The ExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX) is a ready-to-use reagent with all the essential components for quantitative real-time PCR (qPCR) except primers and template. The master mix features high sensitivity and signal intensity as well as low background and better compatibility with cDNA templates derived directly from reverse transcription reaction mixture. The ExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX) contains hot-start Taq polymerase in an optimized buffer with dsDNA specific SYBR green fluorescent dye. This master mix allows for sensitive, precise amplification, real-time tracking of the amplification process, and simultaneous quantification for targeted DNA molecules. With inert smart blue contrast dye, the ExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX) is ready-to-use and greatly reduces pipetting errors, while largely improving the reproducibility of the process. The ExcelTaq™ 2X Q-PCR Master Mix is also compatible with a ROX reference dye if recommended by the manufacturer of the qPCR system.
Detail
Description
The ExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX) is a ready-to-use reagent with all the essential components for quantitative real-time PCR (qPCR) except primers and template. The master mix features high sensitivity and signal intensity as well as low background and better compatibility with cDNA templates derived directly from reverse transcription reaction mixture. The ExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX) contains hot-start Taq polymerase in an optimized buffer with dsDNA specific SYBR green fluorescent dye. This master mix allows for sensitive, precise amplification, real-time tracking of the amplification process, and simultaneous quantification for targeted DNA molecules. With inert smart blue contrast dye, the ExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX) is ready-to-use and greatly reduces pipetting errors, while largely improving the reproducibility of the process. The ExcelTaq™ 2X Q-PCR Master Mix is also compatible with a ROX reference dye if recommended by the manufacturer of the qPCR system.
Features
High sensitivity and signal intensity
Smart blue contrast dye as a visual aid for reaction setup
Better compatibility for reverse transcription
Low background
Storage
Protected from light. Aliquot to avoid multiple freeze-thaw cycles. -20°C for 12 months
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Product Info
Document
Product Info
Introduction
Usages:
For differentiation of enterobacteriaceae.
Principle:
Peptone, beef extract powder and yeast extract powder provide nitrogen, vitamins, minerals; lactose, dextrose into fermentable sugars, which produce acid when measured by the phenol red indicator, acid yellow, basic red; thiosulfate sodium can be reduced to some bacteria to hydrogen sulfide, to produce a black iron sulfide and iron salts; sodium chloride to maintain osmotic equilibrium; agar as medium coagulant.
Formulation(per liter):
Peptone 20g
Beef extract powder 3g
Yeast extract powder 3g
Lactose 10g
Dextrose 1g
Sodium chloride 5g
Ferric ammonium citrate 0.5g
Sodium thiosulfate 0.5g
Agar 12g
Phenol red 0.05g
Final pH 7.4 ± 0.2
How to use:
1.Suspend 54.5g in 1L of distilled water , stirring heated to boiling ,autoclave at 121 for 15 minutes.
2.Diluted and treated samples.
Quality control:
Item
The name and number of strain
Growth
Colony Color
1
Escherichia coli ATCC25922
Good
A/A
2
Proteus CMCC (B) 49027
Good
K/A
3
Salmonella typhimurium CMCC (B) 50115
Good
K/A
“A”Acid , “K” Alkality
Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.
1. Brushless motor with simpler construction, more reliable performance, longer life and quietly running. 2. Automatically electric lid lock, super speed, over temperature protection and imbalance protection. The centrifuge body is made of high quality steel, safe and reliable. 3. Rotor is connected to spindle by specialized taper sleeve, loading simple and quick, no direction, safe and reliable. 4. Microprocessor control, TD5B with digital display which indicate the speed, time, RCF in operation, speed raising and reducing quick, operate simply. 5.10 kinds of accelerating and decelerating speed for your choice.
TD5B Technical Parameter：
Max. Speed
5000rpm
Max. RCF
4730×g
Max. Capacity
4×800ml
Time Range
0~99min
RPM/RCF Convert
Yes
Noise (dB)
≤ 58
Temperature
Normal
Acc/Dec
10 Kinds
Speed Accuracy
±20r/min
Temperature Accuracy
/
Voltage(V/Hz)
AC 220V/110V 50HZ/60HZ
Size (W x D x Hmm)
685×500×385mm
Net Weight(Kg)
67KG
Certificates
CE,ISO & Calibration report are available
Matched Rotors for TD5B
Order No
Rotor
Max Speed(r/min)
Volume(ml)
Max RCF(×g)
5B-1
Swing Rotor
4000
4×800ml
3450
5B-2
Swing Rotor
4000
4×750ml
3530
5B-3
Swing Rotor
4000
4×500ml
3380
5B-4
Swing Rotor
4000
4×4×96(well)
2940
5B-5
Microplate rotor
4000
2×4×96(well)
3210
5B-6
2×3×48(well)
2300
5B-7
Swing rotor
5000
4×1×50ml
4730
4×1×100ml
4730
4000
4×2×100ml
3020
4000
4×2×50ml
3200
4000
4×4×15ml
3200
4000
4×6×15ml
3200
5B-8
Swing rotor
4000
4×8×15ml
3200
4000
4×12×5ml vacuum tube
2480
4000
4×12×7ml vacuum tube
2760
4000
4×12×10ml vacuum tube
2880
4000
4×8×10/7/5ml vacuum tube
2790
4000
4×6×10/7/5ml vacuum tube
2790
4000
4×4×10/7/5ml vacuum tube
2790
5B-9
Swing rotor
4000
4×24×5ml vacuum tube
2480
4000
4×31×5ml Khan tubes
2480
5B-10
Swing rotor
4000
4×24×7ml vacuum tube
3140
4000
4×31×7ml Khan tubes
3140
4000
4×30×7ml vacuum tube
3140
4000
4×30×5ml vacuum tube
3140
4000
4×40×7ml vacuum tube
3270
4000
4×18×10ml vacuum tube
3140
5B-11
Swing rotor
4000
Dairy bottle
3830
5B-12
Fixed rotor
5000
6×15ml
2540
5B-13
5000
12×15ml
3080
5B-14
5000
24×15ml
3500
5B-15
5000
30×15ml
3830
5B-16
5000
4×50ml
2520
5B-17
5000
6×50ml
2850
5B-18
5000
12×50ml
3860
5B-19
4000
24×50ml
2970
5B-20
5000
4×100ml
2630
5B-21
5000
6×100ml
3130
5B-22
4000
12×100ml
2970
Adapter for 800ml and 500ml bucket
VolumeCup
5ml
7ml
10ml
15ml
50ml
800ml（Round Cup）
23pcs/cup
23
19
14
5
500ml（Round Cup）
18pcs/cup
18
14
9
3
500ml（Square Cup）
20pcs/cup
20
16
12
5
Document
TD5B large capacity centrifuge max capacity can be reached to 4x800ml, can with different adapters for small tubes, 50ml, 15ml, 10ml, 7ml, 5ml, 2ml and so on.
This product is suitable for extracting total RNA from 50-150mg conventional plantor fungal samples as well as samples rich in polyphenolic and polysaccharides. This kit is based on silica gel column purification technology, and the extraction process does not require the use of toxic phenol chloroform extraction. The entire extraction process only takes 20-30 minutes. This kit adopts DNA filtration technology, which can efficiently filter and remove DNA. The obtained RNA can be directly used for experiments such as RT-PCR, Northern Blot, poly A+ purification, nucleic acid protection, and in vitro translation.
Details
Kit Contents
Contents
R415002
D415003
Purification Times
50 Preps
250 Preps
HiPure RNA Mini Columns
50
250
gDNA Filter Columns
50
250
2ml Collection Tubes
100
500
TCEP (1M)
0.29 g
5 x 0.29 g
Buffer EP
1.0 ml
5.0 ml
Buffer PSL
50 ml
250 ml
Buffer RW1
50 ml
250 ml
Buffer RW2*
20 ml
2 x 50 ml
RNase Free Water
10 ml
30 ml
Storage and Stability
Except TCEP, the kit components can be stored dry at room temperature (15–25°C) and are stable for at least 18 months under these conditions. After receiving the product, it is recommended to store TCEP (dry powder) at -20-8°C. At low temperatures, Buffer PSL may form precipitates, dissolve it completely by 55°C water bath.
Purchase Guide
1. When dealing with woody or uncommon samples, R4150 is recommended first. R4150 contains two polysaccharide/polyphenol lysis buffer, which is the most universal product.
2. R4151 is recommended for handling common economic crop samples for the first time. Strong lysis solution can be used to process easy-extraction samples. The amount of corn or rice leaves samples can reach up to 300mg.
3. R4165 adopts CTAB/chloroform method, which can also handle a large number of difficult-to-extraction plants, but requires contact with chloroform substitutes, which is less safe than other kits. This kit uses DNase Ⅰ to remove DNA, which is also a good choice for extracting polysaccharide/polyphenol-rich plant samples.
4. R4014 is recommended for fruit/starch plant samples, which uses improved trizol pre-treatment, single column operation and is more economical.
Document
This product is suitable for extracting total RNA from 50-150mg conventional plantor fungal samples as well as samples rich in polyphenolic and polysaccharides. This kit is based on silica gel column purification technology, and the extraction process does not require the use of toxic phenol chloroform extraction. The entire extraction process only takes 20-30 minutes. This kit adopts DNA filtration technology, which can efficiently filter and remove DNA. The obtained RNA can be directly used for experiments such as RT-PCR, Northern Blot, poly A+ purification, nucleic acid protection, and in vitro translation.
Features: