Norgen’s PCR Master Mix (#28007) or customer supplied master mix
For research use only and NOT intended for in vitro diagnostics.
Storage Conditions and Product Stability All kit components can be stored for 2 years after the date of production without showing any reduction in performance.
All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.
Accurate & Reliable Testing Solutions Diagnosis Nucleic Acid Detection White Spot Syndrome Virus
Product Description:
The Livestock Disease Kit is a reliable and easy-to-use test kit designed to detect the presence of diseases in livestock, such as cattle, sheep, goats, pigs and horses. The kit has been designed to detect diseases with high accuracy and sensitivity. Also it requires only a small sample volume of 50 μl, making it highly efficient and cost-effective. It is also suitable for a range of canine dog test kit applications, providing fast and accurate results.The reliable detection results make it a reliable and cost-effective solution for farmers and veterinarians looking to diagnose livestock diseases quickly and accurately.
Features:
Product Name: White spot syndrome virusisothermal detetction fluorescence kit
Target Species: Shrimp
Type: Fluorescence Kit
Storage Temperature: -20°C
Sample Type: Serum, Plasma, Whole Blood
Technical Parameters:
Product Name
White spot syndrome virus isothermal detetction fluorescence kit
Type
Fluorescence Kit
Sample Volume
50 μl
Sample Type
Serum, Plasma, Whole Blood
Storage Temperature
-20°C
Package Size
48 Tests/Kit
Target Disease
Livestock Disease
Target Species
Shrimp
Applications:
Amp-future bio Livestock Disease Kit is an innovative animal health care product designed to detect and diagnose livestock diseases. Developed by a Chinese research team, this kit is a simple and reliable solution to test and treat livestock diseases. With its detection limit of 0.1 ng/ml, users can obtain accurate results quickly and accurately. The test time takes from 5-20 minutes, and the kit typically contains 48 tests/kit. Furthermore, it is easy to store with its cold storage temperature of -20°C.
The Amp-future bio Livestock Disease Kit provides an innovative solution for animal health care. It is an essential product for veterinary professionals, pet owners, and animal health researchers to detect and diagnose livestock diseases. With its simple and reliable detection and diagnosis, this kit is a must-have for anyone who wants to ensure the health of their animals.
Support and Services:
Livestock Disease Kit Technical Support and Services
We provide technical support and services for our Livestock Disease Kit. We are committed to helping you get the most out of your product and ensuring your satisfaction with our products and services.
Our technical support staff is available to answer any questions you may have about the product.
We offer online tutorials and user guides to help you understand the product better.
We provide technical assistance to help troubleshoot any issues you may be having with the product.
We offer a warranty on our products.
If you have any questions or need technical support, please do not hesitate to contact us. We are here to help you make the most of your Livestock Disease Kit.
FAQ:
Q: What diseases can Livestock Disease Kit detect? A: Livestock Disease Kit can detect different infectious diseases, such as Foot and Mouth Disease, Bluetongue Disease, Bovine Viral Diarrhea.
Q: How does Livestock Disease Kit work? A: Livestock Disease Kit uses advanced MIRA technology to amplify the target DNA/RNA targent sequences , and detect the disease by analyzing the amplified sequences.
Q: How long does it take to get results from Livestock Disease Kit? A: Livestock Disease Kit can give a result within 5-20 mins.
Q: What type of sample is suitable for Livestock Disease Kit? A: Livestock Disease Kit is suitable for blood, serum, and tissue samples.
Q: Does Livestock Disease Kit require professional equipment? A: No, Livestock Disease Kit does not require any professional equipment,can use with our isothermal equipment or pcr machine
Document
The Livestock Disease Kit is a reliable and easy-to-use test kit designed to detect the presence of diseases in livestock, such as cattle, sheep, goats, pigs and horses. The kit has been designed to detect diseases with high accuracy and sensitivity. Also it requires only a small sample volume of 50 μl, making it highly efficient and cost-effective. It is also suitable for a range of canine dog test kit applications, providing fast and accurate results.The reliable detection results make it a reliable and cost-effective solution for farmers and veterinarians looking to diagnose livestock diseases quickly and accurately.