Permagen’s 15 mL/ 50 mL Centrifuge Tube Magnetic Combo rack is designed for magnetic bead separations from up to three each, 15 mL or 50 mL Centrifuge tubes
Accommodates any common 15 mL Centrifuge Tubes and or 50 mL Centrifuge Tubes
Beads will be pulled to back wall allowing easy aspiration and tip tracking down the front wall of the tubes without disturbing bead pellet
Features include solid aluminum alloy design with hard coat finish for years of trouble-free use, rubber feet to help prevent slipping on work bench, less tippy than common plastic products, and fast separations using any magnetic beads
MSR1550
Maximum Volume – 50 mL (50 mL Tube)
Minimum Volume – 7 mL (15 mL Tube)
Permagen’s 15 mL/ 50 mL Centrifuge Tube Magnetic Combo rack is designed for magnetic bead separations from up to three each, 15 mL or 50 mL Centrifuge tubes
Epstein-Barr Virus (EBV) is a member of the Herpes family of virus and is one of the most common viruses in humans. The virus occurs globally and causes infectious mononucleosis. Most individuals become infected with EBV and develop adaptive immunity, with the majority of adults between the ages of 35 and 40 having been infected. The virus has been implicated as having a primary role in multiple autoimmune diseases, several lymphoproliferative disorders and cancers, particularly Hodgkin’s disease and Burkitt’s lymphoma. Many children become infected with EBV once maternal antibody protection disappears, however these infections usually do not result in symptom development. During adolescence the virus will cause mononucleosis in up to 69% of infections
EBV TaqMan PCR Kit, 100 reactions
EBV TaqMan PCR Probe/Primer Set and Controls, 100 reactions
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Storage Conditions and Product Stability
All kit components can be stored for 2 years after the date of production without showing any reduction in performance.
All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.
Component | Cat. TM41050 (100 preps) | Cat. TM41010 (100 preps) |
---|---|---|
MDx TaqMan 2X PCR Master Mix | 2 x 700 μL | – |
EBV Primer & Probe Mix | 280 μL | 280 μL |
EBV Positive Control | 150 μL | 150 μL |
Nuclease-Free Water (Negative Control) | 1.25 mL | 1.25 mL |
Product Insert | 1 | 1 |
This product is suitable for rapid extraction of RNA (include miRNA) from tissue, cells, blood, s and other clinical samples. RNA can be used directly for RT-PCR, quantitative RT-PCR, test of virus DNA and so on.
Specifications
Features | Specifications |
Main Functions | Isolation total RNA(miRNA)from tissue, cell using two columns and DNase plus reagent |
Applications | RT-PCR, cDNA synthesis, second generation sequencing |
Products | RNA, miRNA |
Purification method | Mini spin column |
Purification technology | Silica technology |
Process method | Manual (centrifugation or vacuum) |
Sample type | Clinical tissues, cells, lymphocytes |
Sample amount | Tissue: <20 mgCells: <5 x 106 |
Yield | 2-50μg |
Elution volume | ≥30μl |
Time per run | ≤25 minutes |
This product is based on silica column purification. Remove paraffin by Buffer DPS. Sample lysis withproteinase K digestion requires only 15 minutes. After lysis, samples are incubated at 80ºC for 15 minutes. Transfer to an adsorption column and RNA is adsorbed on the membrane, while protein is not adsorbed andis removed with filtration. After washing proteins and other impurities, RNA was finally eluted with low-saltbuffer.
Advantages
Kit Contents
Contents | IVD4121 |
Purification Times | 50 Preps |
HiPure DNA Mini Column Ⅱ | 50 |
HiPure RNA Mini Columns | 50 |
2ml Collection Tubes | 150 |
Proteinase K | 24 mg |
Protease Dissolve Buffer | 1.8 ml |
DNase I | 600 μl |
DNase Buffer | 6 ml |
Buffer RTL | 40 ml |
RNA Digestion Buffer | 15 ml |
Buffer RWC* | 20 ml |
Buffer RW2* | 20 ml |
Nuclease Free Water | 10 ml |
Storage and Stability
Proteinase K should be stored at 2–8°C upon arrival. DNase I should be stored at -20°C. However, short-term storage (DNase I up to 1 weeks, Proteinase K up to 8 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for at least 18 months under these conditions.
This product is suitable for rapid extraction of RNA (include miRNA) from tissue, cells, blood, s and other clinical samples. RNA can be used directly for RT-PCR, quantitative RT-PCR, test of virus DNA and so on.
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Tel : 081-875-1869 , 02-328-7179
Email : hej@a3p-scientific.com
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