Description
The ExcelTaq™ Hot Start II DNA Polymerase is a mixture of an aptamer-based inhibitor and a recombinant thermo-stable Taq DNA polymerase designed for preventing or minimizing non-specific DNA amplification in PCR reaction. The inactivation of polymerase is achieved by a reversible binding of the aptamer to the polymerase at temperatures below 45°C. The aptamer inhibitor releases polymerase during normal PCR cycling. The aptamer-based inhibition omits the time-consuming initial activation step required by chemically modified or antibody-based hot start polymerases.
The high specificity and sensitivity of ExcelTaq™ Hot Start II DNA Polymerase allows sensitive detection from limited amount of DNA templates, such as 1 pg of cDNA or 1 fg of plasmid DNA. With a high DNA synthesis rate and high thermo-stability, the ExcelTaq™ Hot Start II DNA Polymerase allows reactions to be set up at room temperature and is suitable for common and specialized PCR applications
Features
- Aptamer-based hot start PCR
- Reversible enzyme inactivation
- Omits extra enzyme activation step
- Convenient for room temperature PCR set-up
- High yield and specificity of target amplicons
- Wide range of amplicon length (up to 10 kb)
- High sensitivity (as low as 1 fg of plasmid)
Applications
- High specificity PCR
- Generation of PCR products for TA cloning
- Routine PCR, multiplex PCR, colony PCR, and RT-PCR
Storage
-20°C for 24 months
