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[TQ1211] ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, ROX), 500 RXN

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The ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, ROX) is a ready-to-use reagent with all the essential components for quantitative real-time PCR (qPCR) except primers and templates. The master mix contains a highly stable hot-start Taq polymerase in an optimized buffer with dsDNA specific SYBR green fluorescent dye. Consequently, the ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, ROX) features high stability during storage, even at 37°C for weeks. In addition to high sensitivity and signal intensity, the ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, ROX) performs a low background/ high specificity qPCR results, as well as a better compatibility with fast PCR program. With inert smart blue contrast dye, the ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, ROX) is ready-to-use and greatly reduces pipetting errors, while largely improving the reproducibility of the process. The ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, ROX) also includes ROX reference dye for normalizing the fluorescent reporter signal in real-time quantitative PCR. This master mix allows sensitive, precise amplification, real-time tracking of the amplification process, and simultaneous quantification for targeted DNA molecules.

Detail

Description 

The ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, ROX) is a ready-to-use reagent with all the essential components for quantitative real-time PCR (qPCR) except primers and templates. The master mix contains a highly stable hot-start Taq polymerase in an optimized buffer with dsDNA specific SYBR green fluorescent dye. Consequently, the ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, ROX) features high stability during storage, even at 37°C for weeks. In addition to high sensitivity and signal intensity, the ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, ROX) performs a low background/ high specificity qPCR results, as well as a better compatibility with fast PCR program. With inert smart blue contrast dye, the ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, ROX) is ready-to-use and greatly reduces pipetting errors, while largely improving the reproducibility of the process. The ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, ROX) also includes ROX reference dye for normalizing the fluorescent reporter signal in real-time quantitative PCR. This master mix allows sensitive, precise amplification, real-time tracking of the amplification process, and simultaneous quantification for targeted DNA molecules.

Features

  • High Stability
  • Fast Hot Start
  • High Sensitivity
  • Low Background / High Specificity 
  • Suitable for Fast Program 
  • Smart Blue Contrast Dye 
  • With ROX Reference Dye

Storage

Protect from light.
Aliquot to avoid multiple freeze-thaw cycles. 

-20°C for 12 months

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Place of Origin: Guangdong, China

Warranty: 2 years

Customized support: OEM

Brand Name: HKM

Model Number: CRM005

Reagent grade: Biochemical Reagent

Form: Powder

Color: Light yellow

Type: Bacillus Cereus Agar

D-3-Hydroxybutyric Acid (β-Hydroxybutyrate) Assay Kit

K-HDBA

SKU: 700004302

60 assays (manual) / 600 assays (microplate) / 740 assays (auto-analyser)

Content:60 assays (manual) / 600 assays (microplate) / 740 assays (auto-analyser)
Shipping Temperature:Ambient
Storage Temperature:Short term stability: 2-8oC,
Long term stability: See individual component labels
Stability:> 2 years under recommended storage conditions
Analyte:D-3-Hydroxybutyric Acid
Assay Format:Spectrophotometer, Microplate, Auto-analyser
Detection Method:Absorbance
Wavelength (nm):492
Signal Response:Increase
Linear Range:0.4 to 12 µg of D-3-hydroxybutyric acid per assay
Limit of Detection:0.074 mg/L
Reaction Time (min):~ 6 min
Application examples:Egg, egg products (e.g. egg powder) and other materials (e.g. biological cultures, samples, etc.).
Method recognition:Methods based on this principle have been accepted by EEC

The D-3-Hydroxybutyric Acid (β-Hydroxybutyrate) Assay Kit is suitable for the specific measurement and analysis of D-hydroxybutyric acid in eggs and egg products and other foods and beverages.

Note for Content: The number of manual tests per kit can be doubled if all volumes are halved.  This can be readily accommodated using the MegaQuantTM  Wave Spectrophotometer (D-MQWAVE).

Explore more organic acid assay kit products.

Scheme-K-HDBA HDBA Megazyme

Advantages

  • Very competitive price (cost per test) 
  • All reagents stable for > 2 years after preparation 
  • Very rapid reaction (~ 3 min) 
  • No wasted diaphorase solution (stable suspension supplied)  
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing 
  • Standard included 
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L-Malic Acid Assay Kit (Liquid Ready)

 K-LMALQR

SKU: 700004313

1100 assays (microplate) / 1100 assays (auto-analyser)

Content:1100 assays (microplate) / 1100 assays (auto-analyser)
Shipping Temperature:Ambient
Storage Temperature:Short term stability: 2-8oC,
Long term stability: See individual component labels
Stability:> 6 months under recommended storage conditions
Analyte:L-Malic Acid
Assay Format:Spectrophotometer, Auto-analyser
Detection Method:Absorbance
Wavelength (nm):340
Signal Response:Increase
Linear Range:0.5 to 18 μg of L-malic acid per assay
Limit of Detection:166 mg/L
Reaction Time (min):~ 3 min
Application examples:Wine, beer, fruit juices, soft drinks, candies, fruit and vegetables, bread, cosmetics, pharmaceuticals and other materials (e.g. biological cultures, samples, etc.).
Method recognition:Methods based on this principle have been accepted by AOAC, EEC, EN, NF, NEN, DIN, GOST, OIV, IFU, AIJN, NBN, ISO and MEBAK

This product has been discontinued, you may be interested in the following replacement product K-LMLQR; 700007622)

The L-Malic Acid (Liquid Ready) test kit is a rapid, simple, reliable and accurate method for the specific measurement and analysis of L-malic acid in wine, beverages, foodstuffs and other materials. Supplied as a “ready to use” liquid stable formulation that is suitable for auto-analyser and microplate formats.

Browse more test kit products in our organic acid assay kits list.

Scheme-K-LMALQR LMALQR Megazyme

Advantages

  • PVP incorporated to prevent tannin inhibition 
  • “Ready to use” liquid stable formulation 
  • Very competitive price (cost per test) 
  • All reagents stable for > 18 Months 
  • Very rapid reaction (~ 3 min) 
  • Standard included 
  • Suitable for microplate and auto-analyser formats