Real-time fluorescent DNA amplification made possible with a TwistAmp® exo kit. Recommended for users who want to combine TwistDx’s RPA amplification technology with the use of TwistDx’s proprietary fluorescent TwistAmp® exo probe in a homogenous format. The user need only supply primers, probe, and template. See manual for more information. Click to order oligonucleotides.
Real-time fluorescent DNA amplification made possible with a TwistAmp® exo kit. Recommended for users who want to combine TwistDx’s RPA amplification technology with the use of TwistDx’s proprietary fluorescent TwistAmp® exo probe in a homogenous format. The user need only supply primers, probe, and template. See manual for more information. Click to order oligonucleotides.
Adenoviral vectors are useful tools for both in vitro and in vivo gene transfer, and oncolytic viruses based on adenovirus are highly promising for cancer treatment. Norgen’s Adenovirus Purification Kit provides a fast and simple procedure for concentrating and purifying adenoviral vectors from cell lysate and cell culture media. Purification is based on spin column chromatography using Norgen’s proprietary resin as an ion exchanger. Contaminating cellular debris is largely removed from the sample via a centrifugation step, while contaminating DNA and RNA is reduced using enzymatic digestion. Adenoviral vectors purified in this manner are highly active for use in transduction experiments.
Norgen’s Adenovirus Purification Kit contains sufficient materials for 15 preparations (33.5 mL per prep of supernatant or a total of 500 mL of supernatant input). Approximately 1 mL of cell pellet can be purified per prep, up to a maximum of 15 mL of cell pellet in total for the entire kit.
Figure 1 / 3
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| Kit Specifications | |
| Resin Binding Capacity (total per kit) | At least 3×1011 adenovirus particles as determined by qPCR At least 3×108 transducing units as determined by transduction assay |
| Input type | Cells, media |
| Input volume (Supernatant) | 1 – 33.5 mL SN per prep (500 mL SN in total) |
| Input Volume (Cell pellet) | 1 mL cell pellet per prep (15 mL in total) |
| Minimum elution volume | 1.3 mL per prep |
| Time to complete purification | 2.5 to 4.5 hours with 1 hour hands on time |
| In vivo transduction | Yes |
Storage Conditions and Product Stability
HL-SAN Nuclease should be stored at -20°C upon arrival. Elution Buffer P should be stored tightly capped at 4°C upon arrival. All other solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
| Component | Cat. 67600 (15 preps) |
|---|---|
| Lysis Buffer S | 5.5 mL |
| HL-SAN Nuclease | 102 µL |
| Binding Buffer A | 20 mL |
| Purification Solution C | 60 mL |
| Purification Solution D | 130 mL |
| Wash Solution C | 2 x 130 mL |
| Slurry E | 12.5 mL |
| Elution Buffer P (store at 4°C) | 66 mL |
| Protein Neutralizer | 4 mL |
| Elution tubes (1.7 mL) | 50 |
| Product Insert | 1 |
The NGS Library Circularization Kit (MGI Platform) was developed for preparation of single-stranded circular DNA libraries for next generation sequencing (MGI platform).
The kit uses linear dsDNA libraries (MGI platform) as input and enriches the circularized single-stranded DNA libraries. The circularization kit has a higher library circularization efficiency (25%) as compared to other vendors (7-15%).
NGS Library Circularization Kit workflow
Comparison of Library Circularization Efficiency
Kit features
The NGS Library Circularization Kit (MGI Platform) was developed for preparation of single-stranded circular DNA libraries for next generation sequencing (MGI platform).
TDL5E Bench top low speed refrigerated centrifuge machine
Application:
Widely used in the field of biochemistry, biological products, pharmaceutical factory and laboratory.
TDL5E Technical Parameters:
| Max. Speed | 5000rpm | Max.RCF | 4420xg |
| Max. Volume | 4x180ml | Speed Accuracy | ±20rpm |
| Timer | 1~99h59min | Noise | ≤60dBA |
| Dimension(LxWxHmm) | 570x600x340mm | Net Weight | 76Kg |
| Power Supply | AC220V/110V 50HZ/60HZ | Temperature Range | -20℃~40℃ |
| Temperature Accuracy | ±1℃ | Certifications | CE, ISO & Calibration report is available. |
Matched Rotors for TDL5E:
| Order No. | Rotor Type | Max. Speed(rpm) | Volume(ml) | Max.RCF(xg) |
| 5E-1 | Swing rotor | 4000 | 4x180ml | 2580 |
| 5E-2 | Microplate rotor | 4000 | 2x3x96well | 2020 |
| 5E-3 | Swing rotor | 5000 | 4x1x50ml | 4420 |
| 5E-4 | Swing rotor | 5000 | 4x1x100ml | 4420 |
| 5E-5 | Swing rotor | 4000 | 4x2x50ml | 2820 |
| 5E-6 | Swing rotor | 4000 | 4x4x15ml | 2820 |
| 5E-7 | Swing rotor | 4000 | 4x6x10/7ml | 2580 |
| 5E-8 | Swing rotor | 4000 | 4x6x5ml | 2170 |
| 5E-9 | Swing rotor | 4000 | 4x4x10/7ml | 2580 |
| 5E-10 | Swing rotor | 4000 | 4x4x5ml | 2170 |
| 5E-11 | Fixed rotor | 5000 | 6x15ml | 2540 |
| 5E-12 | Fixed rotor | 5000 | 12x15ml | 3080 |
| 5E-13 | Fixed rotor | 5000 | 24x15ml | 3500 |
| 5E-14 | Fixed rotor | 5000 | 4x50ml | 2520 |
| 5E-15 | Fixed rotor | 5000 | 6x50ml | 2850 |
| 5E-16 | Fixed rotor | 5000 | 4x100ml | 2630 |
| 5E-17 | Fixed rotor | 5000 | 6x100ml | 3130 |
TDL5E is a table top low speed refrigerated centrifuge widely used in the field of biochemistry, biological products, pharmaceutical factory and laboratory.
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Tel : 081-875-1869 , 02-328-7179
Email : [email protected]
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