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[TQ1201] ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX), 500 RXN

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The ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX) is a ready-to-use reagent with all the essential components for quantitative real-time PCR (qPCR) except primers and templates. The master mix contains a highly stable hot-start Taq polymerase in an optimized buffer with dsDNA specific SYBR green fluorescent dye. Consequently, the ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX) features high stability during storage, even at 37°C for weeks. In addition to high sensitivity and signal intensity, the ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX) performs a low background/ high specificity qPCR results, as well as a better compatibility with fast PCR program.

With inert smart blue contrast dye, the ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX) is ready-to-use and greatly reduces pipetting errors, while largely improving the reproducibility of the process. The ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX) is also compatible with ROX reference dye if ROX is recommended by the manufacturer of the qPCR system. This master mix allows sensitive, precise amplification, real-time tracking of the amplification process, and simultaneous quantification for targeted DNA molecules.

Detail

Description 

The ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX) is a ready-to-use reagent with all the essential components for quantitative real-time PCR (qPCR) except primers and templates. The master mix contains a highly stable hot-start Taq polymerase in an optimized buffer with dsDNA specific SYBR green fluorescent dye. Consequently, the ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX) features high stability during storage, even at 37°C for weeks. In addition to high sensitivity and signal intensity, the ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX) performs a low background/ high specificity qPCR results, as well as a better compatibility with fast PCR program. 

With inert smart blue contrast dye, the ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX) is ready-to-use and greatly reduces pipetting errors, while largely improving the reproducibility of the process. The ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX) is also compatible with ROX reference dye if ROX is recommended by the manufacturer of the qPCR system. This master mix allows sensitive, precise amplification, real-time tracking of the amplification process, and simultaneous quantification for targeted DNA molecules.

Features

  • High Stability
  • Fast Hot Start
  • High Sensitivity
  • Low Background
  • Suitable for Fast Program 
  • Smart Blue Contrast Dye 

Storage

Protect from light.
Aliquot to avoid multiple freeze-thaw cycles. 

-20°C for 12 months

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IST-122 QuickSeal qOpticTM Self Adhesive Sealing Film

Overview

Adhesive film with 96 optically clear windows. This seal is peelable, suitable for qPCR and optical applications.

  • These unique seals combine the strong sealing integrity of our QuickSeal PCR Seal with improved optical properties, thanks to the 96 adhesive-free windows
  • The seal is made of a durably transparent polyester film, and a strong adhesive is applied across the seal, apart from the 96 round windows
  • The QuickSeal qOptic seal is recommended for qPCR and other optical applications, such as fluorescence or colorimetric measurements
  • For all adhesive seals, the best sealing results are achieved using our Hand Roller or KAPS 500 Auto Sealer

Listeria monocytogenes TaqMan PCR Detection Kit Dx

Overview

  • Detection kit for Listeria monocytogenes
  • CE-IVDD marked in accordance with the European Commission Directive 98/79/EC.
  • Research version available
  • Available in TaqMan Probe format for analysis.

Listeria monocytogenes have emerged as significant foodborne pathogens that pose a serious public health problem. As the causative agent of Listeriosis, L. monocytogenes has the highest rate of fatality rate among all foodborne pathogens. L. monocytogenes is a facultatively intracellular, Gram-positive bacterium that could survive high and low temperatures, low pH. It is a rare causative agent of mastitis in cow. However, due to its ability to resist various food processing technologies as well as to grow at low temperature, L. monocytogenes is know to be associated with both raw and pasteurized milk, as well as dairy products such as cheese. As little as 1000 organisms may cause the disease with pregnant, new-born, and immunocompromised individuals the most susceptible.

Listeria monocytogenes TaqMan PCR Kit, 24 reactions

  • Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
  • Specific Primer and Probe mix for the pathogen/virus/viroid of interest
  • Primer and Probe mix
  • Positive and negative control to confirm the integrity of the kit reagents

Details

Supporting Data

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Storage Conditions

The Listeria monocytogenes TaqMan PCR Kit Dx is shipped on dry ice. The components of the kit should be frozen upon arrival. If one or more of the components is not frozen when the kit is received, or if any of the components have been compromised during shipment, please contact Norgen Biotek for assistance. All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 3 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.

ComponentCat. DxTM30400 (24 rxns)
MDx TaqMan 2X PCR Master Mix Dx550 µL
L. monocytogenes Primer Mix Dx2 x 70 µL
L. monocytogenes Positive Control Dx – 200,000 copies/μL50 µL
Nuclease-Free Water2 x 1.25 mL
Product Insert1