Tube sealer

The blood bag tube sealer is a compact equipment to seal the blood bags, infusion bags, urine bags by high frequency sealing system. It is widely used in blood bank, hospital, biological industry, etc.

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Application

The blood bag tube sealer is a compact equipment to seal the blood bags, infusion bags, urine bags by high frequency sealing system. It is widely used in blood bank, hospital, biological industry, etc.

Features

No Hemolysis

With suction feet to avoid moving, can be used in blood collecting vehicle.

Technical Parameters

Model	HG-BTS1
Sealing time	2-4S
Sealing Type	Automatic
Diameter of tube	φ2-6mm(Tube thickness≤1.5mm)
Powe supply	220V 0.7A
Consumption	150W
External Size	310190150mm
Package Size	405290240mm
Net/Gross Weight	6/7kgs

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AZtaq™ DNA Polymerase

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Plasma/Serum RNA Purification Kits

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Overview

Isolate all sizes of circulating and exosomal RNA, including microRNA
Versatile plasma/serum input ranges
No phenol extractions
No carrier RNA
Bind and elute all RNA irrespective of size or GC content, without bias
Concentrate circulating RNA and exosomal RNA into a flexible elution volume
High quality, purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen’s NGS services
Compatible with Streck Cell-Free RNA BCT® Tubes
Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

These kits provide a fast, reliable and convenient method to purify and concentrate high quality, high purity and inhibitor-free cell-free circulating and exosomal RNA using a convenient spin column method. These kits can purify RNA from fresh or frozen serum or plasma samples prepared from blood collected on either EDTA or Citrate. Plasma samples prepared from blood collected on heparin should not be used, as heparin can significantly interfere with many downstream applications such as RT-PCR. The purified plasma/serum RNA is fully compatible with all downstream applications including PCR, qPCR, methylation-sensitive reverse transcription qPCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, expression array assays, and NGS.

Background

Plasma/Serum cell-free circulating RNA or exosomal RNA has the potential to provide biomarkers for certain cancers and disease states. Exosomes are 40 – 150 nm membrane vesicles, which are secreted by most cell types. Exosomes can be found in saliva, blood, urine, amniotic fluid and malignant ascitic fluids, among other biological fluids. Evidence has been accumulating recently that these vesicles act as cellular messengers, conveying information to distant cells and tissues within the body. These exosomes may play a functional role in mediating adaptive immune responses to infectious agents and tumours, tissue repair, neural communication and transfer of pathogenic proteins. For this reason exosomal RNAs may serve as biomarkers for various diseases including cancer. As the RNA molecules encapsulated within exosomes are protected from degradation by RNAses they can be efficiently recovered from biological fluids, such as plasma or serum.

Plasma/Serum RNA Purification Mini Kit

This kit can purify RNA from fresh or frozen serum or plasma samples prepared from blood collected on either EDTA or Citrate, from volumes ranging from 50 µL to 200 µL. The purified plasma/serum RNA is eluted in a flexible final volume of 10 µL to 25 µL.

Plasma/Serum RNA Purification Midi Kit

This utilizes a two column method, and can purify RNA from fresh or frozen serum or plasma samples prepared from blood collected on either EDTA or Citrate, from volumes ranging from 250 µL to 1.5 mL. The first column will handle the large volume input of bodily fluids that is followed by a concentration on a mini column for a final elution of 50 µL to 100 µL.

Plasma/Serum RNA Purification Maxi Kit

This kit can purify RNA from fresh or frozen serum or plasma samples prepared from blood collected on either EDTA or Citrate, from volumes ranging from 2 mL to 5 mL. The first column will handle the large volume input of bodily fluids that is followed by a concentration on a mini column for a final elution of 50 µL to 100 µL.

Isolate RNA after Purifying EVs and Exosomes

For Ultracentrifugation, Exoquick, and Filtration

Cat. #	Name	Elution Volume	Plasma/Serum	Urine	Cell-Culture Media
55000	Plasma/Serum RNA Purification Mini Kit	10 – 25 µL	50 µL – 1 mL	250 µL – 1 mL	5 – 10 mL
35300	Total RNA Purification Micro Kit	20 – 50 µL	1 – 4 mL	2 – 10 mL	10 – 20 mL
17200	Total RNA Purification Kit	50 – 100 µL	4 – 10 mL	11 – 30 mL	20 – 35 mL

Details

Supporting Data

Figure 1 / 13

Figure 1. Purification of circulating RNA from different plasma volumes.

Figure 2. Eluting purified circulating RNA from into different elution volumes.

Figure 3. Effective and consistent detection of miRNA from plasma.

Figure 4. Small RNA Sequencing from as little as 50 μL of Plasma.

Figure 5. High Degree of Overlapping of miRNA.

Figure 6. Increased Diversity of Other Small RNA Species.

Figure 7. Better Diversity of miRNA Detected from HeLa Cells.

Figure 8. Purification of cell-free circulating RNA and exosomal RNA from different plasma volumes.

Figure 9. Linearity of RNA purified from increasing plasma volumes using Norgen’s Plasma/Serum RNA Purification Midi Kit.

Figure 10. Determination of the amount of inhibition present in plasma RNA samples when detecting the human 5S transcript and miR-21.

Figure 11. Purification of cell-free circulating RNA and exosomal RNA from different plasma volumes.

Figure 12. Linearity of RNA purified from increasing plasma volumes using Norgen’s Plasma/Serum RNA Purification Maxi Kit.

Figure 13. Determination of the amount of inhibition present in plasma RNA samples when detecting the human 5S transcript and miR-21.

Kit Specifications
Minimum Plasma/Serum Input	2 mL
Maximum Plasma/Serum Input	5 mL
Size of DNA Purified	All sizes, including miRNA and small RNA (<200 nt)
Elution Volume	50-100 μL
Time to Complete 10 Purifications	35-40 minutes
Average Yields*	Variable depending on specimen

*Please check page 7 of the Product Insert for Average Plasma/Serum Yields and Common RNA Quantification Methods.

This kit is suitable for the isolation of RNA from fresh or frozen serum or plasma prepared from blood collected on either EDTA or Citrate. Plasma samples prepared from blood collected on heparin should not be used as heparin can significantly interfere with many downstream applications such as RT-PCR.

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.

Kit Components	Cat. 55000 (50 preps)	Cat. 56100 (20 preps)	Cat. 56200 (10 preps)
Lysis Buffer A	2 x 20 mL	100 mL	1 x 130 mL 1 x 30 mL
Wash Solution A	18 mL	1 x 38 mL 1 x 18 mL	38 mL
Elution Solution A	6 mL	6 mL	6 mL
Elution Buffer F	–	15 mL	15 mL
Micro Spin Columns	50	–	–
Mini Spin Columns	–	20	10
Midi Spin Columns	–	20	–
Maxi Spin Columns	–	–	10
Collection Tubes	50	20	10
Elution Tubes (1.7 mL)	50	20	10
Product Insert	1	1	1

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α-Glucuronidase Assay Kit

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Content:	50 assays (manual) / 200 assays (microplate)
Shipping Temperature:	Ambient
Storage Temperature:	Short term stability: 2-8^oC, Long term stability: See individual component labels
Stability:	> 2 years under recommended storage conditions

Analyte:	α-Glucuronidase
Assay Format:	Spectrophotometer, Microplate
Detection Method:	Absorbance
Wavelength (nm):	340
Signal Response:	Increase
Linear Range:	17.4 to 174 mU/mL of α-D-glucuronidase per assay
Limit of Detection:	17 mU/mL
Reaction Time (min):	~ 25 min
Application examples:	Enzyme preparations and other materials.
Method recognition:	*Novel method*

Tube sealer

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AZtaq™ DNA Polymerase

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Key Features

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Properties

Plasma/Serum RNA Purification Kits

Overview

Details

Supporting Data

α-Glucuronidase Assay Kit

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