This kit is sufficient for 150 reactions: For characterizing cyanobacteria in environmental samples Use in combination with Attogene Algae DNA isolation kit Universal 23s PCR primers Perfect for Environmental DNA (eDNA) Characterization
Detail
This kit is sufficient for 150 reactions:
For characterizing cyanobacteria in environmental samples
Use in combination with Attogene Algae DNA isolation kit
Universal 23s PCR primers
Perfect for Environmental DNA (eDNA) Characterization
The 23S ribosomal RNA (rRNA) is a crucial component of the bacterial/archean ribosome. The 23S rRNA is a 2,904-nucleotide long component of the large subunit (50S) of the ribosome. Compared to 16S rRNA genes, 23S rRNA genes have greater sequence variations due to unique insertions, deletions, and length. Attogene’s 23s PCR kit is designed for identification of specific strains of bacterial/archean using the ribosomal RNA gene region for use in Phylogenetic studies of bacterial diversity from environmental DNA samples such as water. For example, a sample of algae is obtained and washed to extract a clean algal genomic DNA (gDNA) sample. A reaction mixture is assembled from primers, master mix, and gDNA samples as required. The qPCR machine of choice is set up and loaded as needed and the mixture undergoes PCR amplification. The Primer mix provided exploits the Taq polymerase to amplify the gene region of interest.
Other Products
D6311-F-96 MagPure Blood DNA Precast Kit (96 channel machine)
Product Info
Document
Product Info
Introduction
This product provides fast and easy methods for purification of total DNA for reliable PCR and southern blotting. Total DNA(e.g., genomic, viral, mitochondrial) can be purified from whole blood, plasma, serum, buffy coat, bone marrow, other body fluids, lymphocytes, cultured cells.
Relevant reagents were pre-packed in 96-well plates in accordance with the optimal protocol.
Details
Specifications
Features
Specifications
Main Functions
Isolation total DNA from 200μl whole blood
Applications
PCR, southern bolt and virus detection, etc
Purification technology
Magnetic beads technology
Process method
Manual or automatic
Sample type
Anticoagulant blood, concentrated blood, buffy coat, lymphocytes and cultured cells
Sample amount
200μl
Elution volume
≥50μl
Time per run
≤60 minutes
Principle
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by Elution Buffer.
Advantages
High binding force – suitable for handling DNA rich samples, such as whole blood, buffy coat, concentrated blood, etc.
Fast – polydisperse magnetic beads, fast magnetic response and short extraction time
High purity – the obtained DNA can be directly used for second-generation sequencing, PCR based detection, gene bank, etc.
Automatic – saving time andlabor and safer
Kit Contents
Contents
D631101
D631102
D631103
Purification Times
48
96
480
MagPure Particles
1.2 ml
2.5 ml
11 ml
Proteinase K
24 mg
50 mg
220 mg
Protease Dissolve Buffer
1.8 ml
5 ml
15 ml
Buffer AL
15 ml
30 ml
120 ml
Buffer BD
5 ml
10 ml
50 ml
Buffer GW1
22 ml
53 ml
220 ml
Elution Buffer
15 ml
30 ml
100 ml
Storage and Stability
Proteinase K, MagPure Particles should be stored at 2-8°C upon arrival. However, short-term storage(up to 24 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.The entire kit can be stored at 2-8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.
Document
This product provides fast and easy methods for purification of total DNA for reliable PCR and southern blotting. Total DNA(e.g., genomic, viral, mitochondrial) can be purified from whole blood, plasma, serum, buffy coat, bone marrow, other body fluids, lymphocytes, cultured cells.
Relevant reagents were pre-packed in 96-well plates in accordance with the optimal protocol.
Permagen’s 50 mL Centrifuge Tube Magnetic rack is designed for magnetic bead separations from up to six, 50 mL Centrifuge tubes
Accommodates any common 50 mL Centrifuge Tubes
Beads will be pulled to back wall allowing easy aspiration and tip tracking down the front wall of the tubes without disturbing bead pellet
Features include solid aluminum alloy design with hard coat finish for years of trouble-free use, rubber feet to help prevent slipping on work bench, less tippy than common plastic products, and fast separations using any magnetic beads
endo-BCN-PEG8-PFP ester is a PEG linker featuring a BCN group and a PFP ester. BCN is a click chemistry handle used to react with azides on target molecules while the PFP is a good leaving group which is readily displaced by amines to form amides.
Document
endo-BCN-PEG8-PFP ester is a PEG linker featuring a BCN group and a PFP ester. BCN is a click chemistry handle used to react with azides on target molecules while the PFP is a good leaving group which is readily displaced by amines to form amides.
