Ultrapure Nuclease-Free Water

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Detail

  • ASTM Type I Water
  • Produced by reverse osmosis, ion-exchange, electro-deionization, ultrafiltration, UV treatment (UVC & 185 nm), 0.1 μm membrane filtration
  • Nuclease-free, protease-free, endotoxin-free
  • Suitable for molecular biology applications (qPCR, NGS etc.)
  • Suitable for cell culture applications
  • Produced in an ISO certified GMP laboratory
  • Water system is routinely monitored for compliance with QC standards

The Norgen water purification system has been designed in conjunction with experts from Millipore Sigma. The Ultrapure NFW is purified in a two-stage process. The first stage, to produce type II water, includes pre-treatment with activated charcoal and polyphosphate, this water then undergoes reverse osmosis, electro-deionization and ion exchange to remove contaminants as well as UV treatment for disinfection. The Pure water then flows to a second system in a clean room with an additional 4 layers of purification. After passing through an additional ion exchange column, the water is then subjected to Ultrafiltration to remove particulates, bacteria, & ionic contaminants down to trace level, the water is then treated with UV light (182 nm wavelength) to break down organic matter. Lastly a second Ultrafilter for the production of pyrogen-, nuclease- and bacteria-free water at 18.2 MΩ. This water is of utmost purity and suitable for direct use in molecular biology applications such as qPCR and NGS, as well as cell culture and other applications.

Product Specifications
pHn/a (highly pure water does not contain enough ions for an exact pH determination.)
Recommended StorageRoom Temperature
GradeUltrapure (ASTM Type I)
Biological ActivityDNase-, RNase-, Protease-, Endotoxin- Free
TOCs< 50 ppb
Resistivity> 18 MΩ-cm
TreatmentNot DEPC-Treated
Quantity100 mL, 500 mL, 1000 mL
Purification MethodsActivated charcoal, polyphosphate, reverse osmosis, electro-deionization, ion exchange, UV, ultrafiltration
Shipping ConditionsRoom Temperature

Details

Applications
For use in any molecular biology application

Storage Conditions
Norgen’s Nuclease-Free Water should be stored at room temperature. Storage at +4°C or -20°C is optional.

Precautions and Disclaimers
This product is designed for research purposes only.  It is not intended for human or diagnostic use.

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