Real-time fluorescent DNA amplification in a flexible liquid format. Recommended for users who want to combine RPA with the use of TwistDx’s proprietary fluorescent TwistAmp® exo Probes. In addition to the basic components, a powerful nuclease (Exonuclease III) is provided which will process TwistAmp® exo Probes during the amplification reaction itself and generate a real-time readout. The user need only supply primers, probe, dNTPs and template.See manual for more information. Click to order oligonucleotides.
Detail
Real-time fluorescent DNA amplification in a flexible liquid format. Recommended for users who want to combine RPA with the use of TwistDx’s proprietary fluorescent TwistAmp® exo Probes. In addition to the basic components, a powerful nuclease (Exonuclease III) is provided which will process TwistAmp® exo Probes during the amplification reaction itself and generate a real-time readout. The user need only supply primers, probe, dNTPs and template.See manual for more information. Click to order oligonucleotides.
Perfect for: Real-time fluorescent DNA detection, facilitating use of different RPA reaction volumes, or variation of component ratios.
Product code: TALQEXO01
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CRM002 Chromogenic E.coli Agar
Product Info
Document
Product Info
Introduction
Usages:
For the rapid detection and enumeration of Escherichia coli.
Principle:
Peptone and yeast extract powder provides carbon and nitrogen sources and trace elements; sodium chloride maintains osmotic equilibrium; agar as medium coagulant; dodecyl sulfate inhibit Gram-positive bacteria; chromogenic substrate and large intestine coli β- glucuronidase enzyme specific reaction, hydrolysis of the substrate, the release of the color groups produce green colonies on the light yellow plate.
Formulation (per liter):
Peptone :15.0g
Yeast extract powder: 3.0g
Sodium chloride: 5.0g
Sodium lauryl sulfate:0.1g
Agar: 12.0g
Chromogenic substrate 6.5g
Final pH 7.0 ± 0.2
How to use:
1. Weigh 41.6g of the product, adding 1.0L distilled or deionized water, heated to boiling stirring until completely dissolved, dispensing into flask, 115 autoclaved for 10minutes.
2. Take 25.0g or 25.0ml of sample with sterile procedures , added to the flask containing 225.0mL of sterile phosphate buffered saline (or saline) , shaken thoroughly homogenized with a homogenizer or a 1:10 dilution of 1min solution, diluted 1:10 and then continue to select the appropriate serial dilutions of three, the two plates inoculated with each dilution, poured dissolved by heating and cooled to about 45 medium.
3, observe the results.
Quality Control
This product appears light yellow after the pouring on plate, these strains were inoculated after 36 ± 1 18 ~ 24h culture growth in the following table.
Bacteria name Bacteria NO. Growth Situation Feature
Escherichia coli ATCC25922 good green colonies
Citrobacter ATCC8090 good colorless colonies
Salmonella typhimurium CMCC50115 good colorless colonies
Enterococcus faecalis ATCC29212 suppressed —–
Storage: Store in a dark, cool and dry place, tighten the caps immediately after use. Storage period of two years.
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