Tri(propargyl-PEG2-NHCO-ethyloxyethyl)amine Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Tri(propargyl-PEG2-NHCO-ethyloxyethyl)amine Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Tri(propargyl-PEG2-NHCO-ethyloxyethyl)amine Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
HiPure Bacterial RNA Kit uses silica gel column purification to simplify the extraction. The whole process does not require phenol chloroform extraction and time-consuming alcohol precipitation. The kit is suitable for efficiently extracting RNA from various bacterial samples. The purified RNA can be directly used for RT-PCR, Northern hybridization, etc. The kit has included lysozyme and glass beads, which can be used to treat gram-negative bacteria which is easy to be lysed, as well as gram-positive bacteria which is hard to be lysed, including enterococcus faecalis, staphylococcus, etc.
Specifications
| Features | Specifications |
| Main Functions | Isolation total RNA from bacteria culture |
| Applications | RT-PCR, Northern blot, poly-A + purification, nucleic acid protection and in vitro translation |
| Purification method | Mini spin column |
| Purification technology | Silica technology |
| Process method | Manual (centrifugation or vacuum) |
| Sample type | Bacteria culture |
| Sample amount | Bacteria: <10^9 |
| Elution volume | ≥30μl |
| Time per run | ≤40 minutes |
| Liquid carrying volume per column | 800µl |
| Binding yield of column | 100µg |
Hipure silica gel column is based on glass fiber filter membrane with high binding force. Under the condition of high concentration of ionizing agent (such as guanidine hydrochloride or guanidine isothiocyanate), the filter membrane can adsorb nucleic acid through hydrogen bond and electrostatic, while protein and other impurities are not adsorbed and removed. The filter membrane adsorbed with nucleic acid is washed to remove the residual protein and salt. Finally, the nucleic acid adsorbed on the filter membrane can be washed out with low salt buffer (such as buffer TE) or water. The obtained nucleic acid has high purity and can be directly used in various downstream experiments.
Advantages
Kit Contents
| Contents | R418101 | R418102 | R418103 |
| Purification Times | 10 Preps | 50 Preps | 250 Preps |
| gDNA Filter Mini Columns | 10 | 50 | 250 |
| HiPure RNA Mini Columns | 10 | 50 | 250 |
| 2ml Collection Tubes | 20 | 100 | 500 |
| Glass Beads (0.1-0.6mm) | 10 g | 30 g | 150 g |
| Plastic spoon | 2 | 4 | 10 |
| Lysozyme | 20 mg | 90 mg | 400 mg |
| Protease Dissolve Buffer | 1.8 ml | 1.8 ml | 10 ml |
| Buffer TE | 1.8 ml | 1.8 ml | 5 ml |
| Buffer STL | 5 ml | 20 ml | 90 ml |
| Buffer RLC | 10 ml | 30 ml | 150 ml |
| Buffer RW1 | 10 ml | 50 ml | 250 ml |
| Buffer RW2* | 5 ml | 20 ml | 2 x 50 ml |
| RNase Free Water | 1.8 ml | 10 ml | 30 ml |
Storage and Stability
The kit components can be stored at room temperature (15–25°C) and are stable for at least 18 months under these conditions. Due to the lack of antibacterial agents, RNase Free Water may be contaminated by bacterial or fungal when placed or operated at room temperature. It is recommended to pack and store at 2-8°C to reduce contamination.
HiPure Bacterial RNA Kit uses silica gel column purification to simplify the extraction. The whole process does not require phenol chloroform extraction and time-consuming alcohol precipitation. The kit is suitable for efficiently extracting RNA from various bacterial samples. The purified RNA can be directly used for RT-PCR, Northern hybridization, etc. The kit has included lysozyme and glass beads, which can be used to treat gram-negative bacteria which is easy to be lysed, as well as gram-positive bacteria which is hard to be lysed, including enterococcus faecalis, staphylococcus, etc.
Figure 1 / 1
Click for expanded view
Ladder Properties:
• 22 discrete bands, ranging from 60 bp to 600 bp
Storage:
• Store at -20°C in small aliquots. For longer term storage, -70°C is recommended.
• The product is light-sensitive. Minimize light exposure.
Norgen’s Total RNA Purification Kits Dx provide rapid methods for the isolation and purification of total RNA from tissue samples, blood, plasma, serum, saliva, bacteria, yeast, fungi and viruses for subsequent in vitro diagnostic use. Extract high quality and purity RNA with excellent RIN values and A260/A280 suitable for downstream applications including qRT-PCR, RT-PCR, microarrays, NGS and more.
Total RNA Purification Kit Dx (Spin Column)
This kit is designed to be used with any downstream application employing enzymatic amplification or other enzymatic modifications of RNA followed by signal detection or amplification. Any diagnostic results generated using the RNA isolated with Norgen’s Total RNA Purification Kit Dx in conjunction with an in vitro diagnostic assay should be interpreted with regard to other clinical or laboratory findings.
Total RNA Purification 96-Well Kits Dx (High Throughput and High Throughput Deep Well)
Thess 96-well kits provide a rapid method for the high-throughput isolation and purification of total RNA in 30 minutes using vacuum manifold, plate centrifuge, or liquid handlers with vacuum capabilities. Total RNA can be isolated from a broad range of sample sources including cultured cells, tissues, blood, serum, plasma, bacteria, yeast, fungi, and viruses.
Intended Use
To minimize irregularities in diagnostic results, suitable controls for downstream applications should be used. Norgen’s Total RNA Purification Kits Dx are intended for use by professional users such as technicians, physicians and biologists experienced and trained in molecular biological techniques. Norgen’s Total RNA Purification Kits Dx do not provide a diagnostic result. It is the sole responsibility of the user to use and validate the kit in conjunction with a downstream in vitro diagnostic assay.
NOTE: This product is not available for sale in the United States.
Figure 1 / 13
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| Kit Specifications | |
| Maximum Binding Capacity Per Well | 50 μg |
| Maximum Loading Volume Per Well | 500 μL |
| Size of RNA Purified | All sizes, including small RNA < 200 nt |
| Time to Complete 96 Purifications | 30 minutes |
| Average Yield HeLa Cells (1 x 106 Cells) E. coli (1 x 109 cells) Brain (10 mg) Liver (10 mg) Blood (50 μL, Hamster) | 15 μg 50 μg 9 μg 26 μg 1 μg |
| Maximum Amount of Starting Material: Whole Blood Plasma/Serum Bacteria Yeast Fungi Plant Tissue | 100 µL150 µL1 x 109 cells1 x 108 cells 40 mg 40 mg |
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers.
| Component | Cat. Dx17200 (50 preps) | Cat. Dx24300 (192 preps) | Cat. Dx24350 (192 preps) | Cat. Dx24380 (576 preps) |
|---|---|---|---|---|
| Lysis Solution | 40 mL | – | – | – |
| Buffer RL | – | 2 x 40 mL | 2 x 40 mL | 350 mL |
| Wash Solution | 22 mL | – | – | – |
| Wash Solution A | 22 mL | 2 x 38 mL | 2 x 38 mL | 1 x 148 mL 1 x 74 mL |
| Elution Solution | 6 mL | – | – | – |
| Elution Solution A | – | 2 x 20 mL | 2 x 20 mL | 60 mL |
| Mini Spin Columns | 50 | – | – | – |
| 96-Well Isolation Plate | – | 2 | 2 | – |
| 96-Well Isolation Plate (Deep Well) | – | – | – | 6 |
| Collection Tubes | 50 | – | – | – |
| 96-Well Collection Plate | – | 2 | 2 | – |
| 96-Well Collection Plate (Deep Well) | – | – | – | 6 |
| Elution Tubes (1.7 mL) | 50 | – | – | – |
| 96-Well Elution Plate | – | 2 | 2 | – |
| 96-Well Elution Plate (Deep Well) | – | – | – | 6 |
| Adhesive Tape | – | 4 | 4 | 12 |
| Product Insert | 1 | 1 | 1 | 1 |