[TP1000] ExcelTaq™ Taq DNA Polymerase, 5 U/μl, 500 U
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ExcelTaq™ Taq DNA Polymerase is a recombinant thermo-stable Taq DNA polymerase expressed and purified from an E. coli strain carrying the cloned gene. With a high DNA synthesis rate and high thermo-stability, ExcelTaq™ Taq DNA Polymerase is suitable for common and specialized PCR applications.
Detail
Description
ExcelTaq™ Taq DNA Polymerase is a recombinant thermo-stable Taq DNA polymerase expressed and purified from an E. coli strain carrying the cloned gene. With a high DNA synthesis rate and high thermo-stability, ExcelTaq™ Taq DNA Polymerase is suitable for common and specialized PCR applications.
Features
5’→3′ DNA polymerase activity
5’→3′ exonuclease activity
No detectable 3’→5′ exonuclease (proofreading) activity
Generates PCR products with 3’-dA overhangs
Thermo-stable – half-life lasts for more than 40 min at 95°C
Applications
Routine PCR
Amplification of DNA fragments up to 8 kb
Generation of PCR products for TA cloning
DNA labeling
Storage
-20°C for 24 months
Other Products
IVD3141 HiPure Stool DNA Kit
Product Info
Document
Product Info
Introduction
With the development of molecular biology, stool, a new non-invasive sample, has been widely used in the research of animal molecular genetics, population ecology, behavioral ecology and some intestinal disease diagnosis. Stool samples includes gut microbial DNA, food residue sample DNA, and alimentary tract exfoliated cell DNA.
The primary problem encountered when using stool sample for molecular biology research is the low content of exfoliated cells in the digestive tract and a certain degree of degradation of genetic material in stool. Another issue in molecular scatology research based on PCR is the presence of a large number of inhibitors in stool that can affect Taq enzyme activity, leading to downstream detection inactivation. These inhibitors include polysaccharides, plant polysaccharides, bile acids, bile salts, bile pigments, digestive juices, mucus, etc. Therefore, selecting appropriate extraction methods to obtain high-quality DNA is the key to successful downstream detection of stool DNA.
At present, the pretreatment methods used in the laboratory, such as phenol/chloroform extraction, cetyltrimethyl bromide (CTAB) lysis, and guanidine isothiocyanate lysis, lack universality in different species, and the success rate of extracting DNA for PCR amplification is also very low. The HiPure Stool DNA Kit provided by Magen Company has opened up a new approach for DNA extraction from stool samples with good universality, high cost-effectiveness, high yield and purification. The reagent kit adopts a unique solution system and inhibitory factor adsorbent, which can efficiently remove various impurities in stool samples. The purified DNA can be directly used for PCR, quantitative PCR and other applications.
This product allows rapid and reliable isolation of high-quality genomic DNA from various stool samples. Up to 100 mg soil samples can be processed in 60 minute. The system combines the reversible nucleic acid binding properties of HiPure matrix with the speed and versatility of spin column technology to eliminate PCR inhibiting compounds such as humic acid from soil samples. Purified DNA is suitable for PCR, restriction digestion, and next-generation sequencing. There are no organic extractions thus reducing plastic waste and hands-on time to allow multiple samples to be processed in parallel.
Details
Specifications
Features
Specifications
Main Functions
Isolation total DNA from 50-100mg stool samples
Applications
PCR, Southern Blot, enzyme digestion and NGS, etc.
Purification method
Mini spin column
Purification technology
Silica technology
Process method
Manual (centrifugation or vacuum)
Sample type
Stool
Sample amount
50-100mg
Yield
3-15μg
Elution volume
≥30μl
Time per run
≤60 minutes
Liquid carrying volume per column
750μl
Binding yield of column
100μg
Principle
Stool sample is homogenized and then treated in a specially formulated buffer containing detergent to lyse bacteria, yeast, and fungal samples. Humic acid, proteins, polysaccharides, and other contaminants are removed using our proprietary Absorber Solution. Binding conditions are then adjusted and the sample is applied to a DNA Mini Column. Two rapid wash steps remove trace contaminants and pure DNA is eluted in low ionic strength buffer. Purified DNA can be directly used in downstream applications without the need for further purification.
Advantages
High purity – unique adsorbent can completely remove inhibitory factors
High concentration – maximum extraction of total DNA from stool samples
High recovery – DNA can be recovered at the level of PG
Good repeatability – silica technology can obtain ideal results every time
Kit Contents
Contents
IVD3141
Purification Times
50 Preps
HiPure DNA Mini Columns II
50
2ml Collection Tubes
50
2ml Bead Tubes
50
Proteinase K
24 mg
Protease Dissolve Buffer
1.8 ml
Buffer SPL
40 ml
Buffer PCI
40 ml
Buffer AL
20 ml
Buffer GW1
22 ml
Buffer GW2
20 ml
Buffer AE
15 ml
Storage and Stability
Proteinase K and Buffer PCI should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2–8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.
Document
With the development of molecular biology, stool, a new non-invasive sample, has been widely used in the research of animal molecular genetics, population ecology, behavioral ecology and some intestinal disease diagnosis. Stool samples includes gut microbial DNA, food residue sample DNA, and alimentary tract exfoliated cell DNA.
This product is suitable for rapid extraction of DNA from FFPE sample, tissue, cells, blood, swabs, blood spots, semen and other clinical samples. This product uses size selection magnetic beads, which can selectively remove small sizes of DNA (100-300bp) from FFPE samples by adjusting the amount of binding solution, so as to improve the effective data volume of downstream NGS.
Details
Specifications
Features
Specifications
Main Functions
Isolation total DNA from FFPE using high bind beads
Applications
RT-PCR, northern blot, poly A purification, nucleic acid protection and in vitro translation, etc.
Purification technology
Magnetic beads technology
Process method
Manual or automatic
Sample type
Large quantities of solids
Sample amount
Appropriate
Elution volume
≥50μl
Time per run
30 – 120 minutes
Principle
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by Elution Buffer.
Advantages
1.Use non-toxic dewaxing solution without contact with xylene
2.Efficient removal of formaldehyde modification on DNA and improvement of PCR sensitivity
3. One of the best FFPE DNA extraction kits on the market, the same effect of paraffin slice extraction as top brand, and the effect of puncture sample extraction is even better than top brands
Kit Contents
Contents
D632301B
D632302B
Purification Times
48 Preps
96 Preps
MagBind Particles
1.1 ml
2 x 1.1 ml
RNase A
10 mg
20 mg
Proteinase K
24 mg
48 mg
Protease Dissolve Buffer
3 ml
6 ml
Buffer DPS
60 ml
100 ml
Buffer ATL
15 ml
30 ml
Buffer AL
15 ml
30 ml
Buffer BD*
6 ml
15 ml
Buffer BXW1*
13 ml
44 ml
Elution Buffer
15 ml
30 ml
Storage and Stability
RNase A, Proteinase K and MagBind Particles should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
Experiment Data
Document
This product is suitable for rapid extraction of DNA from FFPE sample, tissue, cells, blood, swabs, blood spots, semen and other clinical samples. This product uses size selection magnetic beads, which can selectively remove small sizes of DNA (100-300bp) from FFPE samples by adjusting the amount of binding solution, so as to improve the effective data volume of downstream NGS.
This kit is based on a rapid nucleic acid amplification technology at room temperature and constant temperature: at room temperature and constant temperature (generally 39ºC~42ºC), with the help of auxiliary proteins and single-strand binding proteins,the recombinase and primers form a complex; Source search and combine the target homology domain, at this time, a D-loop region is formed at the homology position and strand exchange begins;along with the dissociation of the recombinase from the complex,the polymerase also binds to the 3′ end of the primer and begins chain extension.It is suitable for laboratory-level DNA amplification and DNA amplification for other detection purposes.