
Tri(propargyl-PEG2-NHCO-ethyloxyethyl)amine Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Tri(propargyl-PEG2-NHCO-ethyloxyethyl)amine Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Tri(propargyl-PEG2-NHCO-ethyloxyethyl)amine Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
K-MRGLUC
1000 assays (microplate) / 100 assays (manual) / 400 assays (auto-analyser)
Content: | 1000 assays (microplate) / 100 assays (manual) / 400 assays (auto-analyser) |
Shipping Temperature: | Ambient |
Storage Temperature: | Short term stability: 2-8oC, Long term stability: See individual component labels |
Stability: | > 2 years under recommended storage conditions |
Analyte: | D-Glucose |
Assay Format: | Spectrophotometer, Microplate, Auto-analyser |
Detection Method: | Absorbance, Fluorescence |
Wavelength (nm): | (Fluorescence) 530-560 (ex), ~ 590 (em) (Absorbance) 570 |
Signal Response: | Increase |
Linear Range: | (Fluorescence) 0.009-0.18 μg of D-glucose per assay (0.5-10 μM in-assay) (Absorbance) 0.9-9.0 μg of D-glucose per assay (5-50 μM in-assay) |
Limit of Detection: | (Fluorescence) 0.09 ug/mL (Absorbance) 0.25 ug/mL |
Reproducibility (%): | (Fluorescence) ~ 6% (Absorbance) ~ 7% |
Reaction Time (min): | (Fluorescence) 30 min (Absorbance) 30 min |
Application examples: | Food and beverage samples such as wine, beer, fruit juices, soft drinks, milk, jam, dietetic foods, bakery products, candies, fruit and vegetables. Other samples such as tobacco, cosmetics, pharmaceuticals (e.g. infusions), feed, paper (and cardboard) and other materials (e.g. biological cultures, samples, etc.). |
This product has been discontinued.
The D-Glucose Assay Kit (Megaplex Red) provides a simple robust method for the measurement of D-glucose. This kit utilises a highly sensitive Megaplex Red probe which when coupled to horseradish peroxidase (HRP) and glucose oxidase (GOX) enzymatic reactions, allows for the measurement of D-glucose in a sample. Upon oxidation of the probe by HRP, a coloured product (resorufin) is formed that can be measured in fluorescence mode using excitation between 530-560 nm and emission at ~ 590 nm* or colourimetrically at 570 nm.
*The excitation and emission maxima of Megaplex Red are 570 nm and 585 nm respectively.
See more related mono/disaccharide assay kit products.
Fluorometric/UV method for the determination of D-glucose content in a variety of samples.
Advantages
The D-Glucose Assay Kit (Megaplex Red) provides a simple robust method for the measurement of D-glucose. This kit utilises a highly sensitive Megaplex Red probe which when coupled to horseradish peroxidase (HRP) and glucose oxidase (GOX) enzymatic reactions, allows for the measurement of D-glucose in a sample. Upon oxidation of the probe by HRP, a coloured product (resorufin) is formed that can be measured in fluorescence mode using excitation between 530-560 nm and emission at ~ 590 nm* or colourimetrically at 570 nm.
Norgen’s FFPE RNA Purification Kits provide a rapid method for the isolation and purification of total RNA (including microRNA) from formalin-fixed paraffin-embedded (FFPE) tissue samples in as little as 1 hour. Using formalin to fix tissues leads to crosslinking of the RNA and proteins, and the process of embedding the tissue samples can also lead to fragmentation of the RNA over time. Norgen’s FFPE RNA Purification Kits provide conditions that allow for the partial reversing of the formalin modifications, resulting in a high quality and yield of RNA. These kits are able to purify all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA), depending on the age of the FFPE tissue as fragmentation of the RNA is known to occur over time. The RNA is preferentially purified from other cellular components without the use of phenol or chloroform.
FFPE RNA Purification Kit (Spin Column)
Maximum loading volume of 650 μL per column, and a maximum binding capacity of 50 μg per column.
FFPE RNA Purification 96-Well Kit (High Throughput)
Purification is based on 96-well column chromatography using Norgen’s proprietary resin as the separation matrix. Purification can be performed using either a vacuum manifold or centrifugation. Maximum loading volume of 400 μL per well, and a maximum binding capacity of 50 μg per well.
Figure 1 / 5
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Kit Specifications | |
Maximum Column Binding Capacity | Up to 50 µg RNA |
Maximum Loading Volume Per Spin Column | 650 µL |
Size of RNA Purified | All sizes, including small RNA (< 200 nt) |
Time to Complete 10 Purifications | 1-4 hours* |
Maximum Amount of Starting Material | 5 slices of < 20 µm thick paraffin slices 25 mg of unsectioned block |
Average Yield | Variable due to age of paraffin blocks ~2-3 µg of Total RNA per 1 mg of fresh FFPE hamster kidney |
* Time required for purification varies by length of Proteinase K incubation and formalin crosslink-reversal
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. The DNAse I and Proteinase K should be stored at -20°C upon arrival. This kit is stable for 1 year from the date of shipment.
Component | Cat. 25300 (50 preps) | Cat. 25400 (2 x 96 preps) |
---|---|---|
Digestion Buffer A | 25 mL | 2 x 25 mL |
Buffer RL | 30 mL | 2 x 30 mL |
Enzyme Incubation Buffer | 6 mL | 2 x 6 mL |
Wash Solution A | 38 mL | 2 x 38 mL |
Elution Solution A | 6 mL | 2 x 20 mL |
Proteinase K | 12 mg | 2 x 20 mg |
DNase I | 1 vial | 2 x 500μL |
Micro Spin Columns | 50 | – |
96-Well Incubation Plate | – | 2 |
96-Well Plate | – | 2 |
Adhesive Tape | – | 8 |
Collection Tubes | 50 | – |
96-Well Collection Plate | – | 2 |
Elution Tubes (1.7 mL) | 50 | – |
96-Well Elution Plate | – | 2 |
Product Insert | 1 | 1 |
Propargyl-PEG2-amine is a crosslinker that is reactive with NHS esters, carbonyls (carboxylic acid, ketone, aldehyde). The propargyl group can participate in copper catalyzed azide-alkyne Click Chemistry reaction to form a stable triazole linkage. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Propargyl-PEG2-amine is a crosslinker that is reactive with NHS esters, carbonyls (carboxylic acid, ketone, aldehyde). The propargyl group can participate in copper catalyzed azide-alkyne Click Chemistry reaction to form a stable triazole linkage. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
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