Introduction

The HiPure Midi system provides a fast, simple, and cost-effective plasmid DNA midiprep method for routine molecular biology laboratory applications. HiPure Midiprep Kits use silica membrane technology to eliminate the cumbersome steps associated with loose resins or slurries. Plasmid DNA purified with Mini Kits is immediately ready for use. Phenol extraction and ethanol precipitation are not required, and high quality plasmid DNA is eluted in a small volume of Tris buffer or water.

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Specifications

Principle

The HiPure Plasmid procedure is based on alkaline lysis of bacterial cells followed by adsorption of DNA onto silica in the presence of high salt. The unique silica membrane used in the kit completely replaces glass or silica slurries for plasmid DNA minipreps. The procedure consists of 3 basic steps: Preparation and clearing of a bacterial lysate by alkaline method，then transfer the supernatant to column to bind DNA. After washing proteins and other impurities, nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).

Advantages

• High purity – purified plasmid can be directly used in sequencing, enzyme digestion and PCR, etc.
• Fast – silica gel column purification is much faster than ion exchange
• High concentration – easily obtain concentrations >1000ng/μl
• Small amount large extraction – using a small amount of column to achieve a large extraction yield (500μg)
• Low endotoxin content – the obtained plasmid can be directly used in cell transfection and animal injection

Kit Contents

Storage and Stability

The kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. If any precipitates form in the buffers, warm at 37℃ to dissolve. After addition of RNase A and optional LyseBlue reagent, Buffer P1 is stable for 6 months when stored at 2–8°C.

Purchase Guide

For any technical problems or customized products, please contact us.

F&Q about Endotoxin-free Plasmid Extraction Kit — P1156 ←click here

The HiPure Midi system provides a fast, simple, and cost-effective plasmid DNA midiprep method for routine molecular biology laboratory applications. HiPure Midiprep Kits use silica membrane technology to eliminate the cumbersome steps associated with loose resins or slurries. Plasmid DNA purified with Mini Kits is immediately ready for use. Phenol extraction and ethanol precipitation are not required, and high quality plasmid DNA is eluted in a small volume of Tris buffer or water.

Overview

• Complete, user-friendly system to collect, preserve and transport stool samples
• Each clamshell contains nitrile gloves, alcohol swabs, Fe-Col® Collection Paper, and Norgen’s Stool Nucleic Acid Collection and Preservation Tube containing Norgen’s Stool Preservative
• The preservative provides sample homogeneity eliminating sample variability
• Preserve and transport DNA & RNA safely at ambient temperature
• Nucleic acid preservation at room temperature over 2 years for DNA and 7 days for RNA
• No cold shipping/storage needed – hassle-free and cost effective
• Isolate nucleic acids for any application including 16s NGS
• Robust preservation over a range of temperatures
• Customizable with various accessories for easy and safe collection
• Eliminates odor and renders samples safe and non-infectious

Norgen’s Stool Nucleic Acid Collection and Preservation System is a complete system designed for collection, ambient storage and transport of nucleic acids from stool specimens. Each of the 50 clamshells contain: nitrile gloves, alcohol swabs, Fe-Col® Collection Paper, and Norgen’s Stool Nucleic Acid Collection and Preservation Tube containing Norgen’s Stool Preservative in a liquid format. The user simply collects the stool specimen using the provided Fe-Col® Collection Paper, and then transfers the stool into the Stool Nucleic Acid Collection and Preservation Tube using the spoon attached to the lid. The sample is then mixed gently until the stool is well submerged under the preservative. The Stool Preservative eliminates the need to immediately process or freeze samples and allows the samples to be shipped to centralized testing facilities at ambient temperatures. The components of the Stool Preservative allow samples to be stored at room temperature for over 2 years for DNA and up to 7 days for RNA. To extend the stool RNA stability in the preservative, storage at -20°C or -70°C is recommended upon arrival at the testing facilities until RNA purification. The Stool Preservative also prevents the growth of Gram-negative and Gram-positive bacteria and fungi, and inactivates viruses allowing the resulting non-infectious samples to be handled and shipped safely.

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Extracting Biological Insights from Stool

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Details

Supporting Data

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* The RNA stability will vary depending on the samples

Storage Conditions and Product Stability

All tubes should be kept tightly sealed and stored at room temperature (15 – 25°C) for up to 2 years without any reduction in performance.

Propargyl-PEG5-CH2CO2tBu enables the formation of triazole linkages with azide compounds or biomolecules in copper catalyzed Click Chemistry reactions. The t-butyl group can be hydrolyzed under acidic conditions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Propargyl-PEG5-CH2CO2tBu enables the formation of triazole linkages with azide compounds or biomolecules in copper catalyzed Click Chemistry reactions. The t-butyl group can be hydrolyzed under acidic conditions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.